Collagen XI alpha 2 (COL11A2) (NM_080681) Human Tagged ORF Clone

CAT#: RC218868

COL11A2 (Myc-DDK-tagged)-Human collagen, type XI, alpha 2 (COL11A2), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: COL11A2
• Synonyms: DFNA13; DFNB53; FBCG2; HKE5; OSMEDA; OSMEDB; PARP; STL3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218868 representing NM_080681
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGTGCAGCCGCTGCCATCGCCTCCTCCTCCTCCTACCTCTGGTGCTGGGGCTGAGCGCGGCCC
  CAGGCTGGGCAGGTGCACCCCCTGTGGATGTGCTCCGGGCCCTGAGGTTCCCCTCCCTCCCTGATGGTGT
  CCGGAGAGCGAAAGGCATCTGTCCAGCTGATGTGGCCTACCGAGTGGCACGACCTGCCCAGCTCAGTGCA
  CCCACTCGCCAGCTTTTCCCAGGAGGATTTCCCAAAGATTTCTCTCTGCTGACTGTTGTCCGGACCCGCC
  CTGGTCTCCAAGCTCCCCTCCTGACTCTCTACAGTGCCCAGGGTGTCCGACAGCTGGGCCTGGAGCTGGG
  CCGACCTGTCCGCTTCCTGTATGAAGACCAGACTGGGCGGCCTCAACCTCCCTCTCAGCCAGTCTTCCGA
  GGCCTCAGCCTAGCAGATGGCAAGTGGCACCGTGTGGCTGTGGCTGTGAAGGGCCAGTCTGTCACCCTCA
  TTGTTGACTGCAAGAAGCGAGTCACCCGGCCTCTCCCCCGAAGTGCTCGTCCAGTATTGGACACCCATGG
  AGTGATCATCTTTGGTGCCCGTATTCTGGATGAAGAAGTCTTTGAGGGTGATGTCCAGGAGCTGGCCATT
  GTCCCAGGGGTCCAGGCAGCCTATGAATCATGTGAACAGAAGGAGCTGGAATGCGAGGGGGGCCAGAGGG
  AAAGACCCCAAAACCAACAGCCTCACAGAGCCCAGAGATCTCCACAGCAGCAACCATCAAGACTTCACAG
  GCCACAAAATCAGGAACCCCAGAGCCAGGACCCCACCCCAGGTGAAGAGGAAGAAATCCTGGAGTCGAGC
  CTCTTGCCACCCCTTGAGGAGGCTGCCCATGGACCCCGAGGGCTGAAGGGAGAGAAAGGAGAGCCTGCAG
  TGTTGGAACCTGGTATGCTCGTGGAGGGGCCCCCTGGCCCAGAAGGCCCTGCGGGATTGATTGGTCCCCC
  TGGCATCCAGGGGAACCCAGGCCCAGTTGGAGACCCTGGAGAGAGGGGCCCCCCTGGCCGAGCAGGGCTC
  CCTGGATCAGATGGGGCTCCTGGTCCTCCTGGCACATCTCTCATGCTCCCATTCCGGTTTGGCAGTGGTG
  GGGGTGACAAGGGCCCTGTGGTGGCGGCCCAGGAGGCTCAGGCCCAGGCGATCCTGCAGCAGGCGAGGCT
  GGCGCTCCGTGGACCCCCTGGCCCCATGGGATACACAGGGCGCCCTGGACCCTTGGGCCAACCTGGGAGC
  CCTGGCCTGAAAGGAGAGTCTGGAGACTTAGGACCTCAGGGCCCCAGAGGACCTCAGGGCCTCACAGGCC
  CTCCTGGCAAGGCTGGGCGAAGGGGCCGGGCAGGTGCTGATGGAGCCCGAGGGATGCCTGGAGATCCTGG
  AGTGAAGGGTGACCGAGGTTTTGATGGACTCCCAGGGCTCCCTGGAGAGAAGGGCCATAGGGGTGATACT
  GGTGCCCAGGGCCTTCCTGGTCCCCCTGGTGAGGATGGAGAGAGGGGAGATGACGGGGAGATTGGGCCTC
  GAGGGCTGCCTGGAGAGTCGGGACCTCGAGGTCTCCTTGGCCCCAAAGGCCCACCTGGTATTCCTGGACC
  CCCTGGCGTCCGAGGCATGGATGGTCCCCAGGGCCCCAAAGGGAGCTTGGGACCCCAGGGAGAGCCAGGA
