ZP2 (NM_003460) Human Tagged ORF Clone

CAT#: RC219115

ZP2 (Myc-DDK-tagged)-Human zona pellucida glycoprotein 2 (sperm receptor) (ZP2)

Product Images

Western blot validation of overexpression lysate (Cat# LY418662) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219115 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ZP2 protein (Cat# TP319115). The protein was produced from HEK293T cells transfected with ZP2 cDNA clone (Cat# RC219115) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZP2
• Synonyms: OOMD6; Zp-2; ZPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219115 representing NM_003460
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTGCAGGCAGAGAGGAGGCTCTTGGAGTCCCTCAGGCTGGTTCAATGCAGGCTGGAGCACCTACA
  GGTCGATTTCTCTCTTCTTCGCCCTTGTGACTTCAGTGAACTCCATAGATGTTTCTCAGTTGGTAAATCC
  TGCCTTTCCAGGCACTGTCACTTGCGATGAAAGGGAAATAACAGTGGAGTTCCCAAGCAGTCCTGGCACC
  AAGAAATGGCATGCATCTGTGGTGGATCCTCTTGGTCTCGACATGCCGAACTGCACTTACATCCTGGACC
  CAGAAAAGCTCACCCTGAGGGCTACCTATGATAACTGTACCAGGAGAGTGCATGGTGGACACCAGATGAC
  CATCAGAGTCATGAACAACAGTGCTGCCTTAAGACACGGAGCTGTCATGTATCAGTTCTTCTGTCCAGCT
  ATGCAAGTAGAAGAGACCCAGGGGCTTTCAGCATCTACAATCTGCCAGAAGGATTTCATGTCTTTTTCCT
  TGCCACGGGTCTTCTCTGGCTTGGCTGATGACAGTAAGGGGACCAAAGTTCAGATGGGATGGAGCATTGA
  GGTTGGTGATGGTGCAAGAGCCAAAACTCTGACCCTGCCAGAGGCCATGAAGGAAGGCTTCAGCCTCTTG
  ATTGACAACCACAGGATGACCTTCCATGTGCCATTCAATGCCACTGGAGTGACTCACTATGTGCAAGGTA
  ACAGTCATCTCTACATGGTGTCTCTGAAGCTTACATTTATATCTCCTGGACAGAAGGTGATCTTCTCTTC
  ACAAGCTATTTGTGCACCAGATCCTGTGACCTGCAATGCCACACACATGACTCTCACCATACCAGAGTTT
  CCTGGGAAGCTTAAGTCTGTGAGCTTTGAAAACCAGAACATTGATGTGAGCCAGCTGCATGACAATGGAA
  TTGATCTAGAAGCAACAAATGGCATGAAATTGCATTTCAGCAAAACTCTGCTCAAAACGAAATTATCTGA
  AAAATGCCTACTCCATCAGTTCTACTTAGCTTCACTCAAGCTGACCTTTCTCCTTCGGCCAGAGACAGTA
  TCCATGGTGATCTATCCTGAGTGTCTCTGTGAGTCACCCGTTTCTATAGTTACAGGGGAGCTGTGCACCC
  AGGATGGGTTTATGGACGTCGAGGTCTACAGCTACCAAACACAACCAGCTCTTGACCTGGGTACTCTGAG
  GGTGGGAAACTCATCCTGCCAGCCTGTCTTTGAGGCTCAGTCTCAGGGGCTGGTACGGTTCCACATACCC
  CTGAATGGATGTGGAACGAGATATAAGTTCGAAGATGATAAAGTCGTCTATGAAAACGAAATACATGCTC
  TCTGGACGGATTTTCCTCCAAGCAAAATATCTAGAGACAGTGAGTTCAGAATGACAGTGAAGTGTTCTTA
  TAGCAGGAATGACATGCTACTAAACATCAACGTTGAAAGCCTTACTCCTCCAGTGGCCTCAGTGAAGTTG
  GGTCCATTTACCTTGATCCTGCAAAGCTACCCAGATAATTCCTACCAACAACCTTATGGGGAAAACGAGT
  ACCCTCTAGTGAGATTCCTCCGCCAACCAATTTACATGGAAGTGAGAGTCCTAAACAGGGATGACCCCAA
  CATCAAGCTGGTCTTAGATGACTGCTGGGCGACGTCCACCATGGATCCAGACTCTTTCCCCCAGTGGAAC
  GTTGTCGTGGATGGCTGTGCATATGACCTGGACAACTACCAGACCACCTTCCATCCAGTCGGCTCCTCTG
  TGACCCATCCTGATCACTATCAGAGGTTTGACATGAAGGCTTTTGCCTTTGTATCAGAAGCCCACGTGCT
  CTCTAGCCTGGTCTACTTCCACTGCAGTGCCTTAATCTGTAATCGACTCTCCCCTGACTCCCCACTGTGT
  TCTGTGACCTGCCCTGTGTCCTCTAGGCACAGGCGAGCCACAGGGGCCACTGAAGCAGAGAAAATGACAG
  TCAGCCTCCCAGGACCCATTCTCCTGTTGTCAGATGACTCCTCATTCAGAGGTGTCGGCTCATCTGATCT
  AAAAGCAAGTGGGAGCAGTGGGGAGAAGAGTAGGAGTGAAACAGGGGAGGAGGTTGGCTCACGAGGTGCT
  ATGGACACCAAAGGGCACAAGACTGCTGGAGATGTTGGTTCCAAAGCTGTGGCTGCTGTGGCTGCCTTTG
  CAGGTGTGGTGGCAACTCTAGGCTTCATCTACTACCTGTACGAGAAAAGGACTGTGTCAAATCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219115 representing NM_003460
  Red=Cloning site Green=Tags(s)
  
  MACRQRGGSWSPSGWFNAGWSTYRSISLFFALVTSVNSIDVSQLVNPAFPGTVTCDEREITVEFPSSPGT
  KKWHASVVDPLGLDMPNCTYILDPEKLTLRATYDNCTRRVHGGHQMTIRVMNNSAALRHGAVMYQFFCPA
  MQVEETQGLSASTICQKDFMSFSLPRVFSGLADDSKGTKVQMGWSIEVGDGARAKTLTLPEAMKEGFSLL
  IDNHRMTFHVPFNATGVTHYVQGNSHLYMVSLKLTFISPGQKVIFSSQAICAPDPVTCNATHMTLTIPEF
  PGKLKSVSFENQNIDVSQLHDNGIDLEATNGMKLHFSKTLLKTKLSEKCLLHQFYLASLKLTFLLRPETV
  SMVIYPECLCESPVSIVTGELCTQDGFMDVEVYSYQTQPALDLGTLRVGNSSCQPVFEAQSQGLVRFHIP
  LNGCGTRYKFEDDKVVYENEIHALWTDFPPSKISRDSEFRMTVKCSYSRNDMLLNINVESLTPPVASVKL
  GPFTLILQSYPDNSYQQPYGENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQWN
  VVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSSLVYFHCSALICNRLSPDSPLC
  SVTCPVSSRHRRATGATEAEKMTVSLPGPILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGA
  MDTKGHKTAGDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003460
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003460.1, NM_003460.2, NP_003451.1
• RefSeq Size: 2266 bp
• RefSeq ORF: 2238 bp
• Locus ID: 7783
• Domains: zona_pellucida
• Protein Families: Secreted Protein, Transmembrane
• MW: 82.4 kDa
• Gene Summary: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]