Nestin (NES) (NM_006617) Human Tagged ORF Clone

CAT#: RC218197

NES (Myc-DDK-tagged)-Human nestin (NES)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NES (RC218197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NES (1:500).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NES (Cat# RC218197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NES (Cat# TA809210)(1:2000).

• 

  Coomassie blue staining of purified NES protein (Cat# TP318197). The protein was produced from HEK293T cells transfected with NES cDNA clone (Cat# RC218197) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NES
• Synonyms: Nbla00170
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218197 representing NM_006617.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGGGCTGCATGGGGGAGGAGTCGTTTCAGATGTGGGAGCTCAATCGGCGCCTGGAGGCCTACCTG
  GCCCGGGTCAAGGCGCTGGAGGAGCAGAATGAGCTGCTCAGCGCGGAGCTCGGGGGGCTCCGGGCACAA
  TCCGCGGACACCTCCTGGCGGGCGCATGCCGACGACGAGCTGGCGGCCCTGCGGGCCCTCGTTGACCAA
  CGCTGGCGGGAGAAGCACGCGGCCGAGGTGGCGCGCGACAACCTGGCTGAAGAGCTGGAGGGCGTGGCA
  GGCCGATGCCAGCAGCTGCGGCTGGCCCGGGAGCGGACGACGGAGGAGGTAGCCCGCAACCGGCGCGCC
  GTCGAGGCAGAGAAATGCGCCCGGGCCTGGCTGAGTAGCCAGGTGGCAGAGCTGGAGCGCGAGCTAGAG
  GCTCTACGCGTGGCGCACGAGGAGGAGCGCGTCGGCCTGAACGCGCAGGCTGCCTGTGCCCCCCGCTGC
  CCCGCGCCGCCCCGCGGGCCTCCCGCGCCGGCCCCGGAGGTAGAGGAGCTGGCAAGGCGACTGGGCGAG
  GCGTGGCGCGGGGCAGTGCGCGGCTACCAGGAGCGCGTGGCACACATGGAGACGTCGCTGGGCCAGGCC
  CGCGAGCGGCTGGGCCGGGCGGTGCAGGGTGCCCGCGAGGGCCGCCTGGAGCTGCAGCAGCTCCAGGCT
  GAGCGCGGAGGCCTCCTGGAGCGCAGGGCAGCGTTGGAACAGAGGTTGGAGGGCCGCTGGCAGGAGCGG
  CTGCGGGCTACTGAAAAGTTCCAGCTGGCTGTGGAGGCCCTGGAGCAGGAGAAACAGGGCCTACAGAGC
  CAGATCGCTCAGGTCCTGGAAGGTCGGCAGCAGCTGGCGCACCTCAAGATGTCCCTCAGCCTGGAGGTG
  GCCACGTACAGGACCCTCCTGGAGGCTGAGAACTCCCGGCTGCAAACACCTGGCGGTGGCTCCAAGACT
  TCCCTCAGCTTTCAGGACCCCAAGCTGGAGCTGCAATTCCCTAGGACCCCAGAGGGCCGGCGTCTTGGA
  TCTTTGCTCCCAGTCCTGAGCCCAACTTCCCTCCCCTCACCCTTGCCTGCTACCCTTGAGACACCTGTG
  CCAGCCTTTCTTAAGAACCAAGAATTCCTCCAGGCCCGTACCCCTACCTTGGCCAGCACCCCCATCCCC
  CCCACACCTCAGGCACCCTCTCCTGCTGTAGATGCAGAGATCAGAGCCCAGGATGCTCCTCTCTCTCTG
