ABCG5 (NM_022436) Human Tagged ORF Clone

CAT#: RC218776

ABCG5 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family G (WHITE), member 5 (ABCG5)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCG5
• Synonyms: STSL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218776 representing NM_022436
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGACCTCTCATCTTTGACCCCCGGAGGGTCCATGGGTCTCCAAGTAAACAGAGGCTCCCAGAGCT
  CCCTGGAGGGGGCTCCTGCCACCGCCCCGGAGCCTCACAGCCTGGGCATCCTCCATGCCTCCTACAGCGT
  CAGCCACCGCGTGAGGCCCTGGTGGGACATCACATCTTGCCGGCAGCAGTGGACCAGGCAGATCCTCAAA
  GATGTCTCCTTGTACGTGGAGAGCGGGCAGATCATGTGCATCCTAGGAAGCTCAGGCTCCGGGAAAACCA
  CGCTGCTGGACGCCATGTCCGGGAGGCTGGGGCGCGCGGGGACCTTCCTGGGGGAGGTGTATGTGAACGG
  CCGGGCGCTGCGCCGGGAGCAGTTCCAGGACTGCTTCTCCTACGTCCTGCAGAGCGACACCCTGCTGAGC
  AGCCTCACCGTGCGCGAGACGCTGCACTACACCGCGCTGCTGGCCATCCGCCGCGGCAATCCCGGCTCCT
  TCCAGAAGAAGGTGGAGGCCGTCATGGCAGAGCTGAGTCTGAGCCATGTGGCAGACCGACTGATTGGCAA
  CTACAGCTTGGGGGGCATTTCCACGGGTGAGCGGCGCCGGGTCTCCATCGCAGCCCAGCTGCTCCAGGAT
  CCTAAGGTCATGCTGTTTGATGAGCCAACCACAGGCCTGGACTGCATGACTGCTAATCAGATTGTCGTCC
  TCCTGGTGGAACTGGCTCGCAGGAACCGAATTGTGGTTCTCACCATTCACCAGCCCCGTTCTGAGCTTTT
  TCAGCTCTTTGACAAAATTGCCATCCTGAGCTTCGGAGAGCTGATTTTCTGTGGCACGCCAGCGGAAATG
  CTTGATTTCTTCAATGACTGCGGTTACCCTTGTCCTGAACATTCAAACCCTTTTGACTTCTATATGGACC
  TGACGTCAGTGGATACCCAAAGCAAGGAACGGGAAATAGAAACCTCCAAGAGAGTCCAGATGATAGAATC
  TGCCTACAAGAAATCAGCAATTTGTCATAAAACTTTGAAGAATATTGAAAGAATGAAACACCTGAAAACG
  TTACCAATGGTTCCTTTCAAAACCAAAGATTCTCCTGGAGTTTTCTCTAAACTGGGTGTTCTCCTGAGGA
  GAGTGACAAGAAACTTGGTGAGAAATAAGCTGGCAGTGATTACGCGTCTCCTTCAGAATCTGATCATGGG
  TTTGTTCCTCCTTTTCTTCGTTCTGCGGGTCCGAAGCAATGTGCTAAAGGGTGCTATCCAGGACCGCGTA
  GGTCTCCTTTACCAGTTTGTGGGCGCCACCCCGTACACAGGCATGCTGAACGCTGTGAATCTGTTTCCCG
  TGCTGCGAGCTGTCAGCGACCAGGAGAGTCAGGACGGCCTCTACCAGAAGTGGCAGATGATGCTGGCCTA
  TGCACTGCACGTCCTCCCCTTCAGCGTTGTTGCCACCATGATTTTCAGCAGTGTGTGCTACTGGACGCTG
  GGCTTACATCCTGAGGTTGCCCGATTTGGATATTTTTCTGCTGCTCTCTTGGCCCCCCACTTAATTGGTG
  AATTTCTAACTCTTGTGCTACTTGGTATCGTCCAAAATCCAAATATAGTCAACAGTGTAGTGGCTCTGCT
  GTCCATTGCGGGGGTGCTTGTTGGATCTGGATTCCTCAGAAACATACAAGAAATGCCCATTCCTTTTAAA
  ATCATCAGTTATTTTACATTCCAAAAATATTGCAGTGAGATTCTTGTAGTCAATGAGTTCTACGGACTGA
  ATTTCACTTGTGGCAGCTCAAATGTTTCTGTGACAACTAATCCAATGTGTGCCTTCACTCAAGGAATTCA
  ATTCATTGAGAAAACCTGCCCAGGTGCAACATCTAGATTCACAATGAACTTTCTGATTTTGTATTCATTT
  ATTCCAGCTCTTGTCATCCTAGGAATAGTTGTTTTCAAAATAAGGGATCATCTCATTAGCAGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218776 representing NM_022436
  Red=Cloning site Green=Tags(s)
  
  MGDLSSLTPGGSMGLQVNRGSQSSLEGAPATAPEPHSLGILHASYSVSHRVRPWWDITSCRQQWTRQILK
  DVSLYVESGQIMCILGSSGSGKTTLLDAMSGRLGRAGTFLGEVYVNGRALRREQFQDCFSYVLQSDTLLS
  SLTVRETLHYTALLAIRRGNPGSFQKKVEAVMAELSLSHVADRLIGNYSLGGISTGERRRVSIAAQLLQD
  PKVMLFDEPTTGLDCMTANQIVVLLVELARRNRIVVLTIHQPRSELFQLFDKIAILSFGELIFCGTPAEM
  LDFFNDCGYPCPEHSNPFDFYMDLTSVDTQSKEREIETSKRVQMIESAYKKSAICHKTLKNIERMKHLKT
  LPMVPFKTKDSPGVFSKLGVLLRRVTRNLVRNKLAVITRLLQNLIMGLFLLFFVLRVRSNVLKGAIQDRV
  GLLYQFVGATPYTGMLNAVNLFPVLRAVSDQESQDGLYQKWQMMLAYALHVLPFSVVATMIFSSVCYWTL
  GLHPEVARFGYFSAALLAPHLIGEFLTLVLLGIVQNPNIVNSVVALLSIAGVLVGSGFLRNIQEMPIPFK
  IISYFTFQKYCSEILVVNEFYGLNFTCGSSNVSVTTNPMCAFTQGIQFIEKTCPGATSRFTMNFLILYSF
  IPALVILGIVVFKIRDHLISR
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022436
• ORF Size: 1953 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_022436.1, NM_022436.2, NP_071881.1
• RefSeq Size: 2740
• RefSeq ORF: 1956
• Locus ID: 64240
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 72.5 kDa
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008]