Dynamin 1 (DNM1) (NM_004408) Human Untagged Clone

CAT#: SC310077

DNM1 (untagged)-Human dynamin 1 (DNM1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNM1
• Synonyms: DNM; EIEE31
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004408 edited
  AGTCGGGGCGCGCGGCTGCAGCGGCGGAGCCGGAGTCGGAGCCGGGAGCGCTAGCGGCAG
  CCGGATCGCAGCCTGCGGGGCCAGCCGCAGCCATGGGCAACCGCGGCATGGAAGATCTCA
  TCCCGCTGGTCAACCGGCTGCAAGACGCCTTCTCTGCCATCGGCCAGAACGCGGACCTCG
  ACCTGCCGCAGATCGCTGTGGTGGGCGGCCAGAGCGCCGGCAAGAGCTCGGTGCTCGAGA
  ATTTCGTAGGCAGGGACTTCTTGCCTCGAGGATCTGGCATTGTCACCCGACGTCCCCTGG
  TCTTGCAGCTGGTCAATGCAACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGAAAGA
  AATTCACCGACTTCGAGGAGGTGCGCCTTGAGATCGAGGCCGAGACCGACAGGGTCACCG
  GCACCAACAAGGGCATCTCGCCGGTGCCTATCAACCTCCGCGTCTACTCGCCGCACGTGC
  TGAACCTGACCCTGGTGGACCTGCCCGGAATGACCAAGGTCCCGGTGGGGGACCAACCTC
  CCGACATCGAGTTCCAGATCCGAGACATGCTTATGCAGTTTGTCACCAAGGAGAACTGCC
  TCATCCTGGCCGTGTCCCCCGCCAACTCTGACCTGGCCAATTCTGACGCCCTCGAGGTCG
  CCAAGGAGGTGGACCCCCAGGGCCAGCGCACCATCGGGGTCATCACCAAGCTGGACCTGA
  TGGACGAGGGCACAGATGCCCGTGATGTGCTGGAGAACAAGCTGCTCCCCCTGCGCAGAG
  GCTACATTGGAGTGGTGAACCGGAGCCAGAAGGACATTGATGGCAAGAAGGACATTACCG
  CCGCCTTGGCTGCTGAACGAAAGTTCTTCCTCTCCCATCCATCTTATCGCCACTTGGCTG
  ACCGTATGGGCACGCCCTACCTGCAGAAGGTCCTCAATCAGCAACTGACGGACCACATCC
  GGGACACACTGCCGGGGCTGCGGAACAAGCTGCAGAGCCAGCTACTGTCCATTGAGAAGG
  AGGTGGAGGAATACAAGAACTTCCGCCCTGATGACCCAGCTCGCAAGACCAAGGCCCTGC
  TGCAGATGGTCCAGCAGTTCGCCGTAGACTTTGAGAAGCGCATTGAGGGCTCAGGAGATC
  AGATCGACACCTACGAACTGTCAGGGGGAGCCCGCATTAACCGAATCTTCCACGAGCGCT
  TCCCTTTCGAGCTGGTCAAGATGGAGTTTGATGAGAAGGAACTCCGAAGGGAGATCAGCT
  ATGCTATCAAGAATATCCATGGCATTAGAACGGGGCTGTTTACCCCAGACATGGCCTTTG
  AGACCATTGTGAAAAAGCAGGTGAAGAAGATCCGAGAACCGTGTCTCAAGTGTGTGGACA
  TGGTTATCTCGGAGCTAATCAGCACCGTTAGACAGTGCACCAAGAAGCTCCAGCAGTACC
  CGCGGCTACGGGAGGAGATGGAGCGCATCGTGACCACCCACATCCGGGAGCGCGAGGGCC
  GCACTAAGGAGCAGGTCATGCTTCTCATCGATATCGAGCTGGCTTACATGAACACCAACC
  ATGAGGACTTCATAGGCTTTGCCAATGCTCAGCAGAGGAGCAACCAGATGAACAAGAAGA
  AGACTTCAGGGAACCAGGATGAGATTCTGGTCATCCGCAAGGGCTGGCTGACTATCAATA
  ATATTGGCATCATGAAAGGGGGCTCCAAGGAGTACTGGTTTGTGCTGACTGCTGAGAATC
