T (NM_003181) Human Untagged Clone

CAT#: SC303281

T (untagged)-Human T, brachyury homolog (mouse) (T)


  "NM_003181" in other vectors (6)

Reconstitution Protocol

USD 730.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TBXT
Synonyms SAVA; T; TFT
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_003181 edited
TCTCAGGTAGCGAGTCTGGGCTTCGGGGACGGCGGGGAGGGGAGCCGGACGGGAGGATGA
GCTCCCCTGGCACCGAGAGCGCGGGAAAGAGCCTGCAGTACCGAGTGGACCACCTGCTGA
GCGCCGTGGAGAATGAGCTGCAGGCGGGCAGCGAGAAGGGCGACCCCACAGAGCGCGAAC
TGCGCGTGGGCCTGGAGGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGCTCACCAATGAGA
TGATCGTGACCAAGAACGGCAGGAGGATGTTTCCGGTGCTGAAGGTGAACGTGTCTGGCC
TGGACCCCAACGCCATGTACTCCTTCCTGCTGGACTTCGTGGCGGCGGACAACCACCGCT
GGAAGTACGTGAACGGGGAATGGGTGCCGGGGGGCAAGCCGGAGCCGCAGGCGCCCAGCT
GCGTCTACATCCACCCCGACTCGCCCAACTTCGGGGCCCACTGGATGAAGGCTCCCGTCT
CCTTCAGCAAAGTCAAGCTCACCAACAAGCTCAACGGAGGGGGCCAGATCATGCTGAACT
CCTTGCATAAGTATGAGCCTCGAATCCACATAGTGAGAGTTGGGGGTCCACAGCGCATGA
TCACCAGCCACTGCTTCCCTGAGACCCAGTTCATAGCGGTGACTGCTTATCAGAACGAGG
AGATCACAGCTCTTAAAATTAAGTACAATCCATTTGCAAAAGCTTTCCTTGATGCAAAGG
AAAGAAGTGATCACAAAGAGATGATGGAGGAACCCGGAGACAGCCAGCAACCTGGGTACT
CCCAATCAGGGGGGTGGCTTCTTCCTGGAACCAGCACCCTGTGTCCACCTGCAAATCCTC
ATCCTCAGTTTGGAGGTGCCCTCTCCCTCCCCTCCACGCACAGCTGTGACAGGTACCCAA
CCCTGAGGAGCCACCGGTCCTCACCCTACCCCAGCCCCTATGCTCATCGGAACAATTCTC
CAACCTATTCTGACAACTCACCTGCATGTTTATCCATGCTGCAATCCCATGACAATTGGT
CCAGCCTTGGAATGCCTGCCCATCCCAGCATGCTCCCCGTGAGCCACAATGCCAGCCCAC
CTACCAGCTCCAGTCAGTACCCCAGCCTGTGGTCTGTGAGCAACGGCGCCGTCACCCCGG
GCTCCCAGGCAGCAGCCGTGTCCAACGGGCTGGGGGCCCAGTTCTTCCGGGGCTCCCCCG
CGCACTACACACCCCTCACCCATCCGGTCTCGGCGCCCTCTTCCTCGGGATCCCCACTGT
ACGAAGGGGCGGCCGCGGCCACAGACATCGTGGACAGCCAGTACGACGCCGCAGCCCAAG
GCCGCCTCATAGCCTCATGGACACCTGTGTCGCCACCTTCCATGTGAAGCAGCAAGGCCC
AGGTCCCGAAAGATGCAGTGACTTTTTGTCGTGGCAGCCAGTGGTGACTGGATTGACCTA
CTAGGTACCCAGTGGCAGTCTCAGGTTAAGAAGGAAATGCAGCCTCAGTAACTTCCTTTT
CAAAGCAGTGGAGGAGCACAC
>OriGene 5' read for NM_003181 unedited
GNNCGGCCGCGNAATTCGCCCTTGNAGCAGGNAGGTGGATTCTCAGTAGCGAGTCTGGGC
TTCGGGGACGGCGGGGAGGGGAGCCGGACGGGAGGATGAGCTCCCCTGGCACCGAGAGCG
CGGGAAAGAGCCTGCAGTACCGAGTGGACCACCTGCTGAGCGCCGTGGAGAATGAGCTGC
AGGCGGGCAGCGAGAAGGGCGACCCCACAGAGCGCGAACTGCGCGTGGGCCTGGAGGAGA
