Nucleostemin (GNL3) (NM_206825) Human Tagged ORF Clone

CAT#: RC218355

GNL3 (Myc-DDK-tagged)-Human guanine nucleotide binding protein-like 3 (nucleolar) (GNL3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GNL3
• Synonyms: C77032; E2IG3; NNP47; NS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218355 representing NM_206825
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTGCCATAAGCGGTATAAAATCCAAAAAAAGGTTCGAGAACATCATCGAAAATTAAGAAAGGAGG
  CTAAAAAGCGGGGTCACAAGAAGCCTAGGAAAGACCCAGGAGTTCCAAACAGTGCTCCCTTTAAGGAGGC
  TCTTCTTAGGGAAGCTGAGCTAAGGAAACAGAGGCTTGAAGAACTAAAACAGCAGCAGAAACTTGACAGG
  CAGAAGGAACTAGAAAAGAAAAGAAAACTTGAAACTAATCCTGATATTAAGCCATCAAATGTGGAACCTA
  TGGAAAAGGAGTTTGGGCTTTGCAAAACTGAGAACAAAGCCAAGTCGGGCAAACAGAATTCAAAGAAGCT
  GTACTGCCAAGAACTTAAAAAGGTGATTGAAGCCTCCGATGTTGTCCTAGAGGTGTTGGATGCCAGAGAT
  CCTCTTGGTTGCAGATGTCCTCAGGTAGAAGAGGCCATTGTCCAGAGTGGACAGAAAAAGCTGGTACTTA
  TATTAAATAAATCAGATCTGGTACCAAAGGAGAATTTGGAGAGCTGGCTAAATTATTTGAAGAAAGAATT
  GCCAACAGTGGTGTTCAGAGCCTCAACAAAACCAAAGGATAAAGGGAAGATAACCAAGCGTGTGAAGGCA
  AAGAAGAATGCTGCTCCATTCAGAAGTGAAGTCTGCTTTGGGAAAGAGGGCCTTTGGAAACTTCTTGGAG
  GTTTTCAGGAAACTTGCAGCAAAGCCATTCGGGTTGGAGTAATTGGTTTCCCAAATGTGGGGAAAAGCAG
  CATTATCAATAGCTTAAAACAAGAACAGATGTGTAATGTTGGTGTATCCATGGGGCTTACAAGGAGCATG
  CAAGTTGTCCCCTTGGACAAACAGATCACAATCATAGATAGTCCGAGCTTCATCGTATCTCCACTTAATT
  CCTCCTCTGCGCTTGCTCTGCGAAGTCCAGCAAGTATTGAAGTAGTAAAACCGATGGAGGCTGCCAGTGC
  CATCCTTTCCCAGGCTGATGCTCGACAGGTAGTACTGAAATATACTGTCCCAGGCTACAGGAATTCTCTG
  GAATTTTTTACTGTGCTTGCTCAGAGAAGAGGTATGCACCAAAAAGGTGGAATCCCAAATGTTGAAGGTG
  CTGCCAAACTGCTGTGGTCTGAGTGGACAGGTGCCTCATTAGCTTACTATTGCCATCCCCCTACATCTTG
  GACTCCTCCTCCATATTTTAATGAGAGTATTGTGGTAGACATGAAAAGCGGCTTCAATCTGGAAGAACTG
  GAAAAGAACAATGCACAGAGCATAAGAGCCATCAAGGGCCCTCATTTGGCCAATAGCATCCTTTTCCAGT
  CTTCCGGTCTGACAAATGGAATAATAGAAGAAAAGGACATACATGAAGAATTGCCAAAACGGAAAGAAAG
  GAAGCAGGAGGAGAGGGAGGATGACAAAGACAGTGACCAGGAAACTGTTGATGAAGAAGTTGATGAAAAC
  AGCTCAGGCATGTTTGCTGCAGAAGAGACAGGGGAGGCACTGTCTGAGGAGACTACAGCAGGTGAACAGT
  CTACAAGGTCTTTTATCTTGGATAAAATCATTGAAGAGGATGATGCTTATGACTTCAGTACAGATTATGT
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218355 representing NM_206825
  Red=Cloning site Green=Tags(s)
  
  MTCHKRYKIQKKVREHHRKLRKEAKKRGHKKPRKDPGVPNSAPFKEALLREAELRKQRLEELKQQQKLDR
  QKELEKKRKLETNPDIKPSNVEPMEKEFGLCKTENKAKSGKQNSKKLYCQELKKVIEASDVVLEVLDARD
  PLGCRCPQVEEAIVQSGQKKLVLILNKSDLVPKENLESWLNYLKKELPTVVFRASTKPKDKGKITKRVKA
  KKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSM
  QVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSL
  EFFTVLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEEL
  EKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDEN
  SSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_206825
• ORF Size: 1611 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_206825.1, NP_996561.1
• RefSeq Size: 2159
• RefSeq ORF: 1614
• Locus ID: 26354
• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency
• MW: 60.4 kDa
• Gene Summary: The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]