  TGTCCTGGTACAAGGATGATGAGGAGAAAGAGAAGAAATACATGCTGTCTGTGGACAACC
  TCAAGCTGCGGGACGTGGAGAAGGGCTTTATGTCGAGCAAGCATATCTTTGCCCTCTTTA
  ACACGGAGCAGAGGAATGTCTACAAGGATTATCGGCAGCTGGAGCTAGCCTGTGAGACAC
  AGGAGGAGGTGGACAGCTGGAAGGCCTCCTTCCTGAGGGCTGGCGTGTACCCTGAGCGTG
  TTGGGGACAAAGAGAAAGCCAGCGAGACCGAGGAGAATGGCTCCGACAGCTTCATGCATT
  CCATGGACCCACAGCTGGAACGGCAAGTGGAGACCATCCGGAATCTTGTGGACTCATACA
  TGGCCATTGTCAACAAGACCGTGAGGGACCTCATGCCCAAGACCATCATGCACCTCATGA
  TTAACAATACCAAGGAGTTCATCTTCTCGGAGCTGCTGGCCAACCTGTACTCGTGTGGGG
  ACCAGAACACGCTGATGGAGGAGTCGGCGGAGCAGGCACAGCGGCGCGACGAGATGCTGC
  GCATGTACCACGCACTGAAGGAGGCGCTCAGCATCATCGGCGACATCAACACGACCACCG
  TCAGCACGCCCATGCCCCCGCCCGTGGACGACTCCTGGCTGCAGGTGCAGAGCGTACCGG
  CCGGACGCAGGTCGCCCACGTCCAGCCCCACGCCGCAGCGCCGAGCCCCCGCCGTGCCCC
  CAGCCCGGCCCGGGTCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCTGGGTCCGCCATGG
  GGGGGGCGCCCCCCGTGCCCTCCAGGCCGGGGGCTTCCCCTGACCCTTTCGGCCCTCCCC
  CTCAGGTGCCCTCGCGCCCCAACCGCGCCCCGCCCGGGGTCCCCAGCCGATCGGGTCAGG
  CAAGTCCATCCCGTCCTGAGAGCCCCAGGCCCCCCTTCGACCTCTAAACAGATCCCTCCT
  CTTCTCGGAGACCTCCCTTTCCAAGCCTGCCTGGACGGCTGTTCTGTGACTTGACAGTGG
  CTCCCCCAGCCCCAAAGCCAGCCCCCTTCATCTGTGACTTAATCTGTTGTAGTGGTGAGC
  TGATACATTCAGGTGTGACCGTTGGTGAAAACTTGTGCCCCTTCTGTGGTATGCCCTTGC
  CCTGTTCTATAAATATCTATAAATACTCATATATATACACACCTACACATGGCCAACCGC
  CTCGCCTCTAGCGCTGGGAATCAGTCACTGTGCTATCCTTGTGGAGTCTTGTGGCCCAAC
  TACCAGAGAACGCTGTCCCCCGACATCCCACTCCAAAGTGTGCCACCTCCAGTGAGCCTC
  CTTGTCATGCCCGGCCTGTGGACAGCCAGCCCCCGCCATCCCTCCCACCCCCTACCAAGC
  ATGGGGGTGCTGTGCAGGCAGCCGTGTGGCCTGACAGTTTCTACCAGTCCTGCTGTCCCT
  CGGCTGAGAATAAAACCCATTTCTGGATGAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_004408
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain 3 SNPs compared with NM_004408.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004408.2, NP_004399.2
• RefSeq Size: 3216 bp
• RefSeq ORF: 2595 bp
• Locus ID: 1759
• Cytogenetics: 9q34.11
• Domains: dynamin_2, dynamin, PH, GED
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: 'This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Isoform 1 has been referred to as internal form 1 and the long C-terminal form.