EYA3 (NM_001990) Human Untagged Clone

CAT#: SC127978

EYA3 (untagged)-Human eyes absent homolog 3 (Drosophila) (EYA3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EYA3
• Synonyms: DKFZp686C132
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001990, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGAAGAGCAAGATTTACCAGAGCAACCAGTGAAAAAAGCCAAGATGCAGGAATCAGGAGAGCAAA
  CTATAAGTCAAGTAAGCAATCCAGATGTCAGTGATCAGAAGCCTGAAACATCAAGCCTTGCTTCAAACCT
  TCCCATGTCAGAGGAAATTATGACATGCACCGATTACATCCCTCGCTCATCCAATGATTATACCTCACAA
  ATGTATTCTGCAAAACCTTATGCACATATTCTCTCAGTTCCTGTTTCGGAAACTGCTTACCCTGGACAGA
  CTCAATACCAGACACTACAGCAGACTCAACCCTATGCTGTCTACCCTCAGGCAACCCAAACGTATGGACT
  ACCTCCTTTTGGTGCATTGTGGCCAGGTATGAAACCTGAAAGTGGTTTAATTCAGACTCCATCTCCAAGT
  CAACACAGTGTTCTTACCTGCACTACAGGGTTAACCACAAGCCAGCCAAGCCCAGCACATTATTCTTATC
  CCATTCAAGCTTCAAGCACAAATGCCAGCCTGATATCTACTTCTTCTACAATTGCCAATATTCCAGCAGC
  AGCAGTAGCCAGCATCTCAAACCAGGATTATCCCACCTATACTATTCTTGGTCAGAATCAGTACCAGGCC
  TGCTACCCCAGCTCCAGCTTTGGAGTCACAGGTCAGACTAACAGTGATGCAGAGAGCACCACATTAGCAG
  CAACCACATACCAGTCGGAGAAGCCTAGTGTCATGGCGCCTGCACCTGCAGCACAGAGACTTTCCTCTGG
  AGACCCTTCTACAAGTCCATCTTTGTCCCAGACTACACCAAGTAAAGATACTGATGATCAGTCCAGGAAA
  AACATGACTAGCAAGAACCGGGGCAAGAGGAAAGCTGATGCCACTTCTTCCCAAGACAGTGAATTAGAAC
  GGGTATTTCTGTGGGACTTGGATGAAACCATCATCATCTTCCACTCACTTCTTACTGGATCCTATGCCCA
  GAAATATGGAAAGGACCCAACAGTAGTGATTGGCTCAGGTTTAACAATGGAAGAAATGATTTTTGAAGTG
  GCTGATACTCATCTATTTTTCAATGACTTAGAGGAGTGTGACCAGGTACATGTGGAAGATGTGGCTTCTG
  ATGACAATGGCCAAGACTTGAGCAACTACAGTTTCTCAACAGATGGTTTCAGTGGCTCAGGAGGTAGTGG
  CAGCCATGGTTCATCTGTGGGTGTTCAGGGAGGTGTGGACTGGATGAGGAAACTAGCTTTCCGCTACCGG
  AAAGTGAGAGAAATCTATGATAAGCATAAAAGCAACGTGGGTGGTCTCCTCAGTCCCCAGAGGAAGGAAG
  CACTGCAGAGATTAAGAGCAGAAATTGAAGTTTTAACAGATTCCTGGTTAGGAACTGCATTAAAGTCCTT
  ACTTCTCATCCAGTCCAGAAAGAATTGTGTGAATGTTCTGATCACTACCACCCAGCTGGTTCCAGCCCTG
  GCCAAGGTTCTCCTATATGGACTAGGAGAAATATTTCCTATTGAGAACATCTATAGTGCTACCAAAATTG
  GTAAGGAGAGCTGCTTTGAGAGAATTGTGTCAAGGTTTGGAAAGAAAGTCACATATGTAGTGATTGGAGA
  TGGACGAGATGAAGAAATTGCAGCCAAACAGCACAACATGCCTTTCTGGAGGATCACAAACCATGGAGAC
  CTAGTATCCCTTCACCAGGCTTTAGAGCTTGATTTTCTCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001990 unedited
  AAAGTACAAATTTTGTAATACGACTCACTATAGGGCGGCCGCATAACTTCGTATAGCATA
  CATTATACGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGCC
  TCCGCTCGATTGGTTCTACTGTGGGTCTGGACTGATCTCCATGTCCTGTTGTGGGGCTTT
  TACAGCCTTTGGATTGTGAAAACTGCTGAGAGAGACTTGCAATCCAGTCACATAAGTATA
  ATAAAGAAATATTGGTCCTCATGGAAGAAGAGCAAGATTTACCAGAGCAACCAGTGAAAA
  AAGCCAAGATGCAGGAATCAGGAGAGCAAACTATAAGTCAAGTAAGCAATCCAGATGTCA
  GTGATCAGAAGCCTGAAACATCAAGCCTTGCTTCAAACCTTCCCATGTCAGAGGAAATTA
  TGACATGCACCGATTACATCCCTCGCTCATCCAATGATTATACCTCACAAATGTATTCTG
  CAAAACCTTATGCACATATTCTCTCAGTTCCTGTTTCGGAAACTGCTTACCCTGGACAGA
  CTCAATACCAGACACTACAGCAGACTCAACCCTATGCTGTCTACCCTCAGGCAACCCAAA
  CGTATGGACTACCTCCTTTTGGTGCATTGTGGCCAGGTATGAAACCTGAAAGTGGTTTAA
  TTCAGACTCCATCTCCAAGTCAACACAGTGTTCTTACCTGCACTACAGGGTTAACCACAA
  GCCAGCCAAGCCCAGCACATTATTCTTATCCCATTCAAGCCTCAAGCACAAATGCCAGCC
  TGATATCTACTTCTTCCTACATTGCCAATATTTCAGCAGCAGCAGTAGCCAGCATCTCAA
  ACCAGGATTATCCCACCTATACTATTNCTTGGTCAGAATCAGTACCAGGCCTGCTACCCC
  AGCTTCAGCTTTGGAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001990
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001990.2, NP_001981.2
• RefSeq Size: 6040 bp
• RefSeq ORF: 1722 bp
• Locus ID: 2140
• Cytogenetics: 1p35.3
• Domains: Hydrolase
• Protein Families: Phosphatase, Transcription Factors
• Gene Summary: 'This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.