Glucokinase (GCK) (NM_033508) Human Tagged ORF Clone
CAT#: RC218920L4
- LentiORF®
Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 3, mGFP tagged
"NM_033508" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | GCK |
Synonyms | FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC218920).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_033508 |
ORF Size | 1392 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033508.1, NP_277043.1 |
RefSeq Size | 2566 bp |
RefSeq ORF | 1395 bp |
Locus ID | 2645 |
Cytogenetics | 7p13 |
Protein Families | Druggable Genome |
Protein Pathways | Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus |
MW | 51.9 kDa |
Gene Summary | 'This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC305650 | GCK (untagged)-Human glucokinase (hexokinase 4) (GCK), transcript variant 3 |
USD 780.00 |
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RC218920 | GCK (Myc-DDK-tagged)-Human glucokinase (hexokinase 4) (GCK), transcript variant 3 |
USD 430.00 |
|
RG218920 | GCK (GFP-tagged) - Human glucokinase (hexokinase 4) (GCK), transcript variant 3 |
USD 470.00 |
|
RC218920L1 | Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 3, Myc-DDK-tagged |
USD 630.00 |
|
RC218920L2 | Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 3, mGFP tagged |
USD 630.00 |
|
RC218920L3 | Lenti ORF clone of Human glucokinase (hexokinase 4) (GCK), transcript variant 3, Myc-DDK-tagged |
USD 630.00 |
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