Integrin alpha 3 (ITGA3) (NM_002204) Human Tagged ORF Clone

CAT#: RC217882

ITGA3 (Myc-DDK-tagged)-Human integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) (ITGA3), transcript variant a

Product Images

Western blot validation of overexpression lysate (Cat# LY419471) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217882 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ITGA3
• Synonyms: CD49C; FRP-2; GAP-B3; GAPB3; ILNEB; MSK18; VCA-2; VL3A; VLA3a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217882 representing NM_002204
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCCGGCCCCAGCCGCGCGCCCCGCGCCCCACGCCTGATGCTCTGTGCGCTCGCCTTGATGGTGG
  CGGCCGGCGGCTGCGTCGTCTCCGCCTTCAACCTGGATACCCGATTCCTGGTAGTGAAGGAGGCCGGGAA
  CCCGGGCAGCCTCTTCGGCTACTCGGTCGCCCTCCATCGGCAGACAGAGCGGCAGCAGCGCTACCTGCTC
  CTGGCTGGTGCCCCCCGGGAGCTCGCTGTGCCCGATGGCTACACCAACCGGACTGGTGCTGTGTACCTGT
  GCCCACTCACTGCCCACAAGGATGACTGTGAGCGGATGAACATCACAGTGAAAAATGACCCTGGCCATCA
  CATTATTGAGGACATGTGGCTTGGAGTGACTGTGGCCAGCCAGGGCCCTGCAGGCAGAGTTCTGGTCTGT
  GCCCACCGCTACACCCAGGTGCTGTGGTCAGGGTCAGAAGACCAGCGGCGCATGGTGGGCAAGTGCTACG
  TGCGAGGCAATGACCTAGAGCTGGACTCCAGTGATGACTGGCAGACCTACCACAACGAGATGTGCAATAG
  CAACACAGACTACCTGGAGACGGGCATGTGCCAGCTGGGCACCAGCGGTGGCTTCACCCAGAACACTGTG
  TACTTCGGCGCCCCCGGTGCCTACAACTGGAAAGGAAACAGCTACATGATTCAGCGCAAGGAGTGGGACT
  TATCTGAGTATAGTTACAAGGACCCAGAGGACCAAGGAAACCTCTATATTGGGTACACGATGCAGGTAGG
  CAGCTTCATCCTGCACCCCAAAAACATCACCATTGTGACAGGTGCCCCACGGCACCGACATATGGGCGCG
  GTGTTCTTGCTGAGCCAGGAGGCAGGCGGAGACCTGCGGAGGAGGCAGGTGCTGGAGGGCTCGCAGGTGG
  GCGCCTATTTTGGCAGCGCCATTGCCCTGGCAGACCTGAACAATGATGGGTGGCAGGACCTCCTGGTGGG
  CGCCCCCTACTACTTTGAGAGGAAAGAGGAAGTAGGGGGTGCCATCTATGTCTTCATGAACCAGGCGGGA
  ACCTCCTTCCCTGCTCACCCCTCACTCCTTCTTCATGGCCCCAGTGGCTCTGCCTTTGGTTTATCTGTGG
  CCAGCATTGGTGACATCAACCAGGATGGATTTCAGGATATTGCTGTGGGAGCTCCGTTTGAAGGCTTGGG
  CAAAGTGTACATCTATCACAGTAGCTCTAAGGGGCTCCTCAGACAGCCCCAGCAGGTAATCCATGGAGAG
  AAGCTGGGACTGCCTGGGTTGGCCACCTTCGGCTATTCCCTCAGTGGGCAGATGGATGTGGATGAGAACT
  TCTACCCAGACCTTCTAGTGGGAAGCCTGTCAGACCACATTGTGCTGCTGCGGGCCCGGCCCGTCATCAA
  CATCGTCCACAAGACCTTGGTGCCCAGGCCAGCTGTGCTGGACCCTGCACTTTGCACGGCCACCTCTTGT
  GTGCAAGTGGAGCTGTGCTTTGCTTACAACCAGAGTGCCGGGAACCCCAACTACAGGCGAAACATCACCC
  TGGCCTACACTCTGGAGGCTGACAGGGACCGCCGGCCGCCCCGGCTCCGCTTTGCCGGCAGTGAGTCCGC
  TGTCTTCCACGGCTTCTTCTCCATGCCCGAGATGCGCTGCCAGAAGCTGGAGCTGCTCCTGATGGACAAC
  CTCCGTGACAAACTCCGCCCCATCATCATCTCCATGAACTACTCTTTACCTTTGCGGATGCCCGATCGCC
  CCCGGCTGGGGCTGCGGTCCCTGGACGCCTACCCGATCCTCAACCAGGCACAGGCTCTGGAGAACCACAC
  TGAGGTCCAGTTCCAGAAGGAGTGCGGGCCTGACAACAAGTGTGAGAGCAACTTGCAGATGCGGGCAGCC
  TTCGTGTCAGAGCAGCAGCAGAAGCTGAGCAGGCTCCAGTACAGCAGAGACGTCCGGAAATTGCTCCTGA
  GCATCAACGTGACGAACACCCGGACCTCGGAGCGCTCCGGGGAGGACGCCCACGAGGCGCTGCTCACCCT
  GGTGGTGCCTCCCGCCCTGCTGCTGTCCTCAGTGCGCCCCCCCGGGGCCTGCCAAGCTAATGAGACCATC
  TTTTGCGAGCTGGGGAACCCCTTCAAACGGAACCAGAGGATGGAGCTGCTCATCACCTTTGAGGTCATCG
  GGGTGACCCTGCACACAAGGGACCTTCAGGTGCAGCTGCAGCTCTCCACGTCGAGTCACCAGGACAACCT
  GTGGCCCATGATCCTCACTCTGCTGGTGGACTATACACTCCAGACCTCGCTTAGCATGGTAAATCACCGG
  CTACAAAGCTTCTTTGGGGGGACAGTGATGGGTGAGTCTGGCATGAAAACTGTGGAGGATGTAGGAAGCC
  CCCTCAAGTATGAATTCCAGGTGGGCCCAATGGGGGAGGGGCTGGTGGGCCTGGGGACCCTGGTCCTAGG
  TCTGGAGTGGCCCTACGAAGTCAGCAATGGCAAGTGGCTGCTGTATCCCACGGAGATCACCGTCCATGGC
  AATGGGTCCTGGCCCTGCCGACCACCTGGAGACCTTATCAACCCTCTCAACCTCACTCTTTCTGACCCTG
  GGGACAGGCCATCATCCCCACAGCGCAGGCGGCGACAGCTGGATCCAGGGGGAGGCCAGGGCCCCCCACC
  TGTCACTCTGGCTGCTGCCAAAAAAGCCAAGTCTGAGACTGTGCTGACCTGTGCCACAGGGCGTGCCCAC
  TGTGTGTGGCTAGAGTGCCCCATCCCTGATGCCCCCGTTGTCACCAACGTGACTGTGAAGGCACGAGTGT
  GGAACAGCACCTTCATCGAGGATTACAGAGACTTTGACCGAGTCCGGGTAAATGGCTGGGCTACCCTATT
  CCTCCGAACCAGCATCCCCACCATCAACATGGAGAACAAGACCACGTGGTTCTCTGTGGACATTGACTCG
  GAGCTGGTGGAGGAGCTGCCGGCCGAAATCGAGCTGTGGCTGGTGCTGGTGGCCGTGGGTGCAGGGCTGC
  TGCTGCTGGGGCTGATCATCCTCCTGCTGTGGAAGTGCGGCTTCTTCAAGCGAGCCCGCACTCGCGCCCT
  GTATGAAGCTAAGAGGCAGAAGGCGGAGATGAAGAGCCAGCCGTCAGAGACAGAGAGGCTGACCGACGAC
  TAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217882 representing NM_002204
  Red=Cloning site Green=Tags(s)
  
