ADAM28 (NM_021777) Human Untagged Clone

CAT#: SC112924

ADAM28 (untagged)-Human ADAM metallopeptidase domain 28 (ADAM28), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADAM28
• Synonyms: ADAM 28; eMDC II; eMDCII; MDC-L; MDCL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112924 sequence for NM_021777 edited (data generated by NextGen Sequencing)
  ATGTTGCAAGGTCTCCTGCCAGTCAGTCTCCTCCTCTCTGTTGCAGTAAGTGCTATAAAA
  GAACTCCCTGGGGTGAAGAAGTATGAAGTGGTTTATCCTATAAGACTTCATCCACTGCAT
  AAAAGAGAGGCCAAAGAGCCAGAGCAACAGGAACAATTTGAAACTGAATTAAAGTATAAA
  ATGACAATTAATGGAAAAATTGCAGTGCTTTATTTGAAAAAAAACAAGAACCTCCTTGCA
  CCAGGCTACACGGAAACATATTATAATTCCACTGGAAAGGAGATCACCACAAGCCCACAA
  ATTATGGATGATTGTTATTATCAAGGACATATTCTTAATGAAAAGGTTTCTGACGCTAGC
  ATCAGCACATGTAGGGGTCTAAGGGGCTACTTCAGTCAGGGGGATCAAAGATACTTTATT
  GAACCTTTAAGCCCCATACATCGGGATGGACAGGAGCATGCACTCTTCAAGTATAACCCT
  GATGAAAAGAATTATGACAGCACCTGTGGGATGGATGGTGTGTTGTGGGCCCACGATTTG
  CAGCAGAACATTGCCCTACCTGCCACCAAACTAGTAAAATTGAAAGACAGGAAGGTTCAG
  GAACATGAGAAATACATAGAATATTATTTGGTCCTGGATAATGGTGAGTTTAAAAGGTAC
  AATGAGAATCAAGATGAGATCAGAAAGAGGGTATTTGAGATGGCTAATTATGTCAACATG
  CTTTATAAAAAGCTCAATACTCATGTGGCCTTAGTTGGTATGGAAATCTGGACTGACAAG
  GATAAGATAAAGATAACCCCAAATGCAAGCTTCACCTTGGAGAATTTTTCTAAATGGAGG
  GGGAGTGTTCTCTCAAGAAGAAAGCGTCATGATATTGCTCAGTTAATCACAGCAACAGAA
  CTTGCTGGAACGACTGTGGGTCTTGCATTTATGTCTACAATGTGTTCTCCTTATTCTGTT
  GGCGTTGTTCAGGACCACAGCGATAATCTTCTTAGAGTTGCAGGGACAATGGCACATGAA
  ATGGGCCACAACTTTGGAATGTTTCATGACGACTATTCTTGCAAGTGTCCTTCTACAATA
  TGTGTGATGGACAAAGCACTGAGCTTCTATATACCCACAGACTTCAGTTCCTGCAGCCGT
  CTCAGCTATGACAAGTTTTTTGAAGATAAATTATCAAATTGCCTCTTTAATGCTCCATTG
  CCTACAGATATCATATCCACTCCAATTTGTGGGAACCAGTTGGTGGAAATGGGAGAGGAC
  TGTGATTGTGGGACATCTGAGGAATGTACCAATATTTGCTGTGATGCTAAGACATGTAAA
  ATCAAAGCAACTTTTCAATGTGCATTAGGAGAATGTTGTGAAAAATGCCAATTTAAAAAG
  GCTGGGATGGTGTGCAGACCAGCAAAAGATGAGTGCGACCTGCCTGAAATGTGTAATGGT
  AAATCTGGTAATTGTCCTGATGATAGATTCCAAGTCAATGGCTTCCCTTGCCATCACGGG
  AAGGGCCACTGCTTGATGGGGACATGCCCCACACTGCAGGAGCAGTGCACAGAGCTGTGG
  GGACCAGGTAGGAGGACAAATCCTTTCCCCTGTGCATGTGCGAAGGAAAATCATTTCAGA
  TGA
  