  CCTCCTGGACAACAGGGCACCCCTGGGACCCAGGGTCTTCCCGGGCCCCAGGGTGCCATCGGCCCTCATG
  GAGAGAAGGGTCCTCAAGGGAAGCCAGGGCTCCCCGGCATGCCTGGCTCAGACGGACCCCCGGGTCACCC
  AGGGAAGGAAGGTCCCCCTGGAACCAAAGGAAACCAGGGTCCCTCTGGACCTCAGGGACCTCTAGGATAC
  CCAGGACCTCGAGGGGTCAAGGGTGTGGACGGAATTCGGGGTCTGAAGGGTCATAAGGGTGAGAAGGGTG
  AGGATGGCTTTCCTGGGTTCAAAGGTGACATAGGCGTGAAAGGTGACAGGGGCGAAGTTGGAGTCCCTGG
  TTCCAGGGGAGAGGATGGTCCTGAGGGGCCAAAGGGACGCACTGGACCGACTGGAGACCCTGGGCCCCCA
  GGGCTCATGGGCGAGAAGGGCAAGCTGGGTGTTCCTGGTCTGCCTGGCTATCCTGGACGTCAGGGACCCA
  AGGGGTCCCTAGGATTTCCTGGCTTTCCTGGTGCCAGTGGAGAGAAGGGAGCCCGGGGCCTGTCGGGGAA
  GTCAGGGCCTCGGGGAGAACGGGGCCCCACGGGTCCACGGGGTCAGCGGGGACCCCGAGGTGCCACTGGG
  AAGTCTGGAGCTAAGGGAACATCTGGTGGTGATGGCCCCCATGGGCCCCCTGGAGAGAGGGGCCTCCCTG
  GACCTCAGGGTCCCAACGGGTTTCCTGGACCGAAAGGACCCCCGGGCCCCCCTGGGAAGGATGGGCTGCC
  GGGACACCCAGGCCAAAGAGGAGAAGTGGGTTTCCAAGGGAAGACCGGCCCCCCTGGTCCTCCAGGAGTG
  GTGGGACCTCAGGGAGCAGCAGGAGAAACCGGCCCTATGGGGGAGAGAGGTCACCCAGGCCCCCCGGGGC
  CCCCTGGAGAGCAGGGACTACCTGGGACAGCTGGAAAAGAAGGAACAAAGGGTGACCCTGGTCCCCCTGG
  GGCCCCAGGGAAGGATGGTCCTGCTGGTCTGAGGGGATTCCCAGGAGAGAGAGGCCTCCCAGGCACTGCT
  GGTGGACCTGGTTTGAAGGGGAATGAAGGTCCGTCTGGCCCCCCTGGCCCTGCAGGCTCCCCTGGGGAAC
  GAGGTGCAGCAGGATCAGGGGGACCCATTGGTCCGCCAGGGCGCCCAGGCCCGCAGGGTCCCCCTGGAGC
  AGCAGGAGAGAAAGGTGTCCCAGGTGAGAAGGGCCCCATTGGCCCGACTGGCCGAGATGGAGTGCAGGGT
  CCTGTGGGGCTTCCTGGTCCTGCTGGGCCTCCAGGTGTGGCTGGAGAGGATGGAGACAAGGGTGAGGTGG
  GGGACCCCGGACAGAAGGGCACCAAAGGGAACAAGGGTGAACATGGCCCTCCTGGACCCCCTGGACCCAT
  TGGTCCTGTGGGGCAGCCTGGAGCAGCGGGAGCAGATGGGGAGCCCGGAGCTCGGGGACCCCAGGGACAC
  TTTGGAGCCAAAGGTGATGAAGGAACAAGAGGATTCAATGGGCCCCCAGGACCCATTGGCCTACAGGGTT
  TGCCAGGCCCCTCTGGGGAGAAGGGAGAAACAGGAGATGTGGGTCCTATGGGACCACCTGGCCCCCCAGG
  ACCTCGAGGTCCAGCTGGACCCAATGGCGCTGATGGCCCACAAGGTCCCCCAGGAGGTGTTGGGAACCTG
  GGTCCCCCTGGAGAGAAGGGGGAACCAGGAGAGTCAGGATCTCCAGGGATCCAGGGCGAGCCAGGTGTCA
  AGGGTCCACGCGGGGAACGTGGAGAGAAAGGAGAGTCGGGGCAGCCAGGAGAGCCAGGGCCACCAGGGCC
  TAAAGGCCCCACAGGCGATGATGGCCCCAAAGGGAACCCTGGTCCTGTTGGTTTTCCTGGTGACCCTGGC
  CCCCCTGGAGAAGGTGGCCCTCGGGGCCAGGATGGTGCTAAGGGTGACCGAGGCGAGGATGGTGAGCCAG
  GACAGCCTGGATCCCCTGGTCCCACCGGGGAGAATGGACCCCCAGGGCCACTTGGAAAGCGAGGTCCTGC
