XRCC4 (NM_022550) Human Tagged ORF Clone

CAT#: RC218029L1

Lenti ORF clone of Human X-ray repair complementing defective repair in Chinese hamster cells 4 (XRCC4), transcript variant 3, Myc-DDK-tagged

Product Images

Double digestion of RC218029L1 using SgfI and MluI

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: XRCC4
• Synonyms: SSMED
• Vector: pLenti-C-Myc-DDK
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC218029).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_022550
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022550.1, NP_072044.1
• RefSeq Size: 1707 bp
• RefSeq ORF: 1005 bp
• Locus ID: 7518
• UniProt ID: Q13426, A0A024RAL0, Q7Z763
• Cytogenetics: 5q14.2
• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining
• MW: 37.9 kDa
• Gene Summary: 'The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site. [provided by RefSeq, Oct 2019]'