  Clone variation with respect to NM_021777.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021777 unedited
  TGTTANATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCCACCTGAG
  CGAGAAGAGCAGACACCGTGCTCCTGGAATCACCCAGCATGTTGCAAGGTCTCCTGCCAG
  TCAGTCTCCTCCTCTCTGTTGCAGTAAGTGCTATAAAAGAACTCCCTGGGGTGAAGAAGT
  ATGAAGTGGTTTATCCTATAAGACTTCATCCACTGCATAAAAGAGAGGCCAAAGAGCCAG
  AGCAACAGGAACAATTTGAAACTGAATTAAAGTATAAAATGACAATTAATGGAAAAATTG
  CAGTGCTTTATTTGAAAAAAAACAAGAACCTCCTTGCACCAGGCTACACGGAAACATATT
  ATAATTCCACTGGAAAGGAGATCACCACAAGCCCACAAATTATGGATGATTGTTATTATC
  AAGGACATATTCTTAATGAAAAGGTTTCTGACGCTAGCATCAGCACATGTAGGGGTCTAA
  GGGGCTACTTCAGTCAGGGGGATCAAAGATACTTTATTGAACCTTTAAGCCCCATACATC
  GGGATGGACAGGAGCATGCACTCTTCAAGTATAACCCTGATGAAAAGAATTATGACAGCA
  CCTGTGGGATGGATGGTGTGTTGTGGGCCCACGATTTGCAGCAGAACATTGCCCTACCTG
  CCACCAAACTAGTAAAATTGAAAGACAGGAAGGTTCAGGAACATGAGAAATACATAGAAT
  ATTATTTGGTCCTGGATAATGGTGAGTTTAAAAGGTACAATGAGAATCAAGATGAGATCA
  GANAGAGGGTATTTGAGATGGCTAATTATGTCAACATGCTNNTATAAAAGCTCAATACTC
  ATGTGGCCCTAGTTGGTATGGAAATCTGGNACTGACAGGGATAGATAAGATAACCCAAAT
  GCAGCTTCACCTTGGAGAATTTTCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021777 unedited
  TTGCTATGCCACGCGGCACGCATTCTAAGATCGGTTTTTTTTTTTTTTTTTTCTTTTTTT
  TTTTTTTTTTTTGGAGACGGAGTCTGGCTTTGTCACCCAGGCTGGAGTGCAGTGGCACGA
  TCTCTGCTGACGGCAAGCTCCGCCTCCTGGGTTCAGGCCCTTCTCCTGCCTCAGCCTCCC
  GAGTAGCTGGGACAGGCGCCCACTAGCACGCCCGGCTAATTTTTTGGATTTTTAGTAGAG
  ACGGGGTTTCACCGTGTTAGCCAGGATGGGCTTGATCTCCTGACCTCGTGATCCGCCCGC
  CTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCGCACCTGGCCAAAGTTATT
  TAATACTATACAAATTGCTGTTTAAATTTTCGGATATCTCTTTAAACTTTTATTTTTCAA
  GTTTTCTTGGTGAAATTAGAGAATATGGTCTCTAAAATTTTGGCATTATGGACAAAGCGA
  CCATTTAAGAACTTTCACTTTTCTTTATTGGCTTTCTGCATTTCCAGAGTTCTTTTCTTT
  TTTCCATCTTTTCTTATTTCTGGCCCATCCCACAGTACTTCTGCCACCCCTGCACACACA
  CACACACACACACACCCGGACACACACAGATTTCCAAGGGTTTATGATCTTAATAGGGTT
  GCCAGATGAAAAGAAAAAGACACCCCAGTTTAATTTGAATTTCAGAAACACAAATAATTT
  TAGTATGAGCACACCTCATATATTGCATGGGATATACTGATGCTGAGACAAAAACATAAA
  TTACAAAAACTTGTAATTTTAGTTAAAATTAACGGGCCATCCTTATTTTACTGCTAAACT
  AGCACCCTAAACCACCTCAACCTAAACCTTCCATGATTGAATCGAGCTCTAGAAGATAGA
  CGATGGCCTTGACTGGTAACATGCATCTGAAGATTTCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021777
• ORF Size: 1623 bp
• Insert Size: 3000
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_021777.2, NP_068547.2
• RefSeq Size: 2076
• RefSeq ORF: 1623
• Locus ID: 10863
• Domains: Reprolysin, DISIN, Pep_M12B_propep, ACR
• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. This gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (3, also known as MDC-Ls) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus, compared to isoform 1.