KCNK7 (NM_033347) Human Tagged ORF Clone

CAT#: RC218633

KCNK7 (Myc-DDK-tagged)-Human potassium channel, subfamily K, member 7 (KCNK7), transcript variant A

Product Images

Western blot validation of overexpression lysate (Cat# LY409584) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218633 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNK7
• Synonyms: K2p7.1; TWIK3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218633 representing NM_033347
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGGTCTAAGGCCCTGGTCCCGATACGGGCTCCTGGTTGTGGCCCACTTGCTGGCCCTGGGGCTTG
  GGGCTGTGGTGTTCCAGGCCCTGGAGGGGCCTCCTGCATGCAGGCTTCAGGCTGAGCTCAGGGCAGAGCT
  GGCAGCCTTCCAGGCAGAGCATAGGGCCTGCCTGCCACCCGGAGCTCTGGAAGAGCTGCTGGGCACTGCC
  CTGGCCACCCAGGCCCATGGGGTCTCCACCCTGGGCAACAGCTCAGAGGGCAGGACCTGGGACCTTCCCT
  CAGCCCTGCTCTTCGCTGCCAGCATCCTCACCACCACAGGTTATGGCCACATGGCCCCACTATCGCCAGG
  CGGAAAGGCCTTCTGCATGGTCTATGCAGCCCTGGGGCTGCCAGCCTCCTTAGCTCTCGTGGCCACCCTG
  CGCCATTGCCTGCTGCCTGTGCTCAGCCGCCCACGTGCCTGGGTAGCGGTCCACTGGCAGCTGTCACCGG
  CCAGGGCTGCGCTGCTGCAGGCAGTTGCACTGGGACTGCTGGTGGCCAGCAGCTTTGTGCTGCTGCCAGC
  GCTGGTGCTGTGGGGCCTTCAGGGCGACTGCAGCCTGCTGGGGGCCGTCTACTTCTGCTTCAGCTCGCTC
  AGCACCATTGGCCTGGAGGACTTGCTGCCCGGCCGCGGCCGCAGCCTGCACCCCGTGATTTACCACCTGG
  GCCAGCTCGCACTTCTTGGTTACTTGCTTCTAGGACTCTTGGCCATGCTGCTGGCAGTGGAGACCTTCTC
  TGAGCTGCCGCAGGTCCGTGCCATGGGGAAGTTCTTCAGACCCAGTGGTCCTGTGACTGCTGAGGACCAA
  GGTGGCATCCTAGGGCAGGATGAACTGGCTCTGAGCACCCTGCCGCCCGCGGCCCCAGCTTCAGGACAAG
  CCCCTGCTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218633 representing NM_033347
  Red=Cloning site Green=Tags(s)
  
  MGGLRPWSRYGLLVVAHLLALGLGAVVFQALEGPPACRLQAELRAELAAFQAEHRACLPPGALEELLGTA
  LATQAHGVSTLGNSSEGRTWDLPSALLFAASILTTTGYGHMAPLSPGGKAFCMVYAALGLPASLALVATL
  RHCLLPVLSRPRAWVAVHWQLSPARAALLQAVALGLLVASSFVLLPALVLWGLQGDCSLLGAVYFCFSSL
  STIGLEDLLPGRGRSLHPVIYHLGQLALLGYLLLGLLAMLLAVETFSELPQVRAMGKFFRPSGPVTAEDQ
  GGILGQDELALSTLPPAAPASGQAPAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033347
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033347.1, NP_203133.1
• RefSeq Size: 1312 bp
• RefSeq ORF: 924 bp
• Locus ID: 10089
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 31.8 kDa
• Gene Summary: This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel; however, it may require other non-pore-forming proteins for activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]