MRP1 (ABCC1) (NM_004996) Human Tagged ORF Clone

CAT#: RC218182

ABCC1 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family C (CFTR/MRP), member 1 (ABCC1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417598) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218182 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCC1
• Synonyms: ABC29; ABCC; GS-X; MRP; MRP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218182 representing NM_004996
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTCCGGGGCTTCTGCAGCGCCGATGGCTCCGACCCGCTCTGGGACTGGAATGTCACGTGGAATA
  CCAGCAACCCCGACTTCACCAAGTGCTTTCAGAACACGGTCCTCGTGTGGGTGCCTTGTTTTTACCTCTG
  GGCCTGTTTCCCCTTCTACTTCCTCTATCTCTCCCGACATGACCGAGGCTACATTCAGATGACACCTCTC
  AACAAAACCAAAACTGCCTTGGGATTTTTGCTGTGGATCGTCTGCTGGGCAGACCTCTTCTACTCTTTCT
  GGGAAAGAAGTCGGGGCATATTCCTGGCCCCAGTGTTTCTGGTCAGCCCAACTCTCTTGGGCATCACCAT
  GCTGCTTGCTACCTTTTTAATTCAGCTGGAGAGGAGGAAGGGAGTTCAGTCTTCAGGGATCATGCTCACT
  TTCTGGCTGGTAGCCCTAGTGTGTGCCCTAGCCATCCTGAGATCCAAAATTATGACAGCCTTAAAAGAGG
  ATGCCCAGGTGGACCTGTTTCGTGACATCACTTTCTACGTCTACTTTTCCCTCTTACTCATTCAGCTCGT
  CTTGTCCTGTTTCTCAGATCGCTCACCCCTGTTCTCGGAAACCATCCACGACCCTAATCCCTGCCCAGAG
  TCCAGCGCTTCCTTCCTGTCGAGGATCACCTTCTGGTGGATCACAGGGTTGATTGTCCGGGGCTACCGCC
  AGCCCCTGGAGGGCAGTGACCTCTGGTCCTTAAACAAGGAGGACACGTCGGAACAAGTCGTGCCTGTTTT
  GGTAAAGAACTGGAAGAAGGAATGCGCCAAGACTAGGAAGCAGCCGGTGAAGGTCGTGTACTCCTCCAAG
  GATCCTGCCCAGCCGAAAGAGAGTTCCAAGGTGGATGCGAATGAGGAGGTGGAGGCTTTGATCGTCAAGT
  CCCCACAGAAGGAGTGGAACCCCTCTCTGTTTAAGGTGTTATACAAGACCTTTGGGCCCTACTTCCTCAT
  GAGCTTCTTCTTCAAGGCCATCCACGACCTGATGATGTTTTCCGGGCCGCAGATCTTAAAGTTGCTCATC
  AAGTTCGTGAACGACACGAAGGCCCCAGACTGGCAGGGCTACTTCTACACCGTGCTGCTGTTTGTCACTG
  CCTGCCTGCAGACCCTCGTGCTGCACCAGTACTTCCACATCTGCTTCGTCAGTGGCATGAGGATCAAGAC
  CGCTGTCATTGGGGCTGTCTATCGGAAGGCCCTGGTGATCACCAATTCAGCCAGAAAATCCTCCACGGTC
  GGGGAGATTGTCAACCTCATGTCTGTGGACGCTCAGAGGTTCATGGACTTGGCCACGTACATTAACATGA
  TCTGGTCAGCCCCCCTGCAAGTCATCCTTGCTCTCTACCTCCTGTGGCTGAATCTGGGCCCTTCCGTCCT
  GGCTGGAGTGGCGGTGATGGTCCTCATGGTGCCCGTCAATGCTGTGATGGCGATGAAGACCAAGACGTAT
  CAGGTGGCCCACATGAAGAGCAAAGACAATCGGATCAAGCTGATGAACGAAATTCTCAATGGGATCAAAG
  TGCTAAAGCTTTATGCCTGGGAGCTGGCATTCAAGGACAAGGTGCTGGCCATCAGGCAGGAGGAGCTGAA
  GGTGCTGAAGAAGTCTGCCTACCTGTCAGCCGTGGGCACCTTCACCTGGGTCTGCACGCCCTTTCTGGTG
  GCCCTGTGCACATTTGCCGTCTACGTGACCATTGACGAGAACAACATCCTGGATGCCCAGACAGCCTTCG
  TGTCTTTGGCCTTGTTCAACATCCTCCGGTTTCCCCTGAACATTCTCCCCATGGTCATCAGCAGCATCGT
  GCAGGCGAGTGTCTCCCTCAAACGCCTGAGGATCTTTCTCTCCCATGAGGAGCTGGAACCTGACAGCATC
  GAGCGACGGCCTGTCAAAGACGGCGGGGGCACGAACAGCATCACCGTGAGGAATGCCACATTCACCTGGG
  CCAGGAGCGACCCTCCCACACTGAATGGCATCACCTTCTCCATCCCCGAAGGTGCTTTGGTGGCCGTGGT