  CTCCAGACACAGGGTGGGAGGAAACAGGCTCCAGAGCCCCTGCGGGCTGAAGCCAGGGTGGCCATTCCT
  GCCAGCGTCCTGCCTGGACCAGAGGAGCCTGGGGGCCAGCGGCAAGAGGCCAGTACAGGCCAGTCCCCA
  GAGGACCATGCCTCCTTGGCACCACCCCTCAGCCCTGACCACTCCAGTTTAGAGGCTAAGGATGGAGAA
  TCCGGTGGGTCTAGAGTGTTCAGCATATGCCGAGGGGAAGGTGAAGGGCAAATCTGGGGGTTGGTAGAG
  AAAGAAACAGCCATAGAGGGCAAAGTGGTAAGCAGCTTGCAGCAGGAAATATGGGAAGAAGAGGATCTA
  AACAGGAAGGAAATCCAGGACTCCCAGGTTCCTTTGGAAAAAGAAACCCTGAAGTCTCTGGGAGAGGAG
  ATTCAAGAGTCACTGAAGACTCTGGAAAACCAGAGCCATGAGACACTAGAAAGGGAGAATCAAGAATGT
  CCGAGGTCTTTAGAAGAAGACTTAGAAACACTAAAAAGTCTAGAAAAGGAAAATAAAGAGCTATTAAAG
  GATGTGGAGGTAGTGAGACCTCTAGAAAAAGAGGCTGTAGGCCAACTTAAGCCTACAGGAAAAGAGGAC
  ACACAGACATTGCAATCCCTGCAAAAGGAGAATCAAGAACTAATGAAATCTCTTGAAGGTAATCTAGAG
  ACATTTTTATTTCCAGGAACGGAAAATCAAGAATTAGTAAGTTCTCTGCAAGAGAACTTAGAGTCATTG
  ACAGCTCTGGAAAAGGAGAATCAAGAGCCACTGAGATCTCCAGAAGTAGGGGATGAGGAGGCACTGAGA
  CCTCTGACAAAGGAGAATCAGGAACCCCTGAGGTCTCTTGAAGATGAGAACAAAGAGGCCTTTAGATCT
  CTAGAAAAAGAGAACCAGGAGCCACTGAAGACTCTAGAAGAAGAGGACCAGAGTATTGTGAGACCTCTA
  GAAACAGAGAATCACAAATCACTGAGGTCTTTAGAAGAACAGGACCAAGAGACATTGAGAACTCTTGAA
  AAAGAGACTCAACAGCGACGGAGGTCTCTAGGGGAACAGGATCAGATGACATTAAGACCCCCAGAAAAA
  GTGGATCTAGAACCACTGAAGTCTCTTGACCAGGAGATAGCTAGACCTCTTGAAAATGAGAATCAAGAG
  TTCTTAAAGTCACTCAAAGAAGAGAGCGTAGAGGCAGTAAAATCTTTAGAAACAGAGATCCTAGAATCA
  CTGAAGTCTGCGGGACAAGAGAACCTGGAAACACTGAAATCTCCAGAAACTCAAGCACCACTGTGGACT
  CCAGAAGAAATAAATCAGGGGGCAATGAATCCTCTAGAAAAGGAAATTCAAGAACCACTGGAGTCTGTG
  GAAGTGAACCAAGAGACATTCAGACTCCTGGAAGAGGAGAATCAGGAATCATTGAGATCTCTGGGAGCA
  TGGAACCTGGAGAATTTGAGATCTCCAGAGGAGGTAGACAAGGAAAGTCAAAGGAATCTGGAAGAGGAA
  GAGAACCTGGGAAAGGGAGAGTACCAAGAGTCACTGAGGTCTCTGGAGGAGGAGGGACAGGAGCTGCCG
  CAGTCTGCAGATGTGCAGAGGTGGGAAGATACGGTGGAGAAGGACCAAGAACTGGCTCAGGAAAGCCCT
  CCTGGGATGGCTGGAGTGGAAAATGAGGATGAGGCAGAGCTGAATCTGAGGGAGCAGGATGGCTTCACT
  GGGAAGGAGGAGGTGGTAGAGCAGGGAGAGCTGAATGCCACAGAGGAGGTCTGGATCCCAGGCGAGGGG
  CACCCAGAGAGCCCTGAGCCCAAAGAGCAGAGAGGCCTGGTTGAGGGAGCCAGTGTGAAGGGAGGGGCT
  GAGGGCCTCCAGGACCCTGAAGGGCAATCACAACAGGTGGGGGCCCCAGGCCTCCAGGCTCCCCAGGGG
