T (NM_003181) Human Tagged ORF Clone
CAT#: RC218530L1
- LentiORF®
Lenti ORF clone of Human T, brachyury homolog (mouse) (T), Myc-DDK-tagged
"NM_003181" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TBXT |
Synonyms | SAVA; T; TFT |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC218530).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_003181 |
ORF Size | 1308 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003181.2 |
RefSeq Size | 2518 bp |
RefSeq ORF | 1308 bp |
Locus ID | 6862 |
UniProt ID | O15178 |
Cytogenetics | 6q27 |
Protein Families | ES Cell Differentiation/IPS, Transcription Factors |
MW | 47.3 kDa |
Gene Summary | The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. [provided by RefSeq, Sep 2018] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC303281 | T (untagged)-Human T, brachyury homolog (mouse) (T) |
USD 730.00 |
|
RC218530 | T (Myc-DDK-tagged)-Human T, brachyury homolog (mouse) (T) |
USD 420.00 |
|
RG218530 | T (GFP-tagged) - Human T, brachyury homolog (mouse) (T) |
USD 460.00 |
|
RC218530L2 | Lenti ORF clone of Human T, brachyury homolog (mouse) (T), mGFP tagged |
USD 620.00 |
|
RC218530L3 | Lenti ORF clone of Human T, brachyury homolog (mouse) (T), Myc-DDK-tagged |
USD 768.00 |
|
RC218530L4 | Lenti ORF clone of Human T, brachyury homolog (mouse) (T), mGFP tagged |
USD 768.00 |
{0} Product Review(s)
Be the first one to submit a review