  MGPGPSRAPRAPRLMLCALALMVAAGGCVVSAFNLDTRFLVVKEAGNPGSLFGYSVALHRQTERQQRYLL
  LAGAPRELAVPDGYTNRTGAVYLCPLTAHKDDCERMNITVKNDPGHHIIEDMWLGVTVASQGPAGRVLVC
  AHRYTQVLWSGSEDQRRMVGKCYVRGNDLELDSSDDWQTYHNEMCNSNTDYLETGMCQLGTSGGFTQNTV
  YFGAPGAYNWKGNSYMIQRKEWDLSEYSYKDPEDQGNLYIGYTMQVGSFILHPKNITIVTGAPRHRHMGA
  VFLLSQEAGGDLRRRQVLEGSQVGAYFGSAIALADLNNDGWQDLLVGAPYYFERKEEVGGAIYVFMNQAG
  TSFPAHPSLLLHGPSGSAFGLSVASIGDINQDGFQDIAVGAPFEGLGKVYIYHSSSKGLLRQPQQVIHGE
  KLGLPGLATFGYSLSGQMDVDENFYPDLLVGSLSDHIVLLRARPVINIVHKTLVPRPAVLDPALCTATSC
  VQVELCFAYNQSAGNPNYRRNITLAYTLEADRDRRPPRLRFAGSESAVFHGFFSMPEMRCQKLELLLMDN
  LRDKLRPIIISMNYSLPLRMPDRPRLGLRSLDAYPILNQAQALENHTEVQFQKECGPDNKCESNLQMRAA
  FVSEQQQKLSRLQYSRDVRKLLLSINVTNTRTSERSGEDAHEALLTLVVPPALLLSSVRPPGACQANETI
  FCELGNPFKRNQRMELLITFEVIGVTLHTRDLQVQLQLSTSSHQDNLWPMILTLLVDYTLQTSLSMVNHR
  LQSFFGGTVMGESGMKTVEDVGSPLKYEFQVGPMGEGLVGLGTLVLGLEWPYEVSNGKWLLYPTEITVHG
  NGSWPCRPPGDLINPLNLTLSDPGDRPSSPQRRRRQLDPGGGQGPPPVTLAAAKKAKSETVLTCATGRAH
  CVWLECPIPDAPVVTNVTVKARVWNSTFIEDYRDFDRVRVNGWATLFLRTSIPTINMENKTTWFSVDIDS
  ELVEELPAEIELWLVLVAVGAGLLLLGLIILLLWKCGFFKRARTRALYEAKRQKAEMKSQPSETERLTDD
  Y
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002204
• ORF Size: 3153 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002204.1, NM_002204.2, NM_002204.3, NP_002195.1
• RefSeq Size: 4637 bp
• RefSeq ORF: 3156 bp
• Locus ID: 3675
• Cytogenetics: 17q21.33
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Pathways in cancer, Regulation of actin cytoskeleton, Small cell lung cancer
• MW: 116.64 kDa
• Gene Summary: 'The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function as cell surface adhesion molecules. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 3 subunit. This subunit joins with a beta 1 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. Expression of this gene may be correlated with breast cancer metastasis. [provided by RefSeq, Oct 2015]'