Dynamin 1 (DNM1) (NM_004408) Human Tagged ORF Clone

CAT#: RC218819

DNM1 (Myc-DDK-tagged)-Human dynamin 1 (DNM1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418003) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218819 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNM1
• Synonyms: DNM; EIEE31
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218819 representing NM_004408
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACCGCGGCATGGAAGATCTCATCCCGCTGGTCAACCGGCTGCAAGACGCCTTCTCTGCCATCG
  GCCAGAACGCGGACCTCGACCTGCCGCAGATCGCTGTGGTGGGCGGCCAGAGCGCCGGCAAGAGCTCGGT
  GCTCGAGAATTTCGTAGGCAGGGACTTCTTGCCTCGAGGATCTGGCATTGTCACCCGACGTCCCCTGGTC
  TTGCAGCTGGTCAATGCAACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGAAAGAAATTCACCGACT
  TCGAGGAGGTGCGCCTTGAGATCGAGGCCGAGACCGACAGGGTCACCGGCACCAACAAGGGCATCTCGCC
  GGTGCCTATCAACCTCCGCGTCTACTCGCCGCACGTGCTGAACCTGACCCTGGTGGACCTGCCCGGAATG
  ACCAAGGTCCCGGTGGGGGACCAACCTCCCGACATCGAGTTCCAGATCCGAGACATGCTTATGCAGTTTG
  TCACCAAGGAGAACTGCCTCATCCTGGCCGTGTCCCCCGCCAACTCTGACCTGGCCAATTCTGACGCCCT
  CAAGGTCGCCAAGGAGGTGGACCCCCAGGGCCAGCGCACCATCGGGGTCATCACCAAGCTGGACCTGATG
  GACGAGGGCACAGATGCCCGTGATGTGCTGGAGAACAAGCTGCTCCCCCTGCGCAGAGGCTACATTGGAG
  TGGTGAACCGGAGCCAGAAGGACATTGATGGCAAGAAGGACATTACCGCCGCCTTGGCTGCTGAACGAAA
  GTTCTTCCTCTCCCATCCATCTTATCGCCACTTGGCTGACCGTATGGGCACGCCCTACCTGCAGAAGGTC
  CTCAATCAGCAACTGACGAACCACATCCGGGACACACTGCCGGGGCTGCGGAACAAGCTGCAGAGCCAGC
  TACTGTCCATTGAGAAGGAGGTGGAGGAATACAAGAACTTCCGCCCTGATGACCCAGCTCGCAAGACCAA
  GGCCCTGCTGCAGATGGTCCAGCAGTTCGCCGTAGACTTTGAGAAGCGCATTGAGGGCTCAGGAGATCAG
  ATCGACACCTACGAACTGTCAGGGGGAGCCCGCATTAACCGAATCTTCCACGAGCGCTTCCCTTTCGAGC
  TGGTCAAGATGGAGTTTGATGAGAAGGAACTCCGAAGGGAGATCAGCTATGCTATCAAGAATATCCATGG
  CATTAGAACGGGGCTGTTTACCCCAGACATGGCCTTTGAGACCATTGTGAAAAAGCAGGTGAAGAAGATC
  CGAGAACCGTGTCTCAAGTGTGTGGACATGGTTATCTCGGAGCTAATCAGCACCGTTAGACAGTGCACCA
  AGAAGCTCCAGCAGTACCCGCGGCTACGGGAGGAGATGGAGCGCATCGTGACCACCCACATCCGGGAGCG
  CGAGGGCCGCACTAAGGAGCAGGTCATGCTTCTCATCGATATCGAGCTGGCTTACATGAACACCAACCAT
  GAGGACTTCATAGGCTTTGCCAATGCTCAGCAGAGGAGCAACCAGATGAACAAGAAGAAGACTTCAGGGA
  ACCAGGATGAGATTCTGGTCATCCGCAAGGGCTGGCTGACTATCAATAATATTGGCATCATGAAAGGGGG
  CTCCAAGGAGTACTGGTTTGTGCTGACTGCTGAGAATCTGTCCTGGTACAAGGATGATGAGGAGAAAGAG
  AAGAAATACATGCTGTCTGTGGACAACCTCAAGCTGCGGGACGTGGAGAAGGGCTTTATGTCGAGCAAGC
  ATATCTTTGCCCTCTTTAACACGGAGCAGAGGAATGTCTACAAGGATTATCGGCAGCTGGAGCTAGCCTG
  TGAGACACAGGAGGAGGTGGACAGCTGGAAGGCCTCCTTCCTGAGGGCTGGCGTGTACCCTGAGCGTGTT
  GGGGACAAAGAGAAAGCCAGCGAGACCGAGGAGAATGGCTCCGACAGCTTCATGCATTCCATGGACCCAC
  AGCTGGAACGGCAAGTGGAGACCATCCGGAATCTTGTGGACTCATACATGGCCATTGTCAACAAGACCGT
  GAGGGACCTCATGCCCAAGACCATCATGCACCTCATGATTAACAATACCAAGGAGTTCATCTTCTCGGAG
  CTGCTGGCCAACCTGTACTCGTGTGGGGACCAGAACACGCTGATGGAGGAGTCGGCGGAGCAGGCACAGC
  GGCGCGACGAGATGCTGCGCATGTACCACGCACTGAAGGAGGCGCTCAGCATCATCGGCGACATCAACAC
  GACCACCGTCAGCACGCCCATGCCCCCGCCCGTGGACGACTCCTGGCTGCAGGTGCAGAGCGTACCGGCC
  GGACGCAGGTCGCCCACGTCCAGCCCCACGCCGCAGCGCCGAGCCCCCGCCGTGCCCCCAGCCCGGCCCG
  GGTCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCTGGGTCCGCCCTGGGGGGGGCGCCCCCCGTGCCCTC
  CAGGCCGGGGGCTTCCCCTGACCCTTTCGGCCCTCCCCCTCAGGTGCCCTCGCGCCCCAACCGCGCCCCG
  CCCGGGGTCCCCAGCCGATCGGGTCAGGCAAGTCCATCCCGTCCTGAGAGCCCCAGGCCCCCCTTCGACC
  TC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218819 representing NM_004408
  Red=Cloning site Green=Tags(s)
  
  MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLV
  LQLVNATTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLPGM
  TKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALKVAKEVDPQGQRTIGVITKLDLM
  DEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPYLQKV
  LNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVEEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIEGSGDQ
  IDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDMAFETIVKKQVKKI
  REPCLKCVDMVISELISTVRQCTKKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIELAYMNTNH
  EDFIGFANAQQRSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKE
  KKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERV
  GDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSE
  LLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPA
  GRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGPPPQVPSRPNRAP
  PGVPSRSGQASPSRPESPRPPFDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004408
• ORF Size: 2592 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004408.1, NM_004408.2, NM_004408.3, NP_004399.2
• RefSeq Size: 3216 bp
• RefSeq ORF: 2595 bp
• Locus ID: 1759
• Cytogenetics: 9q34.11
• Domains: dynamin_2, dynamin, PH, GED
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• MW: 97.2 kDa
• Gene Summary: 'This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'