TFIIE alpha (GTF2E1) (NM_005513) Human Tagged ORF Clone

CAT#: RC218763

GTF2E1 (Myc-DDK-tagged)-Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1)

Product Images

Western blot validation of overexpression lysate (Cat# LY417254) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218763 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GTF2E1 protein (Cat# TP318763). The protein was produced from HEK293T cells transfected with GTF2E1 cDNA clone (Cat# RC218763) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTF2E1
• Synonyms: FE; TF2E1; TFIIE-A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218763 representing NM_005513
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACCCAGATGTCCTCACTGAAGTTCCAGCAGCATTGAAGCGGTTAGCCAAGTATGTGATCCGGG
  GATTTTATGGCATTGAGCATGCCTTGGCCTTGGACATCTTGATCAGGAACTCCTGTGTGAAAGAGGAGGA
  TATGCTGGAGCTGCTCAAGTTTGATCGGAAGCAACTTCGATCAGTTTTGAATAATTTAAAGGGAGACAAG
  TTTATCAAATGCAGAATGAGGGTAGAGACTGCTGCAGACGGGAAAACCACTCGCCATAACTACTACTTCA
  TCAATTATCGTACTCTTGTTAATGTGGTAAAATATAAACTGGACCACATGAGAAGAAGAATTGAGACCGA
  TGAGAGAGATTCGACCAACCGGGCTTCCTTCAAATGTCCTGTCTGTAGTAGTACTTTCACAGACTTAGAA
  GCTAATCAGCTCTTTGATCCTATGACAGGAACTTTCCGCTGTACTTTTTGCCATACAGAGGTAGAAGAGG
  ATGAATCAGCAATGCCCAAAAAAGATGCACGCACACTTTTGGCAAGGTTTAATGAACAAATTGAGCCCAT
  TTATGCATTGCTTCGGGAGACAGAGGATGTGAACTTGGCCTATGAAATACTTGAGCCAGAACCCACAGAA
  ATCCCAGCCCTGAAACAGAGCAAGGACCATGCAGCAACTACTGCTGGAGCTGCTAGCCTAGCAGGTGGGC
  ACCACCGGGAAGCATGGGCCACCAAAGGTCCTTCCTATGAAGACTTATACACTCAGAATGTTGTCATTAA
  CATGGATGACCAAGAAGATCTTCATCGAGCCTCACTGGAAGGGAAATCTGCCAAAGAGAGGCCTATTTGG
  TTGAGAGAAAGCACTGTCCAAGGGGCATATGGTTCTGAAGATATGAAAGAAGGGGGCATAGATATGGACG
  CATTTCAGGAGCGTGAGGAAGGCCATGCTGGGCCTGATGACAACGAAGAGGTCATGCGAGCACTGCTCAT
  TCACGAGAAAAAGACTTCCTCTGCCATGGCTGGTTCAGTGGGGGCAGCTGCTCCAGTGACCGCTGCCAAT
  GGCGATGACTCAGAAAGCGAGACCAGTGAGTCAGATGATGATTCTCCACCCCGTCCGGCAGCTGTGGCTG
  TGCATAAACGAGAAGAGGATGAAGAGGAAGATGACGAGTTTGAAGAAGTAGCAGATGACCCCATTGTCAT
  GGTGGCTGGCCGTCCGTTCTCCTACAGTGAAGTGAGCCAACGGCCAGAGCTAGTGGCCCAGATGACACCA
  GAAGAAAAGGAAGCATATATAGCAATGGGACAACGCATGTTTGAGGACCTCTTTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218763 representing NM_005513
  Red=Cloning site Green=Tags(s)
  
  MADPDVLTEVPAALKRLAKYVIRGFYGIEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDK
  FIKCRMRVETAADGKTTRHNYYFINYRTLVNVVKYKLDHMRRRIETDERDSTNRASFKCPVCSSTFTDLE
  ANQLFDPMTGTFRCTFCHTEVEEDESAMPKKDARTLLARFNEQIEPIYALLRETEDVNLAYEILEPEPTE
  IPALKQSKDHAATTAGAASLAGGHHREAWATKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIW
  LRESTVQGAYGSEDMKEGGIDMDAFQEREEGHAGPDDNEEVMRALLIHEKKTSSAMAGSVGAAAPVTAAN
  GDDSESETSESDDDSPPRPAAVAVHKREEDEEEDDEFEEVADDPIVMVAGRPFSYSEVSQRPELVAQMTP
  EEKEAYIAMGQRMFEDLFE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005513
• ORF Size: 1317 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005513.1, NM_005513.2, NP_005504.1
• RefSeq Size: 2969 bp
• RefSeq ORF: 1320 bp
• Locus ID: 2960
• Cytogenetics: 3q13.33
• Domains: TFIIE
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 49.3 kDa
• Gene Summary: ''