LBX1 (NM_006562) Human Tagged ORF Clone

CAT#: RC218048

LBX1 (Myc-DDK-tagged)-Human ladybird homeobox 1 (LBX1)

Product Images

Western blot validation of overexpression lysate (Cat# LY416561) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218048 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LBX1 protein (Cat# TP318048). The protein was produced from HEK293T cells transfected with LBX1 cDNA clone (Cat# RC218048) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LBX1
• Synonyms: homeobox; HPX-6; HPX6; LBX1H
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218048 representing NM_006562
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCCAAGGAGGACGGCAAGGCGGCGCCGGGGGAGGAGCGGCGGCGCAGCCCGCTGGACCACCTGC
  CTCCGCCTGCCAACTCCAACAAGCCACTGACGCCGTTCAGCATCGAGGACATCCTCAACAAGCCGTCTGT
  GCGGAGAAGTTACTCGCTGTGCGGGGCGGCGCACCTGCTGGCCGCCGCGGACAAGCACGCGCAGGGCGGC
  TTGCCCCTGGCGGGCCGCGCGCTGCTCTCGCAGACCTCGCCGCTGTGCGCGCTGGAGGAGCTCGCCAGCA
  AGACGTTTAAGGGGCTGGAGGTCAGCGTTCTGCAGGCAGCCGAAGGCCGCGACGGTATGACCATCTTTGG
  GCAGCGGCAGACCCCTAAGAAGCGGCGAAAGTCGCGCACGGCCTTCACCAACCACCAGATCTATGAATTG
  GAAAAGCGCTTTCTATACCAGAAGTACCTGTCCCCCGCCGATCGCGACCAAATCGCGCAGCAGCTGGGCC
  TCACCAACGCGCAAGTCATCACCTGGTTCCAGAATCGGCGCGCTAAGCTCAAGCGGGACCTGGAGGAGAT
  GAAGGCCGACGTAGAGTCCGCCAAGAAACTGGGCCCCAGCGGGCAGATGGACATCGTGGCGCTGGCCGAA
  CTCGAGCAGAACTCGGAGGCCACAGCCGGCGGTGGCGGCGGCTGCGGCAGGGCCAAGTCGAGGCCCGGCT
  CTCCGGTCCTCCCCCCAGGCGCCCCGAAGGCCCCGGGCGCTGGCGCCCTGCAGCTCTCGCCTGCCTCTCC
  GCTCACGGACCAGCCGGCCAGCAGCCAGGACTGCTCGGAGGACGAGGAAGACGAAGAGATCGACGTGGAC
  GAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218048 representing NM_006562
  Red=Cloning site Green=Tags(s)
  
  MTSKEDGKAAPGEERRRSPLDHLPPPANSNKPLTPFSIEDILNKPSVRRSYSLCGAAHLLAAADKHAQGG
  LPLAGRALLSQTSPLCALEELASKTFKGLEVSVLQAAEGRDGMTIFGQRQTPKKRRKSRTAFTNHQIYEL
  EKRFLYQKYLSPADRDQIAQQLGLTNAQVITWFQNRRAKLKRDLEEMKADVESAKKLGPSGQMDIVALAE
  LEQNSEATAGGGGGCGRAKSRPGSPVLPPGAPKAPGAGALQLSPASPLTDQPASSQDCSEDEEDEEIDVD
  D
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006562
• ORF Size: 843 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006562.1, NM_006562.2, NM_006562.3, NM_006562.4, NP_006553.2
• RefSeq Size: 1287
• RefSeq ORF: 846
• Locus ID: 10660
• Protein Families: Transcription Factors
• MW: 30 kDa
• Gene Summary: This gene and the orthologous mouse gene were found by their homology to the Drosophila lady bird early and late homeobox genes. In the mouse, this gene is a key regulator of muscle precursor cell migration and is required for the acquisition of dorsal identities of forelimb muscles. [provided by RefSeq, Jul 2008]