Glucokinase (GCK) (NM_033508) Human Untagged Clone

CAT#: SC305650

GCK (untagged)-Human glucokinase (hexokinase 4) (GCK), transcript variant 3


  "NM_033508" in other vectors (6)

Reconstitution Protocol

USD 780.00

In Stock*

Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GCK
Synonyms FGQTL3; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_033508 edited
ATGCCCAGACCAAGATCCCAACTCCCACAACCCAACTCCCAGGTAGAGCAGATCCTGGCA
GAGTTCCAGCTGCAGGAGGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAGGAGATG
GACCGCGGCCTGAGGCTGGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCCACCTAC
GTGCGCTCCACCCCAGAAGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTGGGTGGC
ACTAACTTCAGGGTGATGCTGGTGAAGGTGGGAGAAGGTGAGGAGGGGCAGTGGAGCGTG
AAGACCAAACACCAGATGTACTCCATCCCCGAGGACGCCATGACCGGCACTGCTGAGATG
CTCTTCGACTACATCTCTGAGTGCATCTCCGACTTCCTGGACAAGCATCAGATGAAACAC
AAGAAGCTGCCCCTGGGCTTCACCTTCTCCTTTCCTGTGAGGCACGAAGACATCGATAAG
GGCATCCTTCTCAACTGGACCAAGGGCTTCAAGGCCTCAGGAGCAGAAGGGAACAATGTC
GTGGGGCTTCTGCGAGACGCTATCAAACGGAGAGGGGACTTTGAAATGGATGTGGTGGCA
ATGGTGAATGACACGGTGGCCACGATGATCTCCTGCTACTACGAAGACCATCAGTGCGAG
GTCGGCATGATCGTGGGCACGGGCTGCAATGCCTGCTACATGGAGGAGATGCAGAATGTG
GAGCTGGTGGAGGGGGACGAGGGCCGCATGTGCGTCAATACCGAGTGGGGCGCCTTCGGG
GACTCCGGCGAGCTGGACGAGTTCCTGCTGGAGTATGACCGCCTGGTGGACGAGAGCTCT
GCAAACCCCGGTCAGCAGCTGTATGAGAAGCTCATAGGTGGCAAGTACATGGGCGAGCTG
GTGCGGCTTGTGCTGCTCAGGCTCGTGGACGAAAACCTGCTCTTCCACGGGGAGGCCTCC
GAGCAGCTGCGCACACGCGGAGCCTTCGAGACGCGCTTCGTGTCGCAGGTGGAGAGCGAC
ACGGGCGACCGCAAGCAGATCTACAACATCCTGAGCACGCTGGGGCTGCGACCCTCGACC
ACCGACTGCGACATCGTGCGCCGCGCCTGCGAGAGCGTGTCTACGCGCGCTGCGCACATG
TGCTCGGCGGGGCTGGCGGGCGTCATCAACCGCATGCGCGAGAGCCGCAGCGAGGACGTA
ATGCGCATCACTGTGGGCGTGGATGGCTCCGTGTACAAGCTGCACCCCAGCTTCAAGGAG
CGGTTCCATGCCAGCGTGCGCAGGCTGACGCCCAGCTGCGAGATCACCTTCATCGAGTCG
GAGGAGGGCAGTGGCCGGGGCGCGGCCCTGGTCTCGGCGGTGGCCTGTAAGAAGGCCTGT
ATGCTGGGCCAGTGAGAGCAGTGGCCGCAAGCGCAGGGAGGATGCCACAGCCCCACAGCA
CCCAGGCTCCATGGGGAAGTGCTCCCCACACGTGCTCGCAGCCTGGCGGGGCAGGAGGCC
TGGCCTTGTCAGGACCCAGGCCGCCTGCCATACCGCTGGGGAACAGAGCGGGCCTCTTCC
CTCAGTTTTTCGGTGGGACAGCCCCAGGGCCCTAACGGGGGTGCGGCAGGAGCAGGAACA
GAGACTCTGGAAGCCCCCCACCTTTCTCGCTGGAATCAATTTCCCAGAAGGGAGTTGCTC
ACTCAGGACTTTGATGCATTTCCACACTGTCAGAGCTGTTGGCCTCGCCTGGGCCCAGGC
TCTGGGAAGGGGTGCCCTCTGGATCCTGCTGTGGCCTCACTTCCCTGGGAACTCATCCTG
TGTGGGGAGGCAGCTCCAACAGCTTGACCAGACCTAGACCTGGGCCAAAAGGGCAGCCAG