  CTGCCAGAGGCGATAGAGCCCCTGGTGGAAGATGATGTGGCCCCAGGGGGTGACCAAGCCTCCCCAGAG
  GTCATGTTGGGGTCAGAGCCTGCCATGGGTGAGTCTGCTGCGGGAGCTGAGCCAGGCCCGGGGCAGGGG
  GTGGGAGGGCTGGGGGACCCAGGCCATCTGACCAGGGAAGAGGTGATGGAACCACCCCTGGAAGAGGAG
  AGTTTGGAGGCAAAGAGGGTTCAGGGCTTGGAAGGGCCTAGAAAGGACCTAGAGGAGGCAGGTGGTCTG
  GGGACAGAGTTCTCCGAGCTGCCTGGGAAGAGCAGAGACCCTTGGGAGCCTCCCAGGGAGGGTAGGGAG
  GAGTCAGAGGCTGAGGCCCCCAGGGGAGCAGAGGAGGCGTTCCCTGCTGAGACCCTGGGCCACACTGGA
  AGTGATGCCCCTTCACCTTGGCCTCTGGGGTCAGAGGAAGCTGAGGAGGATGTACCACCAGTGCTGGTC
  TCCCCCAGCCCAACGTACACCCCGATCCTGGAAGATGCCCCTGGGCCTCAGCCTCAGGCTGAAGGGAGT
  CAGGAGGCTAGCTGGGGGGTGCAGGGGAGGGCTGAAGCCCTGGGGAAAGTAGAGAGCGAGCAGGAGGAG
  TTGGGTTCTGGGGAGATCCCCGAGGGCCCCCAGGAGGAAGGGGAGGAGAGCAGAGAAGAGAGCGAGGAG
  GATGAGCTCGGGGAGACCCTTCCAGACTCCACTCCCCTGGGCTTCTACCTCAGGTCCCCCACCTCCCCC
  AGGTGGGACCCCACTGGAGAGCAGAGGCCACCCCCTCAAGGGGAGACTGGAAAGGAGGGCTGGGATCCT
  GCTGTCCTGGCTTCCGAGGGCCTTGAGGCCCCACCCTCAGAAAAGGAGGAGGGGGAGGAGGGAGAAGAG
  GAGTGTGGCCGTGACTCTGACCTGTCAGAAGAATTTGAGGACCTGGGGACTGAGGCACCTTTTCTTCCT
  GGGGTCCCTGGGGAGGTGGCAGAACCTCTGGGCCAGGTGCCCCAGCTGCTACTGGATCCTGCAGCCTGG
  GATCGAGATGGGGAGTCCGATGGGTTTGCAGATGAGGAAGAAAGTGGGGAGGAGGGAGAGGAGGATCAG
  GAGGAGGGGAGGGAGCCAGGGGCTGGGCGGTGGGGGCCAGGGTCTTCTGTTGGCAGCCTCCAGGCCCTG
  AGTAGCTCCCAGAGAGGGGAATTCCTGGAGTCTGATTCTGTGAGTGTCAGTGTCCCCTGGGATGACAGC
  TTGAGGGGTGCAGTGGCTGGTGCCCCCAAGACTGCCCTGGAAACGGAGTCCCAGGACAGTGCTGAGCCT
  TCTGGCTCAGAGGAAGAGTCTGACCCTGTTTCCTTGGAGAGGGAGGACAAAGTCCCTGGCCCTCTAGAG
  ATCCCCAGTGGGATGGAGGATGCAGGCCCAGGGGCAGACATCATTGGTGTTAATGGCCAGGGTCCCAAC
  TTGGAGGGGAAGTCACAGCATGTGAATGGGGGAGTGATGAACGGGCTGGAGCAGTCTGAGGAAGTGGGG
  CAAGGAATGCCGCTAGTCTCTGAGGGAGACCGAGGGAGCCCCTTTCAGGAGGAGGAGGGGAGTGCTCTG
  AAGACCTCTTGGGCAGGGGCTCCTGTTCACCTGGGCCAGGGTCAGTTCCTGAAGTTCACTCAGAGGGAA
  GGAGATAGAGAGTCCTGGTCCTCAGGGGAGGAC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC218197
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEGCMGEESFQMWELNRRLEAYLARVKALEEQNELLSAELGGLRAQSADTSWRAHADDELAALRALVDQ
  RWREKHAAEVARDNLAEELEGVAGRCQQLRLARERTTEEVARNRRAVEAEKCARAWLSSQVAELERELE
