EYA1 (NM_172059) Human Tagged ORF Clone

CAT#: RC218031

EYA1 (Myc-DDK-tagged)-Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY406817) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218031 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EYA1
• Synonyms: BOP; BOR; BOS1; OFC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218031 representing NM_172059
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATGCAGGATCTAACCAGCCCGCATAGCCGTCTGAGTGGTAGTAGTGAATCCCCCAGTGGCCCCA
  AACTCGGTAACTCTCATATAAATAGTAATTCCATGACTCCCAATGGCACCGAAGTTAAAACAGAGCCAAT
  GAGCAGCAGTGAAACAGCTTCAACGACAGCCGACGGGTCTTTAAACAATTTCTCAGGTTCAGCAATTGGG
  AGCAGTAGTTTCAGCCCACGACCAACTCACCAGTTCTCTCCACCACAGATTTACCCTTCCAACAGACCAT
  ACCCACATATTCTCCCTACCCCTTCCTCACAAACTATGGCTGCATATGGGCAAACACAGTTTACCACAGG
  AATGCAACAAGCTACAGCCTATGCCACGTACCCACAGCCAGGACAGCCGTACGGCATTTCCTCATATGGC
  ATCAAGACTGAAGGTGGATTGTCACAGTCTCAGTCACCTGGACAGACAGGATTTCTCAGCTATGGCACAA
  GCTTCAGTACCCCTCAACCTGGACAGGCACCATACAGCTACCAGATGCAAGGTAGCAGTTTTACAACATC
  ATCAGGAATATATACAGGAAATAATTCACTCACAAATTCCTCTGGATTTAATAGTTCACAGCAGGACTAT
  CCGTCTTATCCCAGTTTTGGCCAGGGTCAGTACGCACAGTATTATAACAGCTCACCGTATCCAGCACATT
  ATATGACCAGCAGCAACACCAGCCCAACGACACCATCCACCAATGCCACTTACCAGCTTCAAGAACCGCC
  ATCTGGCATCACCAGCCAAGCAGTTACAGATCCCACAGCAGAGTACAGCACAATCCACAGCCCATCAACA
  CCCATTAAAGATTCAGATTCTGATCGATTGCGTCGAGGTTCAGATGGGAAATCACGTGGACGGGGCCGAA
  GAAACAATAATCCTTCACCTCCCCCAGATTCTGATCTTGAGAGAGTGTTCATCTGGGACTTGGATGAGAC
  AATCATTGTTTTCCACTCCTTGCTTACTGGGTCCTACGCCAACAGATATGGGAGGGAATGTGACCAAGTC
  CATATAGATGATGTTTCTTCAGATGATAACGGACAGGACCTAAGCACATATAACTTTGGAACAGATGGCT
  TTCCTGCTGCAGCAACCAGTGCTAACTTATGTTTGGCAACTGGTGTACGGGGCGGTGTGGACTGGATGAG
  AAAGTTGGCCTTCCGCTACAGACGGGTAAAAGAGATCTACAACACCTACAAAAATAATGTTGGAGGTCTG
  CTTGGTCCAGCTAAGAGGGAAGCCTGGCTGCAGTTGAGGGCCGAAATTGAAGCCCTGACCGACTCCTGGT
  TGACACTGGCCCTGAAAGCACTCTCGCTCATTCACTCCCGGACAAACTGTGTGAATATTTTAGTAACAAC
  TACTCAGCTCATCCCAGCATTGGCGAAAGTCCTGCTGTATGGGTTAGGAATTGTATTTCCAATAGAAAAT
  ATTTACAGTGCAACTAAAATAGGAAAAGAAAGCTGTTTTGAGAGAATAATTCAAAGGTTTGGAAGAAAAG
  TGGTGTATGTTGTTATAGGAGATGGTGTAGAAGAAGAACAAGGAGCAAAAAAGCACGCGATGCCCTTCTG
  GAGGATCTCCAGCCACTCGGACCTCATGGCCCTGCACCATGCCTTGGAACTGGAGTACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218031 representing NM_172059
  Red=Cloning site Green=Tags(s)
  
  MEMQDLTSPHSRLSGSSESPSGPKLGNSHINSNSMTPNGTEVKTEPMSSSETASTTADGSLNNFSGSAIG
  SSSFSPRPTHQFSPPQIYPSNRPYPHILPTPSSQTMAAYGQTQFTTGMQQATAYATYPQPGQPYGISSYG
  IKTEGGLSQSQSPGQTGFLSYGTSFSTPQPGQAPYSYQMQGSSFTTSSGIYTGNNSLTNSSGFNSSQQDY
  PSYPSFGQGQYAQYYNSSPYPAHYMTSSNTSPTTPSTNATYQLQEPPSGITSQAVTDPTAEYSTIHSPST
  PIKDSDSDRLRRGSDGKSRGRGRRNNNPSPPPDSDLERVFIWDLDETIIVFHSLLTGSYANRYGRECDQV
  HIDDVSSDDNGQDLSTYNFGTDGFPAAATSANLCLATGVRGGVDWMRKLAFRYRRVKEIYNTYKNNVGGL
  LGPAKREAWLQLRAEIEALTDSWLTLALKALSLIHSRTNCVNILVTTTQLIPALAKVLLYGLGIVFPIEN
  IYSATKIGKESCFERIIQRFGRKVVYVVIGDGVEEEQGAKKHAMPFWRISSHSDLMALHHALELEYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172059
• ORF Size: 1671 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172059.1, NM_172059.2, NM_172059.3, NP_742056.1
• RefSeq Size: 3610 bp
• RefSeq ORF: 1761 bp
• Locus ID: 2138
• Cytogenetics: 8q13.3
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• MW: 60.5 kDa
• Gene Summary: 'This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Dec 2013]'