Cyclin E2 (CCNE2) (NM_057749) Human Tagged ORF Clone

CAT#: RC218597

CCNE2 (Myc-DDK-tagged)-Human cyclin E2 (CCNE2)

Product Images

Western blot validation of overexpression lysate (Cat# LY403299) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218597 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCNE2
• Synonyms: CYCE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218597 representing NM_057749
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAGACGAAGTAGCCGTTTACAAGCTAAGCAGCAGCCCCAGCCCAGCCAGACGGAATCCCCCCAAG
  AAGCCCAGATAATCCAGGCCAAGAAGAGGAAAACTACCCAGGATGTCAAAAAAAGAAGAGAGGAGGTCAC
  CAAGAAACATCAGTATGAAATTAGGAATTGTTGGCCACCTGTATTATCTGGGGGGATCAGTCCTTGCATT
  ATCATTGAAACACCTCACAAAGAAATAGGAACAAGTGATTTCTCCAGATTTACAAATTACAGATTTAAAA
  ATCTTTTTATTAATCCTTCACCTTTGCCTGATTTAAGCTGGGGATGTTCAAAAGAAGTCTGGCTAAACAT
  GTTAAAAAAGGAGAGCAGATATGTTCATGACAAACATTTTGAAGTTCTGCATTCTGACTTGGAACCACAG
  ATGAGGTCCATACTTCTAGACTGGCTTTTAGAGGTATGTGAAGTATACACACTTCATAGGGAAACATTTT
  ATCTTGCACAAGACTTTTTTGATAGATTTATGTTGACACAAAAGGATATAAATAAAAATATGCTTCAACT
  CATTGGAATTACCTCATTATTCATTGCTTCCAAACTTGAGGAAATCTATGCTCCTAAACTCCAAGAGTTT
  GCTTACGTCACTGATGGTGCTTGCAGTGAAGAGGATATCTTAAGGATGGAACTCATTATATTAAAGGCTT
  TAAAATGGGAACTTTGTCCTGTAACAATCATCTCCTGGCTAAATCTCTTTCTCCAAGTTGATGCTCTTAA
  AGATGCTCCTAAAGTTCTTCTACCTCAGTATTCTCAGGAAACATTCATTCAAATAGCTCAGCTTTTAGAT
  CTGTGTATTCTAGCCATTGATTCATTAGAGTTCCAGTACAGAATACTGACTGCTGCTGCCTTGTGCCATT
  TTACCTCCATTGAAGTGGTTAAGAAAGCCTCAGGTTTGGAGTGGGACAGTATTTCAGAATGTGTAGATTG
  GATGGTACCTTTTGTCAATGTAGTAAAAAGTACTAGTCCAGTGAAGCTGAAGACTTTTAAGAAGATTCCT
  ATGGAAGACAGACATAATATCCAGACACATACAAACTATTTGGCTATGCTGGAGGAAGTAAATTACATAA
  ACACCTTCAGAAAAGGGGGACAGTTGTCACCAGTGTGCAATGGAGGCATTATGACACCACCGAAGAGCAC
  TGAAAAACCACCAGGAAAACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218597 representing NM_057749
  Red=Cloning site Green=Tags(s)
  
  MSRRSSRLQAKQQPQPSQTESPQEAQIIQAKKRKTTQDVKKRREEVTKKHQYEIRNCWPPVLSGGISPCI
  IIETPHKEIGTSDFSRFTNYRFKNLFINPSPLPDLSWGCSKEVWLNMLKKESRYVHDKHFEVLHSDLEPQ
  MRSILLDWLLEVCEVYTLHRETFYLAQDFFDRFMLTQKDINKNMLQLIGITSLFIASKLEEIYAPKLQEF
  AYVTDGACSEEDILRMELIILKALKWELCPVTIISWLNLFLQVDALKDAPKVLLPQYSQETFIQIAQLLD
  LCILAIDSLEFQYRILTAAALCHFTSIEVVKKASGLEWDSISECVDWMVPFVNVVKSTSPVKLKTFKKIP
  MEDRHNIQTHTNYLAMLEEVNYINTFRKGGQLSPVCNGGIMTPPKSTEKPPGKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_057749
• ORF Size: 1212 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057749.1, NM_057749.2, NP_477097.1
• RefSeq Size: 2748 bp
• RefSeq ORF: 1215 bp
• Locus ID: 9134
• Domains: cyclin_C, CYCLIN, cyclin
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer
• MW: 46.6 kDa
• Gene Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq, Jul 2008]