MADCAM1 (NM_130760) Human Tagged ORF Clone
CAT#: RC219060
MADCAM1 (Myc-DDK-tagged)-Human mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1
"NM_130760" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MADCAM1 |
Synonyms | MACAM1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219060 representing NM_130760
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTCGGACTGGCCCTCCTGCTGGCGGGGCTTCTGGGGCTCCTCCTCGGCCAGTCCCTCCAGGTGA AGCCCCTGCAGGTGGAGCCCCCGGAGCCGGTGGTGGCCGTGGCCTTGGGCGCCTCGCGCCAGCTCACCTG CCGCCTGGCCTGCGCGGACCGCGGGGCCTCGGTGCAGTGGCGGGGCCTGGACACCAGCCTGGGCGCGGTG CAGTCGGACACGGGCCGCAGCGTCCTCACCGTGCGCAACGCCTCGCTGTCGGCGGCCGGGACCCGCGTGT GCGTGGGCTCCTGCGGGGGCCGCACCTTCCAGCACACCGTGCAGCTCCTTGTGTACGCCTTCCCGGACCA GCTGACCGTCTCCCCGGCAGCCCTGGTGCCTGGTGACCCGGAGGTGGCCTGTACGGCCCACAAAGTCACG CCCGTGGACCCCAACGCGCTCTCCTTCTCCCTGCTCGTCGGGGGCCAGGAACTGGAGGGGGCGCAAGCCC TGGGCCCGGAGGTGCAGGAGGAGGAGGAGGAGCCCCAGGGGGACGAGGACGTGCTGTTCAGGGTGACAGA GCGCTGGCGGCTGCCGCCCCTGGGGACCCCTGTCCCGCCCGCCCTCTACTGCCAGGCCACGATGAGGCTG CCTGGCTTGGAGCTCAGCCACCGCCAGGCCATCCCCGTCCTGCACAGCCCGACCTCCCCGGAGCCTCCCA ACACCACCTCCCCGGAGCCTCCCAACACCACCTCCCCGGAGTCTCCCGACACCACCTCCCCGGAGCCTCC CGACACCACCTCCCCGGAGCCTCCCGACAAGACCTCCCCGGAGCCCGCCCCCCAGCAGGGCTCCACACAC ACCCCCAGGAGCCCAGGCTCCACCAGGACTCGCCGCCCTGAGATCTCCCAGGCTGGGCCCACGCAGGGAG AAGTGATCCCAACAGGCTCGTCCAAACCTGCGGGTGACCAGCTGCCCGCGGCTCTGTGGACCAGCAGTGC GGTGCTGGGACTGCTGCTCCTGGCCTTGCCCACCTATCACCTCTGGAAACGCTGCCGGCACCTGGCTGAG GACGACACCCACCCACCGGCTTCTCTGAGGCTTCTGCCCCAGGTGTCGGCCTGGGCTGGGTTAAGGGGGA CCGGCCAGGTCGGGATCAGCCCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219060 representing NM_130760
Red=Cloning site Green=Tags(s) MDFGLALLLAGLLGLLLGQSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAV QSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVT PVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRL PGLELSHRQAIPVLHSPTSPEPPNTTSPEPPNTTSPESPDTTSPEPPDTTSPEPPDKTSPEPAPQQGSTH TPRSPGSTRTRRPEISQAGPTQGEVIPTGSSKPAGDQLPAALWTSSAVLGLLLLALPTYHLWKRCRHLAE DDTHPPASLRLLPQVSAWAGLRGTGQVGISPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_130760 |
ORF Size | 1146 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_130760.1, NM_130760.2, NP_570116.2 |
RefSeq Size | 1546 bp |
RefSeq ORF | 1149 bp |
Locus ID | 8174 |
Protein Pathways | Cell adhesion molecules (CAMs) |
MW | 38.3 kDa |
Gene Summary | The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC305894 | MADCAM1 (untagged)-Human mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1 |
USD 650.00 |
|
RG219060 | MADCAM1 (GFP-tagged) - Human mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1 |
USD 460.00 |
|
RC219060L1 | Lenti ORF clone of Human mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC219060L2 | Lenti ORF clone of Human mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC219060L3 | Lenti ORF clone of Human mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC219060L4 | Lenti ORF clone of Human mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 1, mGFP tagged |
USD 620.00 |
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