ZP2 (NM_003460) Human Tagged ORF Clone
CAT#: RC219115
ZP2 (Myc-DDK-tagged)-Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2)
"NM_003460" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ZP2 |
Synonyms | OOMD6; Zp-2; ZPA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219115 representing NM_003460
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTGCAGGCAGAGAGGAGGCTCTTGGAGTCCCTCAGGCTGGTTCAATGCAGGCTGGAGCACCTACA GGTCGATTTCTCTCTTCTTCGCCCTTGTGACTTCAGTGAACTCCATAGATGTTTCTCAGTTGGTAAATCC TGCCTTTCCAGGCACTGTCACTTGCGATGAAAGGGAAATAACAGTGGAGTTCCCAAGCAGTCCTGGCACC AAGAAATGGCATGCATCTGTGGTGGATCCTCTTGGTCTCGACATGCCGAACTGCACTTACATCCTGGACC CAGAAAAGCTCACCCTGAGGGCTACCTATGATAACTGTACCAGGAGAGTGCATGGTGGACACCAGATGAC CATCAGAGTCATGAACAACAGTGCTGCCTTAAGACACGGAGCTGTCATGTATCAGTTCTTCTGTCCAGCT ATGCAAGTAGAAGAGACCCAGGGGCTTTCAGCATCTACAATCTGCCAGAAGGATTTCATGTCTTTTTCCT TGCCACGGGTCTTCTCTGGCTTGGCTGATGACAGTAAGGGGACCAAAGTTCAGATGGGATGGAGCATTGA GGTTGGTGATGGTGCAAGAGCCAAAACTCTGACCCTGCCAGAGGCCATGAAGGAAGGCTTCAGCCTCTTG ATTGACAACCACAGGATGACCTTCCATGTGCCATTCAATGCCACTGGAGTGACTCACTATGTGCAAGGTA ACAGTCATCTCTACATGGTGTCTCTGAAGCTTACATTTATATCTCCTGGACAGAAGGTGATCTTCTCTTC ACAAGCTATTTGTGCACCAGATCCTGTGACCTGCAATGCCACACACATGACTCTCACCATACCAGAGTTT CCTGGGAAGCTTAAGTCTGTGAGCTTTGAAAACCAGAACATTGATGTGAGCCAGCTGCATGACAATGGAA TTGATCTAGAAGCAACAAATGGCATGAAATTGCATTTCAGCAAAACTCTGCTCAAAACGAAATTATCTGA AAAATGCCTACTCCATCAGTTCTACTTAGCTTCACTCAAGCTGACCTTTCTCCTTCGGCCAGAGACAGTA TCCATGGTGATCTATCCTGAGTGTCTCTGTGAGTCACCCGTTTCTATAGTTACAGGGGAGCTGTGCACCC AGGATGGGTTTATGGACGTCGAGGTCTACAGCTACCAAACACAACCAGCTCTTGACCTGGGTACTCTGAG GGTGGGAAACTCATCCTGCCAGCCTGTCTTTGAGGCTCAGTCTCAGGGGCTGGTACGGTTCCACATACCC CTGAATGGATGTGGAACGAGATATAAGTTCGAAGATGATAAAGTCGTCTATGAAAACGAAATACATGCTC TCTGGACGGATTTTCCTCCAAGCAAAATATCTAGAGACAGTGAGTTCAGAATGACAGTGAAGTGTTCTTA TAGCAGGAATGACATGCTACTAAACATCAACGTTGAAAGCCTTACTCCTCCAGTGGCCTCAGTGAAGTTG GGTCCATTTACCTTGATCCTGCAAAGCTACCCAGATAATTCCTACCAACAACCTTATGGGGAAAACGAGT ACCCTCTAGTGAGATTCCTCCGCCAACCAATTTACATGGAAGTGAGAGTCCTAAACAGGGATGACCCCAA CATCAAGCTGGTCTTAGATGACTGCTGGGCGACGTCCACCATGGATCCAGACTCTTTCCCCCAGTGGAAC GTTGTCGTGGATGGCTGTGCATATGACCTGGACAACTACCAGACCACCTTCCATCCAGTCGGCTCCTCTG TGACCCATCCTGATCACTATCAGAGGTTTGACATGAAGGCTTTTGCCTTTGTATCAGAAGCCCACGTGCT CTCTAGCCTGGTCTACTTCCACTGCAGTGCCTTAATCTGTAATCGACTCTCCCCTGACTCCCCACTGTGT TCTGTGACCTGCCCTGTGTCCTCTAGGCACAGGCGAGCCACAGGGGCCACTGAAGCAGAGAAAATGACAG TCAGCCTCCCAGGACCCATTCTCCTGTTGTCAGATGACTCCTCATTCAGAGGTGTCGGCTCATCTGATCT AAAAGCAAGTGGGAGCAGTGGGGAGAAGAGTAGGAGTGAAACAGGGGAGGAGGTTGGCTCACGAGGTGCT ATGGACACCAAAGGGCACAAGACTGCTGGAGATGTTGGTTCCAAAGCTGTGGCTGCTGTGGCTGCCTTTG CAGGTGTGGTGGCAACTCTAGGCTTCATCTACTACCTGTACGAGAAAAGGACTGTGTCAAATCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219115 representing NM_003460
Red=Cloning site Green=Tags(s) MACRQRGGSWSPSGWFNAGWSTYRSISLFFALVTSVNSIDVSQLVNPAFPGTVTCDEREITVEFPSSPGT KKWHASVVDPLGLDMPNCTYILDPEKLTLRATYDNCTRRVHGGHQMTIRVMNNSAALRHGAVMYQFFCPA MQVEETQGLSASTICQKDFMSFSLPRVFSGLADDSKGTKVQMGWSIEVGDGARAKTLTLPEAMKEGFSLL IDNHRMTFHVPFNATGVTHYVQGNSHLYMVSLKLTFISPGQKVIFSSQAICAPDPVTCNATHMTLTIPEF PGKLKSVSFENQNIDVSQLHDNGIDLEATNGMKLHFSKTLLKTKLSEKCLLHQFYLASLKLTFLLRPETV SMVIYPECLCESPVSIVTGELCTQDGFMDVEVYSYQTQPALDLGTLRVGNSSCQPVFEAQSQGLVRFHIP LNGCGTRYKFEDDKVVYENEIHALWTDFPPSKISRDSEFRMTVKCSYSRNDMLLNINVESLTPPVASVKL GPFTLILQSYPDNSYQQPYGENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQWN VVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSSLVYFHCSALICNRLSPDSPLC SVTCPVSSRHRRATGATEAEKMTVSLPGPILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGA MDTKGHKTAGDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003460 |
ORF Size | 2235 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003460.1, NM_003460.2, NP_003451.1 |
RefSeq Size | 2266 bp |
RefSeq ORF | 2238 bp |
Locus ID | 7783 |
Domains | zona_pellucida |
Protein Families | Secreted Protein, Transmembrane |
MW | 82.4 kDa |
Gene Summary | The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC111059 | ZP2 (untagged)-Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2) |
USD 1,500.00 |
|
RG219115 | ZP2 (GFP-tagged) - Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2) |
USD 900.00 |
|
RC219115L1 | Lenti ORF clone of Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2), Myc-DDK-tagged |
USD 1,260.00 |
|
RC219115L2 | Lenti ORF clone of Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2), mGFP tagged |
USD 1,020.00 |
|
RC219115L3 | Lenti ORF clone of Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2), Myc-DDK-tagged |
USD 1,020.00 |
|
RC219115L4 | Lenti ORF clone of Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2), mGFP tagged |
USD 1,020.00 |
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