  ALRVAHEEERVGLNAQAACAPRCPAPPRGPPAPAPEVEELARRLGEAWRGAVRGYQERVAHMETSLGQA
  RERLGRAVQGAREGRLELQQLQAERGGLLERRAALEQRLEGRWQERLRATEKFQLAVEALEQEKQGLQS
  QIAQVLEGRQQLAHLKMSLSLEVATYRTLLEAENSRLQTPGGGSKTSLSFQDPKLELQFPRTPEGRRLG
  SLLPVLSPTSLPSPLPATLETPVPAFLKNQEFLQARTPTLASTPIPPTPQAPSPAVDAEIRAQDAPLSL
  LQTQGGRKQAPEPLRAEARVAIPASVLPGPEEPGGQRQEASTGQSPEDHASLAPPLSPDHSSLEAKDGE
  SGGSRVFSICRGEGEGQIWGLVEKETAIEGKVVSSLQQEIWEEEDLNRKEIQDSQVPLEKETLKSLGEE
  IQESLKTLENQSHETLERENQECPRSLEEDLETLKSLEKENKELLKDVEVVRPLEKEAVGQLKPTGKED
  TQTLQSLQKENQELMKSLEGNLETFLFPGTENQELVSSLQENLESLTALEKENQEPLRSPEVGDEEALR
  PLTKENQEPLRSLEDENKEAFRSLEKENQEPLKTLEEEDQSIVRPLETENHKSLRSLEEQDQETLRTLE
  KETQQRRRSLGEQDQMTLRPPEKVDLEPLKSLDQEIARPLENENQEFLKSLKEESVEAVKSLETEILES
  LKSAGQENLETLKSPETQAPLWTPEEINQGAMNPLEKEIQEPLESVEVNQETFRLLEEENQESLRSLGA
  WNLENLRSPEEVDKESQRNLEEEENLGKGEYQESLRSLEEEGQELPQSADVQRWEDTVEKDQELAQESP
  PGMAGVENEDEAELNLREQDGFTGKEEVVEQGELNATEEVWIPGEGHPESPEPKEQRGLVEGASVKGGA
  EGLQDPEGQSQQVGAPGLQAPQGLPEAIEPLVEDDVAPGGDQASPEVMLGSEPAMGESAAGAEPGPGQG
  VGGLGDPGHLTREEVMEPPLEEESLEAKRVQGLEGPRKDLEEAGGLGTEFSELPGKSRDPWEPPREGRE
  ESEAEAPRGAEEAFPAETLGHTGSDAPSPWPLGSEEAEEDVPPVLVSPSPTYTPILEDAPGPQPQAEGS
  QEASWGVQGRAEALGKVESEQEELGSGEIPEGPQEEGEESREESEEDELGETLPDSTPLGFYLRSPTSP
  RWDPTGEQRPPPQGETGKEGWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLP
  GVPGEVAEPLGQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQAL
  SSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLE
  IPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPLVSEGDRGSPFQEEEGSAL
  KTSWAGAPVHLGQGQFLKFTQREGDRESWSSGED
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC218197 also available, TP318197M
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006617
• ORF Size: 4863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006617.2
• RefSeq Size: 5591 bp
• RefSeq ORF: 4866 bp
• Locus ID: 10763
• UniProt ID: P48681
• Cytogenetics: 1q23.1
• Protein Families: ES Cell Differentiation/IPS
• MW: 177.4 kDa
• Gene Summary: This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]