GGGCTGCTCATCACCCAGTCCTGGCCATTTTCTTGCCTGAGGCTCAAGAGGCCCAGGGAG
CAATGGGAGGGGGCTCCATGGAGGAGGTGTCCCAAGCTTTGAATACCCCCAGAGACCTTT
TCTCTCCCATACCATCACTGAGTGGCTTGTGATTCTGGGATGGACCCTCGCAGCAGGTGC
AAGAGACAGAGCCCCCAAGCCTCTGCCCCAAGGGGCCCACAAAGGGGAGAAGGGCCAGCC
CTACATCTTCAGCTCCCATAGCGCTGGCTCAGGAAGAAACCCCAAGCAGCATTCAGCACA
CCCCAAGGGACAACCCCATCATATGACATGCCACCCTCTCCATGCCCAACCTAAGATTGT
GTGGGTTTTTTAATTAAAAATGTTAAAAGTTTTAAAAAAAAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_033508 unedited
GTCATATATTTGTATACGACTCACTATAGGCGGCCGCGAATTCATGCCCANACCAANATC
CCAACTCCCACAACCCAACTCCCAGGTAGAGCAGATCCTGGCAGAGTTCCAGCTGCAGGA
GGAGGACCTGAAGAAGGTGATGAGACGGATGCAGAAGGAGATGGACCGCGGCCTGAGGCT
GGAGACCCATGAAGAGGCCAGTGTGAAGATGCTGCCCACCTACGTGCGCTCCACCCCAGA
AGGCTCAGAAGTCGGGGACTTCCTCTCCCTGGACCTGGGTGGCACTAACTTCAGGGTGAT
GCTGGTGAAGGTGGGAGAAGGTGAGGAGGGGCAGTGGAGCGTGAAGACCAAACACCAGAT
GTACTCCATCCCCGAGGACGCCATGACCGGCACTGCTGAGATGCTCTTCGACTACATCTC
TGAGTGCATCTCCGACTTCCTGGACAAGCATCAGATGAAACACAAGAAGCTGCCCCTGGG
CTTCACCTTCTCCTTTCCTGTGAGGCACGAAGACATCGATAAGGGCATCCTTCTCAACTG
GACCAAGGGCTTCAAGGCCTCAGGAGCAGAAGGGAACAATGTCGTGGGGCTTCTGCGAGA
CGCTATCAAACGGAGAGGGGACTTTGAAATGGATGTGGTGGCAATGGTGAATGACACGGT
GGCCACGATGANTCTCTGCTACTACGAAGACCATCAGTGCGAAGTCGGCATGATCGTGGG
CACGGGCTGCAATGCCTGCTACATGGAGGAGATGCANAATGTGGAGCTCGTGGAGGGGGA
CCAGGGCCGCATGTGCGTCAATTCCAATGGGGGCGCCTTTGGGGACTTCGGCGAGCTGAA
CAAGTTCTTGCTGAAGTATTGACCCCCTGGTGGACGAGAGCTTTGCAAACCCCGGTCAGC
AGCTGTATTAAAAAGT
Restriction Sites Please inquire     
ACCN NM_033508
Insert Size 2300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033508.1, NP_277043.1
RefSeq Size 2566 bp
RefSeq ORF 1395 bp
Locus ID 2645
Cytogenetics 7p13
Protein Families Druggable Genome
Protein Pathways Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Maturity onset diabetes of the young, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus
Gene Summary 'This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia. [provided by RefSeq, Aug 2017]'
Transcript Variant: This variant (3) is the minor form expressed in liver. Its first exon is specific to the liver transcripts, variants 2 and 3; its second exon is unique to this transcript. Isoform 3 has a distinct N-terminus; the remainder of the protein is identical to isoforms 1 and 2.

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