ALG11 (NM_001004127) Human Tagged ORF Clone

CAT#: RC218890

ALG11 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 11, alpha-1,2-mannosyltransferase homolog (yeast) (ALG11), transcript variant A

Product Images

Western blot validation of overexpression lysate (Cat# LY424067) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218890 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALG11
• Synonyms: CDG1P; GT8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218890 representing NM_001004127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGGCGAAAGGAGCTGGTGCCTGTGCAAGTTGTTGAGGTTTTTTTATTCATTATTCTTCCCTG
  GGCTCATTGTATGTGGAACTTTATGTGTGTGTTTGGTCATTGTCCTTTGGGGAATCAGACTGCTGCTACA
  GAGAAAGAAAAAATTAGTGTCAACTAGCAGAAATGGGAAAAATCAAATGGTGATTGCATTTTTTCATCCA
  TACTGCAATGCTGGTGGAGGAGGAGAAAGAGTTTTATGGTGTGCTTTAAGAGCCCTGCAGAAAAAGTATC
  CTGAAGCAGTTTATGTTGTTTATACCGGCGATGTTAATGTCAACGGTCAACAGATACTAGAAGGTGCTTT
  CAGAAGATTTAACATCAGATTAATTCACCCAGTGCAGTTTGTTTTTTTAAGGAAACGCTATCTTGTGGAA
  GATTCACTGTATCCTCACTTCACACTGCTGGGCCAAAGTCTAGGATCCATTTTTCTTGGCTGGGAAGCTC
  TAATGCAGTGTGTTCCTGATGTTTACATTGATTCAATGGGATACGCTTTTACGCTTCCTCTGTTTAAGTA
  TATAGGGGGTTGCCAAGTTGGAAGCTATGTTCATTATCCTACTATCAGCACCGACATGCTCTCTGTAGTG
  AAGAATCAAAATATTGGATTTAATAATGCAGCCTTCATTACCAGGAATCCTTTTCTCAGCAAAGTAAAGC
  TCATCTACTACTATTTATTTGCTTTTATTTATGGACTTGTTGGTTCTTGCAGTGATGTAGTCATGGTCAA
  TTCTTCTTGGACACTAAACCATATTCTCTCACTATGGAAAGTTGGGAATTGCACTAACATTGTTTATCCA
  CCTTGTGATGTGCAGACATTTCTGGACATTCCCTTACATGAGAAAAAGATGACCCCAGGACATTTGCTGG
  TTTCTGTTGGCCAGTTTAGGCCGGAAAAGAATCATCCATTGCAGATCAGAGCCTTTGCTAAATTGCTGAA
  TAAGAAGATGGTTGAGTCACCTCCTTCGCTTAAACTTGTCCTCATTGGAGGTTGTCGTAACAAAGATGAT
  GAACTTAGGGTAAACCAACTGAGAAGGCTGTCTGAGGATTTAGGAGTTCAAGAATATGTGGAATTTAAAA
  TAAACATTCCATTTGATGAATTAAAGAATTATTTGTCTGAAGCAACAATTGGTCTGCATACCATGTGGAA
  CGAGCATTTTGGGATTGGAGTTGTGGAGTGTATGGCAGCTGGCACAATTATCCTTGCACACAATTCGGGG
  GGCCCAAAGCTTGACATTGTGATTCCTCACGAAGGAGATATAACTGGCTTTCTGGCTGAGAGTGAAGAAG
  ACTATGCTGAAACTATCGCTCACATTCTTTCCATGTCTGCAGAAAAGAGACTCCAAATCAGAAAAAGTGC
  TCGTGCATCTGTAAGCAGATTCTCTGATCAGGAATTTGAAGTGACATTCCTATCATCTGTGGAAAAGTTA
  TTTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218890 representing NM_001004127
  Red=Cloning site Green=Tags(s)
  
  MAAGERSWCLCKLLRFFYSLFFPGLIVCGTLCVCLVIVLWGIRLLLQRKKKLVSTSRNGKNQMVIAFFHP
  YCNAGGGGERVLWCALRALQKKYPEAVYVVYTGDVNVNGQQILEGAFRRFNIRLIHPVQFVFLRKRYLVE
  DSLYPHFTLLGQSLGSIFLGWEALMQCVPDVYIDSMGYAFTLPLFKYIGGCQVGSYVHYPTISTDMLSVV
  KNQNIGFNNAAFITRNPFLSKVKLIYYYLFAFIYGLVGSCSDVVMVNSSWTLNHILSLWKVGNCTNIVYP
  PCDVQTFLDIPLHEKKMTPGHLLVSVGQFRPEKNHPLQIRAFAKLLNKKMVESPPSLKLVLIGGCRNKDD
  ELRVNQLRRLSEDLGVQEYVEFKINIPFDELKNYLSEATIGLHTMWNEHFGIGVVECMAAGTIILAHNSG
  GPKLDIVIPHEGDITGFLAESEEDYAETIAHILSMSAEKRLQIRKSARASVSRFSDQEFEVTFLSSVEKL
  FK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004127
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001004127.1, NM_001004127.2, NP_001004127.1, NP_001004127.2
• RefSeq Size: 2585
• RefSeq ORF: 1479
• Locus ID: 440138
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 55.5 kDa
• Gene Summary: This gene encodes a GDP-Man:Man3GlcNAc2-PP-dolichol-alpha1,2-mannosyltransferase which is localized to the cytosolic side of the endoplasmic reticulum (ER) and catalyzes the transfer of the fourth and fifth mannose residue from GDP-mannose (GDP-Man) to Man3GlcNAc2-PP-dolichol and Man4GlcNAc2-PP-dolichol resulting in the production of Man5GlcNAc2-PP-dolichol. Mutations in this gene are associated with congenital disorder of glycosylation type Ip (CDGIP). This gene overlaps but is distinct from the UTP14, U3 small nucleolar ribonucleoprotein, homolog C (yeast) gene. A pseudogene of the GDP-Man:Man3GlcNAc2-PP-dolichol-alpha1,2-mannosyltransferase has been identified on chromosome 19. [provided by RefSeq, Aug 2010]