TFIIE alpha (GTF2E1) (NM_005513) Human Untagged Clone

CAT#: SC116700

GTF2E1 (untagged)-Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GTF2E1
• Synonyms: FE; TF2E1; TFIIE-A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116700 sequence for NM_005513 edited (data generated by NextGen Sequencing)
  ATGGCAGACCCAGATGTCCTCACTGAAGTTCCAGCAGCATTGAAGCGGTTAGCCAAGTAT
  GTGATCCGGGGATTTTATGGCATTGAGCATGCCTTGGCCTTGGACATCTTGATCAGGAAC
  TCCTGTGTGAAAGAGGAGGATATGCTGGAGCTGCTCAAGTTTGATCGGAAGCAACTTCGA
  TCAGTTTTGAATAATTTAAAGGGAGACAAGTTTATCAAATGCAGAATGAGGGTAGAGACT
  GCTGCAGACGGGAAAACCACTCGCCATAACTACTACTTCATCAATTATCGTACTCTTGTT
  AATGTGGTAAAATATAAACTGGACCACATGAGAAGAAGAATTGAGACCGATGAGAGAGAT
  TCGACCAACCGGGCTTCCTTCAAATGTCCTGTCTGTAGTAGTACTTTCACAGACTTAGAA
  GCTAATCAGCTCTTTGATCCTATGACAGGAACTTTCCGCTGTACTTTTTGCCATACAGAG
  GTAGAAGAGGATGAATCAGCAATGCCCAAAAAAGATGCACGCACACTTTTGGCAAGGTTT
  AATGAACAAATTGAGCCCATTTATGCATTGCTTCGGGAGACAGAGGATGTGAACTTGGCC
  TATGAAATACTTGAGCCAGAACCCACAGAAATCCCAGCCCTGAAACAGAGCAAGGACCAT
  GCAGCAACTACTGCTGGAGCTGCTAGCCTAGCAGGTGGGCACCACCGGGAAGCATGGGCC
  ACCAAAGGTCCTTCCTATGAAGACTTATACACTCAGAATGTTGTCATTAACATGGATGAC
  CAAGAAGATCTTCATCGAGCCTCACTGGAAGGGAAATCTGCCAAAGAGAGGCCTATTTGG
  TTGAGAGAAAGCACTGTCCAAGGGGCATATGGTTCTGAAGATATGAAAGAAGGGGGCATA
  GATATGGACGCATTTCAGGAGCGTGAGGAAGGCCATGCTGGGCCTGATGACAACGAAGAG
  GTCATGCGAGCACTGCTCATTCACGAGAAAAAGACTTCCTCTGCCATGGCTGGTTCAGTG
  GGGGCAGCTGCTCCAGTGACCGCTGCCAATGGCAGTGACTCAGAAAGCGAGACCAGTGAG
  TCAGATGATGATTCTCCACCCCGTCCGGCAGCTGTGGCTGTGCATAAACGAGAAGAGGAT
  GAAGAGGAAGATGACGAGTTTGAAGAAGTAGCAGATGACCCCATTGTCATGGTGGCTGGC
  CGTCCGTTCTCCTACAGTGAAGTGAGCCAACGGCCAGAGCTAGTGGCCCAGATGACACCA
  GAAGAAAAGGAAGCATATATAGCAATGGGACAACGCATGTTTGAGGACCTCTTTGAGTGA
  
