EYA3 (NM_001990) Human Tagged ORF Clone

CAT#: RC218948L4

Lenti-ORF clone of EYA3 (mGFP-tagged)-Human eyes absent homolog 3 (Drosophila) (EYA3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: EYA3
• Synonyms: DKFZp686C132
• Vector: pLenti-C-mGFP-P2A-Puro
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: Puromycin
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC218948).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_001990
• ORF Size: 1719 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001990.3, NP_001981.2
• RefSeq Size: 6040 bp
• RefSeq ORF: 1722 bp
• Locus ID: 2140
• Cytogenetics: 1p35.3
• Domains: Hydrolase
• Protein Families: Phosphatase, Transcription Factors
• MW: 62.7 kDa
• Gene Summary: 'This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]'