POLR3G (NM_006467) Human Tagged ORF Clone

CAT#: RC217994

POLR3G (Myc-DDK-tagged)-Human polymerase (RNA) III (DNA directed) polypeptide G (32kD) (POLR3G)

Product Images

Western blot validation of overexpression lysate (Cat# LY416626) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217994 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POLR3G
• Synonyms: RPC7; RPC32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217994 representing NM_006467
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGAATAAAGGAAGAGGACGTGCTGCTTATACCTTTAATATTGAGGCTGTTGGATTTAGCAAAG
  GTGAAAAGTTACCTGATGTAGTGTTGAAACCACCCCCACTATTTCCTGATACAGATTATAAACCAGTGCC
  ACTGAAAACAGGAGAAGGTGAAGAATATATGCTGGCTTTGAAACAGGAGTTGAGAGAAACAATGAAAAGA
  ATGCCTTATTTTATTGAAACACCTGAAGAAAGACAAGATATTGAAAGGTATAGTAAAAGATACATGAAGG
  TATACAAGGAAGAATGGATACCAGATTGGAGAAGACTTCCAAGAGAGATGATGCCAAGAAATAAATGTAA
  AAAAGCAGGCCCAAAACCCAAAAAGGCAAAAGACGCAGGCAAAGGCACACCACTCACTAATACTGAAGAT
  GTGTTGAAAAAAATGGAGGAATTGGAAAAAAGAGGTGATGGTGAAAAATCAGATGAGGAAAATGAAGAGA
  AAGAAGGAAGCAAAGAGAAAAGTAAAGAAGGTGATGATGACGATGACGATGATGCCGCAGAACAGGAGGA
  ATATGATGAAGAAGAGCAAGAAGAGGAAAATGACTACATTAATTCATACTTTGAAGATGGAGATGATTTT
  GGCGCAGACAGTGATGACAACATGGATGAGGCAACCTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217994 representing NM_006467
  Red=Cloning site Green=Tags(s)
  
  MAGNKGRGRAAYTFNIEAVGFSKGEKLPDVVLKPPPLFPDTDYKPVPLKTGEGEEYMLALKQELRETMKR
  MPYFIETPEERQDIERYSKRYMKVYKEEWIPDWRRLPREMMPRNKCKKAGPKPKKAKDAGKGTPLTNTED
  VLKKMEELEKRGDGEKSDEENEEKEGSKEKSKEGDDDDDDDAAEQEEYDEEEQEEENDYINSYFEDGDDF
  GADSDDNMDEATY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006467
• ORF Size: 669 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006467.1, NM_006467.2, NP_006458.1, NP_006458.2
• RefSeq Size: 1000
• RefSeq ORF: 672
• Locus ID: 10622
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• MW: 25.7 kDa