  Clone variation with respect to NM_005513.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005513 unedited
  GGGTCACATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCGCTGGGGG
  CAGCTGTAGTGGGAGTGTTCCAGGATTCGCCTTGTATATTTAAAGTTGGAGTTCGTTGCT
  AAAGATGGCAGACCCAGATGTCCTCACTGAAGTTCCAGCAGCATTGAAGCGGTTAGCCAA
  GTATGTGATCCGGGGATTTTATGGCATTGAGCATGCCTTGGCCTTGGACATCTTGATCAG
  GAACTCCTGTGTGAAAGAGGAGGATATGCTGGAGCTGCTCAAGTTTGATCGGAAGCAACT
  TCGATCAGTTTTGAATAATTTAAAGGGAGACAAGTTTATCAAATGCAGAATGAGGGTAGA
  GACTGCTGCAGACGGGAAAACCACTCGCCATAACTACTACTTCATCAATTATCGTACTCT
  TGTTAATGTGGTAAAATATAAACTGGACCACATGAGAAGAAGAATTGAGACCGATGAGAG
  AGATTCGACCAACCGGGCTTCCTTCAAATGTCCTGTCTGTAGTAGTACTTTCACAGACTT
  AGAAGCTAATCAGCTCTTTGATCCTATGACAGGAACTTTCCGCTGTACTTTTTGCCATAC
  AGAGGTAGAAGAGGATGAATCAGCAATGCCCAAAAAAGATGCACGCACACTTTTGGCAAG
  GTTTAATGAACAAATTGAGCCCATTTATGCATTGCTTCGGGAGACAGAGGATGTGAACTT
  GGCCTATGAAATACTTGAGCCAGAACCCACAGAAATCCCAGCCCTGAAACAGAGCAAGGA
  CCATGCAGNCACTACTGCTGGAGCTGCTAGCCTAGCAGGTGGGCACCACCGGGGAGCATG
  GGCCACCAAAGGTCCTTTCTATGAAGACTTATACACTCAGAATGTTGTCATTTACATGGA
  TGACCAAGAAGATCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005513 unedited
  GTATTTGTCACATTCAGTCTTAACACAACCCGGTAAATGAAACATCACTCCATACAAGGC
  TCATATATTCTTTACATGTGTACTATAAAATGATAAATACTTAAAAATTACAATGCAAGG
  AAAAATATTGTAAGATATACAATTGTTACAGACCCTTGTGTGAAAGTTCTTTATTTTTAA
  AGTTCTGCCTTAGGGAAAATACCATGCTACATATCAGAACCCAAAATAAACTGTTTCCTG
  AGGTTACAATGCTCAATGGTATACTGGATGCACCCTAAAATACTGTGTCTGACAAAAATT
  AAAAGAGAGACAGCCTTCAATGATGTTTAGAAAGAGGGAGAGTGACAGAGAGAGAGAAGA
  GTAATCAATTATTCTGTTGATTTCTGCTGGAATGGCCTCATTAAACAATTTATAGCACTG
  ACAAGAGGTCTGTACCCAAAGGGAACAAAACCAGGACCAGTCTCAGCTTTACATAAAGGA
  TTTCCAAATCAGACTAACTGACACAGTTCTAAGGCACTGTTACCAATGCTTGTCACTCTT
  CAACCCTTCACAGAGACTTCTTGTCTTACGTCCATGTTTTTCTAATTTCTTCCTAAGGGT
  TTACCAATTATACTTCTGTTTTCTATCATCCTAAATCCCAACCAAAAGACATTAANGCAT
  ACAATAAAATCTACCAGTCCTGGTCAGTTCTAAAGACACAAAATCTGGTTTAGACATTAT
  TCATAGACTCAGTTTATNTTTCCCCTTTCAACACGCTCCCATTNCCCTTCATATTGATCC
  ATCTAATTTTGAGGAATGCTCAATTCTAAGCAAAATTCAGGAATTTTTCCCCATAAGGAT
  TAGACTGTGGAAACTGTTTTGGTGGGCCCCATAAAAATTGNTGGAAGCCTGCTTGAAAAA
  TAAAGAGGGGACAGGATTTTGGACCTCTGGTTAACCAAGGTGGAAGGTTACCTTGAACTC
  CCTCCTTCTTTAAGGGAAAATTTTTTTTTAAGAGAACAAATAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005513
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005513.1, NP_005504.1
• RefSeq Size: 2969 bp
• RefSeq ORF: 1320 bp
• Locus ID: 2960
• Cytogenetics: 3q13.33
• Domains: TFIIE
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: ''