IRS4 (NM_003604) Human Tagged ORF Clone

CAT#: RC218385

IRS4 (Myc-DDK-tagged)-Human insulin receptor substrate 4 (IRS4)

Product Images

Western blot validation of overexpression lysate (Cat# LY401194) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218385 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IRS4
• Synonyms: IRS-4; PY160
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218385 representing NM_003604
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGTTGCTCCTTCACTCGCGACCAAGCGACAAGAAGACTAAGAGGTGCAGCAGCGGCGGCAGCGG
  CAGCTCTAGCAGCAGTGGTGACCACCCCGCTTCTTTCCTCGGGAACCCCGACCGCACTCATTGGGACCGG
  GTCGTCTTGTCCGGGAGCCATGTGGCTCTCCACGGCCACTGGCTCCCGGTCAGACTCCGAGTCCGAAGAG
  GAGGACCTGCCCGTCGGGGAGGAAGTCTGCAAACGCGGCTACCTGCGGAAACAGAAGCATGGGCACAGGC
  GCTACTTCGTGCTCAAACTCGAGACTGCTGACGCCCCAGCTCGGCTGGAATACTACGAAAATGCCAGGAA
  GTTCCGGCACAGTGTCCGCGCCGCGGCGGCTGCAGCAGCGGCGGCCGCCTCTGGCGCCGCGATCCCCCCG
  CTCATTCCACCGCGGCGCGTGATCACCCTATACCAGTGCTTTTCCGTGAGCCAGCGAGCAGATGCAAGGT
  ACCGACACCTCATTGCTCTTTTCACCCAAGACGAATACTTCGCGATGGTGGCCGAGAACGAGTCGGAGCA
  GGAAAGCTGGTACTTGCTGCTCAGCCGCCTCATCCTCGAGAGCAAGCGCCGCCGCTGCGGCACGCTCGGC
  GCGCAGCCGGACGGAGAGCCGGCCGCGCTGGCGGCGGCAGCGGCGGCGGAGCCACCCTTCTATAAAGATG
  TGTGGCAGGTAATAGTCAAACCCAGGGGGCTGGGGCACAGAAAAGAGCTGAGCGGCGTGTTCCGGCTGTG
  TCTAACCGACGAGGAGGTCGTGTTTGTGAGGCTGAACACCGAAGTGGCCAGCGTGGTCGTCCAGCTCCTG
  AGCATCCGTCGCTGTGGACACTCGGAGCAGTATTTCTTCTTGGAAGTAGGCAGGTCCACTGTCATCGGTC
  CGGGAGAGCTCTGGATGCAGGTCGATGACTGTGTGGTTGCCCAAAACATGCATGAGCTGTTTTTGGAGAA
  GATGAGAGCCTTGTGTGCAGACGAATACAGAGCCCGCTGCCGCAGCTACAGCATCAGCATCGGCGCCCAC
  CTGTTAACCCTGCTGTCCGCTAGGAGGCACCTGGGCTTGGTGCCGCTCGAGCCGGGAGGCTGGCTCAGAA
  GGTCCCGCTTTGAGCAGTTTTGCCACCTCAGGGCCATCGGCGACGGGGAAGACGAGATGCTTTTCACCAG
  GCGCTTCGTAACACCCAGCGAGCCTGTGGCCCACTCCAGGCGAGGAAGACTGCACCTGCCCAGAGGGCGC
  AGGTCAAGGAGAGCGGTTTCAGTGCCGGCCAGCTTTTTTCGCCGCTTAGCACCCAGCCCAGCACGTCCCC
  GGCACCCTGCAGAAGCCCCGAACAATGGAGCTCGCCTGTCTTCTGAAGTGTCTGGTTCTGGCTCTGGCAA
  CTTTGGGGAGGAAGGCAATCCCCAGGGCAAAGAAGATCAGGAAGGAAGCGGAGGTGACTACATGCCTATG
  AACAATTGGGGCTCAGGAAATGGCCGGGGCTCAGGAGGTGGCCAGGGCTCAAATGGCCAAGGCTCCAGTA
  GCCATAGCTCGGGAGGAAACCAGTGTTCAGGCGAGGGACAGGGATCCCGAGGTGGTCAGGGCTCAAATGG
  CCAGGGCTCAGGAGGAAACCAGTGCTCTAGAGATGGCCAGGGCACCGCAGGTGGGCACGGTTCAGGTGGT
