XRCC4 (NM_022550) Human Untagged Clone

CAT#: SC126125

XRCC4 (untagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 4 (XRCC4), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: XRCC4
• Synonyms: SSMED
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022550 edited
  GGGGACCGGAAGTAGAGTCACGGAGAGGTAGGATCCGGAAGTGGGGCTGCCTCTTTAAAT
  AACAAAAATCTGAGGTATTAAGAAATGGAGAGAAAAATAAGCAGAATCCACCTTGTTTCT
  GAACCCAGTATAACTCATTTTCTACAAGTATCTTGGGAGAAAACACTGGAATCTGGTTTT
  GTTATTACACTTACTGATGGTCATTCAGCATGGACTGGGACAGTTTCTGAATCAGAGATT
  TCCCAAGAAGCTGATGACATGGCAATGGAAAAAGGGAAATATGTTGGTGAACTGAGAAAA
  GCATTGTTGTCAGGAGCAGGACCAGCTGATGTATACACGTTTAATTTTTCTAAAGAGTCT
  TGTTATTTCTTCTTTGAGAAAAACCTGAAAGATGTCTCATTCAGACTTGGTTCCTTCAAC
  CTAGAGAAAGTTGAAAACCCAGCTGAAGTCATTAGAGAACTTATTTGTTATTGCTTGGAC
  ACCATTGCAGAAAATCAAGCCAAAAATGAGCACCTGCAGAAAGAAAATGAAAGGCTTCTG
  AGAGATTGGAATGATGTTCAAGGACGATTTGAAAAATGTGTGAGTGCTAAGGAAGCTTTG
  GAGACTGATCTTTATAAGCGGTTTATTCTGGTGTTGAATGAGAAGAAAACAAAAATCAGA
  AGTTTGCATAATAAATTATTAAATGCAGCTCAAGAACGAGAAAAGGACATCAAACAAGAA
  GGGGAAACTGCAATCTGTTCTGAAATGACTGCTGACCGAGATCCAGTCTATGATGAGAGT
  ACTGATGAGGAAAGTGAAAACCAAACTGATCTCTCTGGGTTGGCTTCAGCTGCTGTAAGT
  AAAGATGATTCCATTATTTCAAGTCTTGATGTCACTGATATTGCACCAAGTAGAAAAAGG
  AGACAGCGAATGCAAAGAAATCTTGGGACAGAACCTAAAATGGCTCCTCAGGAGAATCAG
  CTTCAAGAAAAGGAAAATTCTAGGCCTGATTCTTCACTACCTGAGACGTCTAAAAAGGAG
  CACATCTCAGCTGAAAACATGTCTTTAGAAACTCTGAGAAACAGCAGCCCAGAAGACCTC
  TTTGATGAGATTTAACAGTCTCAAAAAATACTTTGATGTTCACTAGACTATGTTTTCTAT
  TCATTTCTTTAAAATGAAAAAGGAGAATTTCAAGTCAGCAGCCGCTATTACCGTATCTTA
  CAATTTAATTACATACACAGTGAATTGAAACCATTGTGCAAAATGGATTACACATGTATA
  CAAAGATACGATTTGATGATGACACTGGCACATTATTCTAAACTATTCATTCAGCATGCC
  TATAATTACATAAATTGTATGAGACTTTTTGTTGCAAAGGACACATTTATCATATTCATT
  CACACATATTATATGTGATAGCTGTCCAACATCCTGTCTGGGAAGATTTTGAAAACAGGA
  CAAAGAAAACATCACTTTAAAATGTCTTCAGCTTTTTTTGAATAGACGTATTCAAACATA
  TTCTGAACATTGATGTTTGAACATTTTAATTTGTGTGATGATGTAGAAAATATAATTTTA
  GTTTGTACATAAACATTGTGAAAATCTGATAATAAAATTTTTGATACACTAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022550 unedited
  CCCGCGGTTCAGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGCAATTCGCCATTA
  CGGCCGGGGACCGCGAAGTAGATCACGGAGGGGTGGGATCCGGGAGTGGGGCTGCCTCTT
  TAAATAACAAAAATCTGAGGTATTAAGAAATGGAGAGAAAAATAAGCAGAATCCACCTTG
  TTTCTGAACCCAGTATAACTCATTTTCTACAAGTATCTTGGGAGAAAACACTGGAATCTG
  GTTTTGTTATGACACTTACTGATGGTCATTCAGCATGGACTGGGACAGTTTCTGAATCAG
  AGATTTCCCAAGAAGCTGATGACATGGCAATGGAAAAAGGGAAATATGTTGGTGAACTGA
  GAAAAGCATTGTTGTCAGGAGCAGGACCAGCTGATGTATACACGTTTAATTTTTCTAAAG
  AGTCTTGTTATTTCTTCTTTGAGAAAAACCTGAAAGATGTCTCATTCAGACTTGGTTCCT
  TCAACCTAGAGAAAGTTGAAAACCCAGCTGAAGTCATTAGAGAACTTATTTGTTATTGCT
  TGGACACCATTGCAGAAAATCAAGCCAAAAATGAGCACCTGCAGAAAGAAAATGAAAGGC
  TTCTGAGAGATTGGAATGATGTTCAAGGACGATTTGAAAAATGTGTGAGTGCTAAGGAAG
  CTTTGGAGACTGATCTTTATAAGCGGTTTATTCTGGTGTTGAATGAGAAGAANACAAAAT
  CAGAAGTTTGCATAATAAATTATTAAATGCAGCTCAAGGACGAGGAAAAGACATCAAACA
  AGAAGGGGAAACTGCAATCTGTTCTGAAATGACTGCTGACCGAGTCCAGTCTATGATGAG
  AGTACTGATGAGGGAAGTGAAAACCAACTGATCTCTCTGGGGTGGCTTCAGCTGCTGTAA
  GTAAGATAN
  
• Restriction Sites: Please inquire
• ACCN: NM_022550
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022550.1, NP_072044.1
• RefSeq Size: 1707 bp
• RefSeq ORF: 1005 bp
• Locus ID: 7518
• Cytogenetics: 5q14.2
• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining
• Gene Summary: 'The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site. [provided by RefSeq, Oct 2019]'
  Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1 and 3 encode the same isoform (1).