GCGAGCTGTGGCTGCGCTTCAAGGAGCTCACCAATGAGATGATCGTGACCAAGAACGGCA
GGAGGATGTTTCCGGTGCTGAAGGTGAACGTGTCTGGCCTGGACCCCAACGCCATGTACT
CCTTCCTGCTGGACTTCGTGGCGGCGGACAACCACCGCTGGAAGTACGTGAACGGNGAAT
GGGTGCCGGGGGGCAAGCCGGAGCCGCAGGCGCCCAGCTGCGTCTACATCCACCCCGACT
CGCCCAACTTCGGGGCCCACTGGATGAAGGCTCCCGTCTCCTTCAGCANAGTCAAGCTCA
CCAACNAGCTCAACGGGAGGNGGCCAGATCATGCTGAACTCCTTTGCATAGTATGAGCCT
CGAATCCACATAGTGAGAGTTGGGGGGTCCACAGCGCATGATCACCAGCCACTGCTTCCC
TGAGACCCAGTTCATAGCGGTGACTGCTTATCAGAACGAGGAGATCACAGCTCTTAAAAT
TAAGTANCATCCATTTGCAAAAGCTTTCCTTGATGCANAGGANAGAAGTGATCACAAAGA
GATGAATGGANGACCCGNAGACAGCCAGCAACCTGGGTACTCCCATCA
>Reverse primer walk for NM_003181 unedited
GACTTCTGGATAGTCAATCCATTCCCACTGGCTGCCACGAAAAAATCACTGCATCTTTCG
GGACCTGGGCCTTGCTGCTTCACATGGAAGGTGGCGACACAGGTGTCCATGAGGCTATGA
GGCGGCCTTGGGCTGCGGCGTCGTACTGGCTGTCCACGATGTCTGTGGCCGCGGCCGCCC
CTTCGTACAGTGGGGATCCCGAGGAAGAGGGCGCCGAGACCGGATGGGTGAGGGGTGTGT
AGTGCGCGGGGGAGCCCCGGAAGAACTGGGCCCCCAGCCCGTTGGACACGGCTGCTGCCT
GGGAGCCCGGGGTGACGGCGCCGTTGCTCACAGACCACAGGCTGGGGTACTGACTGGAGC
TGGTAGGTGGGCTGGCATTGTGGCTCACGGGGAGCATGCTGGGATGGGCAGGCATTCCAA
GGCTGGACCAATTGTCATGGGATTGCAGCATGGATAAACATGCAGGTGAGTTGTCAGAAT
AGGTTGGAGAATTGTTCCGATGAGCATAGGGGCTGGGGTAGGGTGAGGACCGGTGGCTCC
TCAGGGTTGGGTACCTGTCACAGCTGTGCGTGGAGGGGAGGGAGAGGGCACCTCCAAACT
GAGGATGAGGATTTGCAGGTGGACACAGGGTGCTGGTTCCAGGAAGAAGCCACCCCCCTG
ATTGGGAGTACCCAGGTTGCTGGCTGTCTCCGGGTTCCTCCATCATCTCTTTGTGATCAC
TTCTTTCCTTTGCATCAAGGAAAGCTTTTGCAAATGGATTGTACTTAATTTTAAGAGCTG
TGATCTCCTCGTTCTGATAAGCAGTCACCGCTATGAACTGGGTCTCAGGGAAGCAGTGGC
TA
Restriction Sites Please inquire     
ACCN NM_003181
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation There is 4 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the insertion of 1aa and substituion of 1 aa.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003181.2, NP_003172.1
RefSeq Size 2518 bp
RefSeq ORF 1308 bp
Locus ID 6862
Cytogenetics 6q27
Protein Families ES Cell Differentiation/IPS, Transcription Factors
Gene Summary 'The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. [provided by RefSeq, Sep 2018]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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