  GGGCCAGGTGGGCTGCGGAAAGTCGTCCCTGCTCTCAGCCCTCTTGGCTGAGATGGACAAAGTGGAGGGG
  CACGTGGCTATCAAGGGCTCCGTGGCCTATGTGCCACAGCAGGCCTGGATTCAGAATGATTCTCTCCGAG
  AAAACATCCTTTTTGGATGTCAGCTGGAGGAACCATATTACAGGTCCGTGATACAGGCCTGTGCCCTCCT
  CCCAGACCTGGAAATCCTGCCCAGTGGGGATCGGACAGAGATTGGCGAGAAGGGCGTGAACCTGTCTGGG
  GGCCAGAAGCAGCGCGTGAGCCTGGCCCGGGCCGTGTACTCCAACGCTGACATTTACCTCTTCGATGATC
  CCCTCTCAGCAGTGGATGCCCATGTGGGAAAACACATCTTTGAAAATGTGATTGGCCCCAAGGGGATGCT
  GAAGAACAAGACGCGGATCTTGGTCACGCACAGCATGAGCTACTTGCCGCAGGTGGACGTCATCATCGTC
  ATGAGTGGCGGCAAGATCTCTGAGATGGGCTCCTACCAGGAGCTGCTGGCTCGAGACGGCGCCTTCGCTG
  AGTTCCTGCGTACCTATGCCAGCACAGAGCAGGAGCAGGATGCAGAGGAGAACGGGGTCACGGGCGTCAG
  CGGTCCAGGGAAGGAAGCAAAGCAAATGGAGAATGGCATGCTGGTGACGGACAGTGCAGGGAAGCAACTG
  CAGAGACAGCTCAGCAGCTCCTCCTCCTATAGTGGGGACATCAGCAGGCACCACAACAGCACCGCAGAAC
  TGCAGAAAGCTGAGGCCAAGAAGGAGGAGACCTGGAAGCTGATGGAGGCTGACAAGGCGCAGACAGGGCA
  GGTCAAGCTTTCCGTGTACTGGGACTACATGAAGGCCATCGGACTCTTCATCTCCTTCCTCAGCATCTTC
  CTTTTCATGTGTAACCATGTGTCCGCGCTGGCTTCCAACTATTGGCTCAGCCTCTGGACTGATGACCCCA
  TCGTCAACGGGACTCAGGAGCACACGAAAGTCCGGCTGAGCGTCTATGGAGCCCTGGGCATTTCACAAGG
  GATCGCCGTGTTTGGCTACTCCATGGCCGTGTCCATCGGGGGGATCTTGGCTTCCCGCTGTCTGCACGTG
  GACCTGCTGCACAGCATCCTGCGGTCACCCATGAGCTTCTTTGAGCGGACCCCCAGTGGGAACCTGGTGA
  ACCGCTTCTCCAAGGAGCTGGACACAGTGGACTCCATGATCCCGGAGGTCATCAAGATGTTCATGGGCTC
  CCTGTTCAACGTCATTGGTGCCTGCATCGTTATCCTGCTGGCCACGCCCATCGCCGCCATCATCATCCCG
  CCCCTTGGCCTCATCTACTTCTTCGTCCAGAGGTTCTACGTGGCTTCCTCCCGGCAGCTGAAGCGCCTCG
  AGTCGGTCAGCCGCTCCCCGGTCTATTCCCATTTCAACGAGACCTTGCTGGGGGTCAGCGTCATTCGAGC
  CTTCGAGGAGCAGGAGCGCTTCATCCACCAGAGTGACCTGAAGGTGGACGAGAACCAGAAGGCCTATTAC
  CCCAGCATCGTGGCCAACAGGTGGCTGGCCGTGCGGCTGGAGTGTGTGGGCAACTGCATCGTTCTGTTTG
  CTGCCCTGTTTGCGGTGATCTCCAGGCACAGCCTCAGTGCTGGCTTGGTGGGCCTCTCAGTGTCTTACTC
  ATTGCAGGTCACCACGTACTTGAACTGGCTGGTTCGGATGTCATCTGAAATGGAAACCAACATCGTGGCC
  GTGGAGAGGCTCAAGGAGTATTCAGAGACTGAGAAGGAGGCGCCCTGGCAAATCCAGGAGACAGCTCCGC
  CCAGCAGCTGGCCCCAGGTGGGCCGAGTGGAATTCCGGAACTACTGCCTGCGCTACCGAGAGGACCTGGA
  CTTCGTTCTCAGGCACATCAATGTCACGATCAATGGGGGAGAAAAGGTCGGCATCGTGGGGCGGACGGGA
  GCTGGGAAGTCGTCCCTGACCCTGGGCTTATTTCGGATCAACGAGTCTGCCGAAGGAGAGATCATCATCG
  ATGGCATCAACATCGCCAAGATCGGCCTGCACGACCTCCGCTTCAAGATCACCATCATCCCCCAGGACCC
  TGTTTTGTTTTCGGGTTCCCTCCGAATGAACCTGGACCCATTCAGCCAGTACTCGGATGAAGAAGTCTGG
  ACGTCCCTGGAGCTGGCCCACCTGAAGGACTTCGTGTCAGCCCTTCCTGACAAGCTAGACCATGAATGTG
  CAGAAGGCGGGGAGAACCTCAGTGTCGGGCAGCGCCAGCTTGTGTGCCTAGCCCGGGCCCTGCTGAGGAA
  GACGAAGATCCTTGTGTTGGATGAGGCCACGGCAGCCGTGGACCTGGAAACGGACGACCTCATCCAGTCC
  ACCATCCGGACACAGTTCGAGGACTGCACCGTCCTCACCATCGCCCACCGGCTCAACACCATCATGGACT
  ACACAAGGGTGATCGTCTTGGACAAAGGAGAAATCCAGGAGTACGGCGCCCCATCGGACCTCCTGCAGCA