  GGCCAGAGACCTGGAGGTGGGCATGGCTCAGGTGGTGGCCAGGGACCTGGAGATGGCCATGGCTCAGGTG
  GTGGCAAGAACTCTGGGGGGGGCAAAGGCTCAGGAAGTGGGAAAGGATCCGATGGTGATGGTGAACGTGG
  AAAATCTCTGAAGAAAAGATCCTATTTTGGCAAATTAACTCAAAGCAAGCAACAGCAAATGCCACCACCT
  CCACCACCTCCTCCTCCACCCCCACCAGCTGGAGGAACTGGTGGAAAAGGGAAGTCTGGGGGAAGATTCA
  GACTTTATTTTTGTGTTGACAGAGGAGCCACGAAAGAATGCAAAGAAGCCAAAGAAGTGAAAGATGCAGA
  GATCCCAGAAGGTGCAGCTCGAGGTCCCCACAGAGCCAGAGCTTTTGATGAAGATGAGGATGACCCATAC
  GTGCCAATGAGGCCAGGGGTGGCCACCCCTCTTGTAAGCTCCAGTGATTATATGCCAATGGCTCCTCAAA
  ATGTCTCTGCTTCAAAAAAGCGCCACTCTCGATCCCCTTTTGAAGATTCAAGAGGGTACATGATGATGTT
  TCCCAGAGTGAGCCCACCACCTGCTCCGAGTCCTCCAAAAGCACCTGATACTAATAAAGAGGATGACTCA
  AAGGACAATGACAGTGAGAGTGACTACATGTTTATGGCTCCTGGAGCCGGTGCAATTCCAAAAAACCCCA
  GAAATCCTCAGGGTGGCTCTTCCTCCAAAAGTTGGAGCTCCTACTTCTCTCTACCAAACCCTTTTCGGAG
  CTCACCTTTGGGACAGAATGACAACAGTGAGTATGTGCCAATGTTACCTGGAAAGTTCCTGGGGAGGGGC
  CTAGACAAAGAAGTCTCCTATAACTGGGACCCCAAAGATGCAGCTTCAAAGCCTTCAGGTGAGGGATCAT
  TCTCAAAGCCTGGAGATGGGGGATCACCTTCAAAGCCTTCAGATCATGAGCCCCCAAAGAATAAAGCTAA
  GAGACCTAACCGACTTTCTTTTATTACAAAAGGATATAAAATCAAGCCAAAACCACAAAAGCCCACACAT
  GAGCAGAGAGAAGCTGACAGCTCTAGTGACTACGTCAACATGGACTTCACTAAAAGAGAGAGCAATACAC
  CAGCTCCCTCTACTCAAGGACTACCAGATTCGTGGGGCATAATTGCTGAACCCAGACAGTCAGCCTTTTC
  TAATTATGTGAATGTTGAGTTTGGAGTGCCATTTCCAAATCCAGCAAACGACCTCTCAGATCTTTTAAGA
  GCTATACCACGTGCCAACCCCTTATCTCTGGACAGTGCTAGGTGGCCACTTCCTCCCCTTCCCCTCAGTG
  CTACAGGTAGCAATGCTATTGAGGAAGAGGGTGACTACATTGAAGTAATTTTCAACTCAGCAATGACACC
  AGCCATGGCTCTTGCTGACAGTGCCATTCGCTATGATGCTGAAACAGGTCGAATCTATGTGGTCGACCCA
  TTTTCTGAGTGCTGTATGGATATTTCTCTCTCCCCCAGCCGATGTTCTGAACCACCACCTGTAGCTAGGC
  TGCTGCAGGAAGAAGAGCAGGAGAGAAGACGCCCACAAAGCCGTTCTCAAAGTTTCTTTGCAGCAGCCAG
  AGCCGCTGTCTCTGCTTTTCCAACAGACAGCCTCGAGAGAGACCTTTCCCCATCCTCAGCCCCGGCTGTC
  GCTTCGGCTGCAGAGCCGACTTTAGCCCTCAGCCAAGTTGTAGCTGCGGCCTCCGCGCTCGCCGCAGCCC
  CGGGCATCGGCGCAGCAGCCGCAGCTGCTGGATTTGACTCCGCCTCTGCCCGCTGGTTTCAACCTGTTGC
  TAATGCTGCTGATGCCGAAGCAGTAAGGGGAGCCCAAGACGTTGCCGGTGGCTCGAACCCTGGAGCCCAC
  AACCCATCTGCAAACCTTGCCAGAGGTGATAACCAGGCTGGCGGGGCTGCCGCTGCAGCTGCCGCTCCGG
  AACCACCACCTCGCAGTCGCCGGGTGCCAAGACCCCCGGAGAGAGAAGATTCTGACAACGACGACGACAC
  TCACGTGAGAATGGATTTTGCCAGACGTGATAATCAGTTCGACTCTCCCAAAAGAGGTCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218385 representing NM_003604
  Red=Cloning site Green=Tags(s)
  