  TGGCTCGCCTGGTTCCGAGGGGCGACAAGGAGGGAAGGGAGCCAAGGGAGATCCTGGCGCTATAGGTGCC
  CCGGGGAAGACAGGCCCGGTGGGTCCTGCAGGCCCAGCAGGGAAACCTGGCCCTGATGGTCTGAGGGGGC
  TCCCAGGCTCAGTGGGTCAGCAAGGCCGACCTGGAGCTACAGGCCAGGCTGGGCCCCCAGGTCCTGTGGG
  ACCCCCAGGGCTGCCTGGTCTCCGGGGCGATGCTGGAGCCAAGGGAGAGAAGGGCCACCCAGGTCTCATT
  GGACTGATTGGGCCCCCGGGTGAGCAGGGAGAGAAGGGAGATCGGGGACTTCCTGGGCCTCAGGGCTCCC
  CTGGGCAGAAGGGTGAGATGGGTATCCCAGGAGCATCCGGCCCCATTGGTCCTGGAGGTCCCCCCGGCCT
  CCCCGGACCTGCTGGCCCCAAAGGAGCCAAAGGAGCCACAGGCCCAGGCGGACCCAAGGGAGAGAAGGGT
  GTGCAGGGCCCTCCAGGACACCCGGGTCCCCCAGGCGAGGTGATCCAGCCACTGCCCATTCAGATGCCCA
  AGAAGACTCGGCGCTCGGTGGATGGAAGCCGTCTGATGCAGGAAGATGAGGCCATACCGACCGGGGGAGC
  CCCCGGCAGTCCTGGGGGGCTGGAGGAGATCTTTGGCTCACTCGACTCCCTGCGGGAGGAGATCGAGCAG
  ATGAGGCGGCCAACAGGGACCCAGGACAGCCCTGCTCGCACCTGCCAGGACCTGAAGCTGTGCCACCCAG
  AGCTTCCCGATGGAGAGTACTGGGTCGACCCCAACCAGGGCTGTGCTCGGGATGCCTTCCGAGTTTTCTG
  CAACTTCACAGCAGGGGGTGAGACCTGTGTGACGCCTAGGGATGACGTCACGCAGTTCTCTTACGTGGAC
  TCAGAGGGCTCCCCAGTGGGTGTGGTCCAGCTCACCTTCCTGCGGCTGCTCAGCGTCTCAGCCCACCAGG
  ACGTCTCCTACCCCTGCTCTGGAGCAGCCCGTGACGGTCCCCTGAGACTCCGTGGGGCCAATGAGGATGA
  GCTGAGCCCGGAGACTAGCCCCTATGTCAAAGAATTCAGAGATGGCTGCCAGACACAGCAAGGCCGGACG
  GTGCTGGAGGTGCGAACGCCTGTGCTGGAGCAGCTGCCAGTGCTGGATGCCTCCTTCTCAGACCTGGGAG
  CCCCACCGAGGCGGGGAGGGGTGCTGCTGGGGCCTGTCTGCTTCATGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218868 representing NM_080681
  Red=Cloning site Green=Tags(s)
  
  MERCSRCHRLLLLLPLVLGLSAAPGWAGAPPVDVLRALRFPSLPDGVRRAKGICPADVAYRVARPAQLSA
  PTRQLFPGGFPKDFSLLTVVRTRPGLQAPLLTLYSAQGVRQLGLELGRPVRFLYEDQTGRPQPPSQPVFR
  GLSLADGKWHRVAVAVKGQSVTLIVDCKKRVTRPLPRSARPVLDTHGVIIFGARILDEEVFEGDVQELAI
  VPGVQAAYESCEQKELECEGGQRERPQNQQPHRAQRSPQQQPSRLHRPQNQEPQSQDPTPGEEEEILESS
  LLPPLEEAAHGPRGLKGEKGEPAVLEPGMLVEGPPGPEGPAGLIGPPGIQGNPGPVGDPGERGPPGRAGL
  PGSDGAPGPPGTSLMLPFRFGSGGGDKGPVVAAQEAQAQAILQQARLALRGPPGPMGYTGRPGPLGQPGS
  PGLKGESGDLGPQGPRGPQGLTGPPGKAGRRGRAGADGARGMPGDPGVKGDRGFDGLPGLPGEKGHRGDT
  GAQGLPGPPGEDGERGDDGEIGPRGLPGESGPRGLLGPKGPPGIPGPPGVRGMDGPQGPKGSLGPQGEPG
  PPGQQGTPGTQGLPGPQGAIGPHGEKGPQGKPGLPGMPGSDGPPGHPGKEGPPGTKGNQGPSGPQGPLGY
  PGPRGVKGVDGIRGLKGHKGEKGEDGFPGFKGDIGVKGDRGEVGVPGSRGEDGPEGPKGRTGPTGDPGPP
  GLMGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGASGEKGARGLSGKSGPRGERGPTGPRGQRGPRGATG
  KSGAKGTSGGDGPHGPPGERGLPGPQGPNGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKTGPPGPPGV
  VGPQGAAGETGPMGERGHPGPPGPPGEQGLPGTAGKEGTKGDPGPPGAPGKDGPAGLRGFPGERGLPGTA
  GGPGLKGNEGPSGPPGPAGSPGERGAAGSGGPIGPPGRPGPQGPPGAAGEKGVPGEKGPIGPTGRDGVQG
  PVGLPGPAGPPGVAGEDGDKGEVGDPGQKGTKGNKGEHGPPGPPGPIGPVGQPGAAGADGEPGARGPQGH
  FGAKGDEGTRGFNGPPGPIGLQGLPGPSGEKGETGDVGPMGPPGPPGPRGPAGPNGADGPQGPPGGVGNL
  GPPGEKGEPGESGSPGIQGEPGVKGPRGERGEKGESGQPGEPGPPGPKGPTGDDGPKGNPGPVGFPGDPG
  PPGEGGPRGQDGAKGDRGEDGEPGQPGSPGPTGENGPPGPLGKRGPAGSPGSEGRQGGKGAKGDPGAIGA
  PGKTGPVGPAGPAGKPGPDGLRGLPGSVGQQGRPGATGQAGPPGPVGPPGLPGLRGDAGAKGEKGHPGLI
  GLIGPPGEQGEKGDRGLPGPQGSPGQKGEMGIPGASGPIGPGGPPGLPGPAGPKGAKGATGPGGPKGEKG
  VQGPPGHPGPPGEVIQPLPIQMPKKTRRSVDGSRLMQEDEAIPTGGAPGSPGGLEEIFGSLDSLREEIEQ
  MRRPTGTQDSPARTCQDLKLCHPELPDGEYWVDPNQGCARDAFRVFCNFTAGGETCVTPRDDVTQFSYVD
  SEGSPVGVVQLTFLRLLSVSAHQDVSYPCSGAARDGPLRLRGANEDELSPETSPYVKEFRDGCQTQQGRT
  VLEVRTPVLEQLPVLDASFSDLGAPPRRGGVLLGPVCFMG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080681
• ORF Size: 4950 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080681.2, NP_542412.2
• RefSeq Size: 6167 bp
• RefSeq ORF: 4953 bp
• Locus ID: 1302
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• MW: 162.2 kDa
• Gene Summary: 'This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. It is located on chromosome 6 very close to but separate from the gene for retinoid X receptor beta. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Proteolytic processing of this type XI chain produces PARP, a proline/arginine-rich protein that is an amino terminal domain. Mutations in this gene are associated with type III Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED syndrome), Weissenbacher-Zweymuller syndrome, autosomal dominant non-syndromic sensorineural type 13 deafness (DFNA13), and autosomal recessive non-syndromic sensorineural type 53 deafness (DFNB53). Alternative splicing results in multiple transcript variants. A related pseudogene is located nearby on chromosome 6. [provided by RefSeq, Jul 2009]'