  GAGAGGTCTTTTCTACAGCATGGCCAAAGACGCCGGCTTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218182 representing NM_004996
  Red=Cloning site Green=Tags(s)
  
  MALRGFCSADGSDPLWDWNVTWNTSNPDFTKCFQNTVLVWVPCFYLWACFPFYFLYLSRHDRGYIQMTPL
  NKTKTALGFLLWIVCWADLFYSFWERSRGIFLAPVFLVSPTLLGITMLLATFLIQLERRKGVQSSGIMLT
  FWLVALVCALAILRSKIMTALKEDAQVDLFRDITFYVYFSLLLIQLVLSCFSDRSPLFSETIHDPNPCPE
  SSASFLSRITFWWITGLIVRGYRQPLEGSDLWSLNKEDTSEQVVPVLVKNWKKECAKTRKQPVKVVYSSK
  DPAQPKESSKVDANEEVEALIVKSPQKEWNPSLFKVLYKTFGPYFLMSFFFKAIHDLMMFSGPQILKLLI
  KFVNDTKAPDWQGYFYTVLLFVTACLQTLVLHQYFHICFVSGMRIKTAVIGAVYRKALVITNSARKSSTV
  GEIVNLMSVDAQRFMDLATYINMIWSAPLQVILALYLLWLNLGPSVLAGVAVMVLMVPVNAVMAMKTKTY
  QVAHMKSKDNRIKLMNEILNGIKVLKLYAWELAFKDKVLAIRQEELKVLKKSAYLSAVGTFTWVCTPFLV
  ALCTFAVYVTIDENNILDAQTAFVSLALFNILRFPLNILPMVISSIVQASVSLKRLRIFLSHEELEPDSI
  ERRPVKDGGGTNSITVRNATFTWARSDPPTLNGITFSIPEGALVAVVGQVGCGKSSLLSALLAEMDKVEG
  HVAIKGSVAYVPQQAWIQNDSLRENILFGCQLEEPYYRSVIQACALLPDLEILPSGDRTEIGEKGVNLSG
  GQKQRVSLARAVYSNADIYLFDDPLSAVDAHVGKHIFENVIGPKGMLKNKTRILVTHSMSYLPQVDVIIV
  MSGGKISEMGSYQELLARDGAFAEFLRTYASTEQEQDAEENGVTGVSGPGKEAKQMENGMLVTDSAGKQL
  QRQLSSSSSYSGDISRHHNSTAELQKAEAKKEETWKLMEADKAQTGQVKLSVYWDYMKAIGLFISFLSIF
  LFMCNHVSALASNYWLSLWTDDPIVNGTQEHTKVRLSVYGALGISQGIAVFGYSMAVSIGGILASRCLHV
  DLLHSILRSPMSFFERTPSGNLVNRFSKELDTVDSMIPEVIKMFMGSLFNVIGACIVILLATPIAAIIIP
  PLGLIYFFVQRFYVASSRQLKRLESVSRSPVYSHFNETLLGVSVIRAFEEQERFIHQSDLKVDENQKAYY
  PSIVANRWLAVRLECVGNCIVLFAALFAVISRHSLSAGLVGLSVSYSLQVTTYLNWLVRMSSEMETNIVA
  VERLKEYSETEKEAPWQIQETAPPSSWPQVGRVEFRNYCLRYREDLDFVLRHINVTINGGEKVGIVGRTG
  AGKSSLTLGLFRINESAEGEIIIDGINIAKIGLHDLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEVW
  TSLELAHLKDFVSALPDKLDHECAEGGENLSVGQRQLVCLARALLRKTKILVLDEATAAVDLETDDLIQS
  TIRTQFEDCTVLTIAHRLNTIMDYTRVIVLDKGEIQEYGAPSDLLQQRGLFYSMAKDAGLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004996
• ORF Size: 4593 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004996.1, NM_004996.2, NM_004996.3, NP_004987.1
• RefSeq Size: 5927 bp
• RefSeq ORF: 4596 bp
• Locus ID: 4363
• Cytogenetics: 16p13.11
• Domains: ABC_membrane, ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 171.4 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra-and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a multispecific organic anion transporter, with oxidized glutatione, cysteinyl leukotrienes, and activated aflatoxin B1 as substrates. This protein also transports glucuronides and sulfate conjugates of steroid hormones and bile salts. Alternatively spliced variants of this gene have been described but their full-length nature is unknown. [provided by RefSeq, Apr 2012]'