ZNF750 (NM_024702) Human Tagged ORF Clone

CAT#: RC218356

ZNF750 (Myc-DDK-tagged)-Human zinc finger protein 750 (ZNF750)

Product Images

Western blot validation of overexpression lysate (Cat# LY411159) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218356 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF750
• Synonyms: ZFP750
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218356 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCTCCTCAAAGAGCGGAAGCCAAAAAAGCCACATTACATCCCCAGGCCTCCAGGAAAGCCCTTCA
  AGTATAAATGTTTCCAATGTCCCTTTACTTGCAATGAGAAGTCACATCTTTTTAATCACATGAAGTATGG
  TCTTTGTAAAAACTCGATTACTTTAGTATCAGAGCAGGATCGAGTTCCCAAGTGCCCTAAATCTAACTCA
  CTAGACCCCAAGCAAACCAACCAGCCCGATGCCACGGCGAAGCCAGCCTCTTCCAAGTCTGTCGCAAATG
  GACTCTCTGCCTTCGACTCGAAGCTTCAGCACAGCTCTGCCAGGGAAGACATCAAGGAAAACCTGGAGCT
  GCAAGCCCGGGGAACCCACAGGTGCCTGGGACAGAAGCCAGCCCTCCACAGGGCATCACCCTGCAAGAGC
  CCAGCTCCGGAAGCCGCCCTCGGTGCCCAGCCTGCTCTGGAAGGCGCAGCTCGGCCTTCTGCATTTGTTC
  CAGTCGGCGAGCACAGACTCAAGGGGCCAGACAACGCCGAGGCGCCCGAGACACTGGCTTTACACAACCC
  CACTGCCAAGGCCGTGTCTTTCCACACCAAGTCGGCCTTCCACACTCCTGGCTACCCCTGGAAAGCCGGC
  TCACCTTTCCTTCCACCAGAGTTTCCACATAAAATCTCATCTACAAAGGGGCTTGGGGCCATTTCCCCTT
  ACATGCACCCCACAATCCCAGAGTACCCGCCTCACTTTTACACAGAGCACGGGCTGGCCACCATCTACTC
  GCCTTACCTGCTGGCTGGGAGCTCGCCTGAGTGTGACGCACCCCTGCTGTCAGTCTACGGAACCCAAGAC
  CCGAGACACTTCCTGCCTCACCCGGGGCCGATCCCTAAGCACCTGGCTCCATCTCCAGCCACATACGATC
  ACTACAGGTTTTTCCAGCAATATCCCTCTAACCTGCCGATTCCTTACGGATTTTACAGGCCAGAGTCTGC
  ATTTTCCTCCTATGGTCTCAGACTCCCACCTGTCACTGGCCTCACCCGAGATCAGAGCTCTCACCTGCTT
  GAAGAAGCCACCCTGGTCTATCCAGCCTCGAGTCCTTCCAGGTTAAACCCTTCGGACCCCAACAGAAAAC
  ACGTCGAGTTCGAAAGTCCAATTCCTGAGGCTAAAGACTCCTCCAAGGCTGGGCAGAGAGACACGGAAGG
  GTCCAAAATGAGCCCCCGCGCAGGGAGTGCAGCCACGGGCTCCCCAGGGAGGCCGAGCCCCACCGACTTC
  ATGCAGACGAGCCAGACCTGCGAAGGCCTGTACGACCTCTCCAACAAGGCAGCCTCCAGCGCACTGGGAA
  GACTCTACCCGCCAGAGCAAAGCCTCACAGCCTTCAGGCCTGTTAAGAAAAGCACAGAATGCCTACCTGC
  CCAGGCTGCTGAGACCACAGCAGAGTCTCCAGTAAGCCTCAATGTTGTGAACGGAGACCCTCCTGCTCCG
  ACCGGAAGCGCCTCTCTCGTCTCGGAGGCCGCGCCTTCCAGTCCGGACGACAGCTCCGGGATGGGCCCCC
  TCAACCTCTCCAAGAAATCAGAGATAAACCTGGCAGCCACCCACGAACCCACGTACCAAGGCAGCCCCCA
  GGCGGAAACCGCCAGCTTCTCAGAGCTGCAGGACCTTCCACTCAATCTCTCGGTGAAGGACCCCTGTAAC
  ACCCAGGCTCCGAGGCCCGCCTTCCCCGGTCGACCACGAGCTGCGGAACCTGCTGCTGCTGTTCCACAGA
  AGACTGGGACAGAAGGTTCTGAGGATGGGCCCAGCCACCCTGAGACCAAGCCAGGCAGCCTCGACGGTGA
  CGGGGCCCCACCCACAGGCCCCGGCGAGGAGGCTCCAGACGCATGCGCGGTGGACAGCAGCGAGGAGCAG
  AAGCAGACGGCAGCCGTGGCCCTGTGCCAGCTGGCGGCCTACAGCCCCAGGAACATCCGGGTGGGCGATG
  GGGATGCTGCGGCCCCGGAACCTGCCTGCCGGCAAGACACACCCACACTGAGCTCCATGGAGAGCCAAGA
  GGCCCAGTGTGACCTCAGACCCAAAGGACAAAAGAGGACAAGTCTAAGGGATGCTGGAAAATCCCAGCAA
  GGAGCTAAGAAGGCGAAGCTGCAGGACACGGCCAGAGTGTTCACACTACGAAGGAGGGCCCGGGTGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218356 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLLKERKPKKPHYIPRPPGKPFKYKCFQCPFTCNEKSHLFNHMKYGLCKNSITLVSEQDRVPKCPKSNS
  LDPKQTNQPDATAKPASSKSVANGLSAFDSKLQHSSAREDIKENLELQARGTHRCLGQKPALHRASPCKS
  PAPEAALGAQPALEGAARPSAFVPVGEHRLKGPDNAEAPETLALHNPTAKAVSFHTKSAFHTPGYPWKAG
  SPFLPPEFPHKISSTKGLGAISPYMHPTIPEYPPHFYTEHGLATIYSPYLLAGSSPECDAPLLSVYGTQD
  PRHFLPHPGPIPKHLAPSPATYDHYRFFQQYPSNLPIPYGFYRPESAFSSYGLRLPPVTGLTRDQSSHLL
  EEATLVYPASSPSRLNPSDPNRKHVEFESPIPEAKDSSKAGQRDTEGSKMSPRAGSAATGSPGRPSPTDF
  MQTSQTCEGLYDLSNKAASSALGRLYPPEQSLTAFRPVKKSTECLPAQAAETTAESPVSLNVVNGDPPAP
  TGSASLVSEAAPSSPDDSSGMGPLNLSKKSEINLAATHEPTYQGSPQAETASFSELQDLPLNLSVKDPCN
  TQAPRPAFPGRPRAAEPAAAVPQKTGTEGSEDGPSHPETKPGSLDGDGAPPTGPGEEAPDACAVDSSEEQ
  KQTAAVALCQLAAYSPRNIRVGDGDAAAPEPACRQDTPTLSSMESQEAQCDLRPKGQKRTSLRDAGKSQQ
  GAKKAKLQDTARVFTLRRRARVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024702
• ORF Size: 2169 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024702.1, NM_024702.2, NP_078978.1
• RefSeq Size: 3218 bp
• RefSeq ORF: 2172 bp
• Locus ID: 79755
• Domains: zf-C2H2
• MW: 77.4 kDa
• Gene Summary: This gene encodes a protein with a nuclear localization site and a C2H2 zinc finger domain. Mutations in this gene have been associated with seborrhea-like dermatitis with psoriasiform elements. [provided by RefSeq, Jul 2008]