  MASCSFTRDQATRRLRGAAAAAAAALAAVVTTPLLSSGTPTALIGTGSSCPGAMWLSTATGSRSDSESEE
  EDLPVGEEVCKRGYLRKQKHGHRRYFVLKLETADAPARLEYYENARKFRHSVRAAAAAAAAAASGAAIPP
  LIPPRRVITLYQCFSVSQRADARYRHLIALFTQDEYFAMVAENESEQESWYLLLSRLILESKRRRCGTLG
  AQPDGEPAALAAAAAAEPPFYKDVWQVIVKPRGLGHRKELSGVFRLCLTDEEVVFVRLNTEVASVVVQLL
  SIRRCGHSEQYFFLEVGRSTVIGPGELWMQVDDCVVAQNMHELFLEKMRALCADEYRARCRSYSISIGAH
  LLTLLSARRHLGLVPLEPGGWLRRSRFEQFCHLRAIGDGEDEMLFTRRFVTPSEPVAHSRRGRLHLPRGR
  RSRRAVSVPASFFRRLAPSPARPRHPAEAPNNGARLSSEVSGSGSGNFGEEGNPQGKEDQEGSGGDYMPM
  NNWGSGNGRGSGGGQGSNGQGSSSHSSGGNQCSGEGQGSRGGQGSNGQGSGGNQCSRDGQGTAGGHGSGG
  GQRPGGGHGSGGGQGPGDGHGSGGGKNSGGGKGSGSGKGSDGDGERGKSLKKRSYFGKLTQSKQQQMPPP
  PPPPPPPPPAGGTGGKGKSGGRFRLYFCVDRGATKECKEAKEVKDAEIPEGAARGPHRARAFDEDEDDPY
  VPMRPGVATPLVSSSDYMPMAPQNVSASKKRHSRSPFEDSRGYMMMFPRVSPPPAPSPPKAPDTNKEDDS
  KDNDSESDYMFMAPGAGAIPKNPRNPQGGSSSKSWSSYFSLPNPFRSSPLGQNDNSEYVPMLPGKFLGRG
  LDKEVSYNWDPKDAASKPSGEGSFSKPGDGGSPSKPSDHEPPKNKAKRPNRLSFITKGYKIKPKPQKPTH
  EQREADSSSDYVNMDFTKRESNTPAPSTQGLPDSWGIIAEPRQSAFSNYVNVEFGVPFPNPANDLSDLLR
  AIPRANPLSLDSARWPLPPLPLSATGSNAIEEEGDYIEVIFNSAMTPAMALADSAIRYDAETGRIYVVDP
  FSECCMDISLSPSRCSEPPPVARLLQEEEQERRRPQSRSQSFFAAARAAVSAFPTDSLERDLSPSSAPAV
  ASAAEPTLALSQVVAAASALAAAPGIGAAAAAAGFDSASARWFQPVANAADAEAVRGAQDVAGGSNPGAH
  NPSANLARGDNQAGGAAAAAAAPEPPPRSRRVPRPPEREDSDNDDDTHVRMDFARRDNQFDSPKRGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003604
• ORF Size: 3771 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003604.1, NM_003604.2, NP_003595.1
• RefSeq Size: 3939 bp
• RefSeq ORF: 3774 bp
• Locus ID: 8471
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus
• MW: 133.6 kDa
• Gene Summary: IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.. [provided by RefSeq, Jul 2008]