SARDH (NM_007101) Human Tagged ORF Clone

CAT#: RC219173

SARDH (Myc-DDK-tagged)-Human sarcosine dehydrogenase (SARDH), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY416196) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219173 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SARDH
• Synonyms: BPR-2; DMGDHL1; SAR; SARD; SDH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219173 representing NM_007101
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCACTGAGCCGAGCCCTACGTGTGGCTGCTGCCCACCCTCGCCAGAGCCCTACCCGGGGCATGG
  GGCCATGCAACCTGTCCAGCGCAGCTGGCCCCACAGCCGAGAAGAGTGTGCCATATCAGCGGACCCTGAA
  GGAGGGACAGGGCACCTCGGTGGTGGCCCAAGGCCCAAGCCGGCCCCTGCCCAGCACGGCCAACGTGGTG
  GTCATTGGTGGAGGCAGCTTGGGCTGCCAGACCCTGTACCACCTGGCCAAGCTGGGCATGAGTGGGGCGG
  TGCTGCTGGAGCGGGAGCGGCTGACCTCCGGGACCACCTGGCACACGGCAGGCCTGCTGTGGCAGCTGCG
  GCCCAGTGACGTGGAGGTGGAGCTTCTGGCCCACACTCGGCGGGTGGTGAGCCGGGAGCTGGAGGAGGAG
  ACGGGACTACACACGGGCTGGATCCAGAATGGGGGCCTCTTCATCGCGTCCAACCGGCAGCGCCTGGACG
  AGTACAAGAGGCTCATGTCGCTGGGCAAGGCGTATGGTGTGGAATCCCATGTGCTGAGCCCGGCAGAGAC
  CAAGACTCTGTACCCGCTGATGAATGTGGACGACCTCTACGGGACCCTGTATGTGCCGCACGACGGTACC
  ATGGACCCCGCTGGCACCTGTACCACCCTCGCCAGGGCAGCTTCTGCCCGAGGAGCACAGGTCATTGAGA
  ACTGCCCAGTGACCGGCATTCGTGTGTGGACGGATGATTTTGGGGTGCGGCGGGTCGCGGGTGTGGAGAC
  TCAGCATGGTTCCATCCAGACACCCTGCGTGGTCAACTGTGCAGGAGTGTGGGCAAGTGCTGTGGGCCGG
  ATGGCTGGAGTCAAGGTCCCGCTGGTGGCCATGCACCATGCCTATGTCGTCACCGAGCGCATCGAGGGGA
  TTCAGAACATGCCCAATGTCCGTGATCATGATGCCTCTGTCTACCTCCGCCTCCAAGGGGATGCCTTGTC
  TGTGGGTGGCTATGAGGCCAACCCCATCTTTTGGGAGGAGGTGTCAGACAAGTTTGCCTTCGGCCTCTTT
  GACCTGGACTGGGAGGTGTTCACCCAGCACATTGAAGGCGCCATCAACAGGGTCCCCGTGCTGGAGAAGA
  CAGGAATCAAGTCCACGGTCTGCGGCCCTGAATCCTTCACGCCCGACCACAAGCCCCTGATGGGGGAGGC
  ACCTGAGCTCCGAGGGTTCTTCCTGGGCTGTGGCTTCAACAGCGCAGGAATGATGCTGGGTGGTGGCTGT
  GGGCAGGAGCTGGCCCACTGGATCATCCATGGGCGCCCGGAGAAGGACATGCATGGCTATGACATCAGGC
  GCTTCCATCACTCGCTCACGGACCACCCCCGCTGGATCCGAGAGCGAAGCCATGAGTCCTACGCCAAGAA
  CTACTCCGTCGTCTTCCCCCACGATGAGCCGCTGGCCGGGCGCAACATGAGGAGAGACCCGCTGCACGAG
  GAACTCCTTGGACAAGGCTGCGTGTTCCAGGAGCGGCATGGCTGGGAGCGACCGGGATGGTTTCATCCCC
  GAGGCCCAGCTCCGGTCCTCGAGTACGACTACTACGGGGCTTACGGGAGCCGCGCGCACGAGGACTACGC
  CTACCGCAGGCTGCTGGCAGACGAGTACACCTTCGCCTTCCCGCCCCACCACGACACGATCAAGAAGGAG
  TGCCTGGCCTGCAGAGGGGCCGCCGCTGTGTTTGACATGTCCTACTTCGGGAAGTTCTACCTGGTGGGGC
  TGGATGCAAGGAAGGCTGCCGACTGGCTCTTCTCCGCAGATGTCAGCCGACCCCCAGGCTCCACCGTGTA
  CACGTGCATGCTCAACCACCGTGGGGGCACCGAGAGTGACCTGACTGTCAGCCGCCTGGCACCCAGCCAC
  CAGGCCTCCCCGCTGGCCCCCGCCTTTGAAGGGGACGGTTACTACCTGGCCATGGGCGGGGCCGTGGCCC
  AGCACAACTGGTCCCACATCACCACCGTGCTGCAGGACCAGAAGTCCCAGTGCCAGCTCATCGACAGCTC
  CGAGGACCTGGGTATGATCAGTATCCAGGGCCCAGCCAGCCGAGCCATTTTGCAGGAGGTGCTGGACGCA
  GACCTGAGCAACGAGGCCTTCCCGTTCTCCACCCACAAGCTACTGAGAGCCGCAGGGCACCTGGTCCGAG
  CCATGCGGCTGTCCTTTGTGGGGGAGCTGGGCTGGGAGCTGCACATTCCAAAGGCGTCCTGCGTGCCTGT
  GTACCGGGCTGTGATGGCCGCGGGTGCCAAGCACGGCCTCATCAACGCAGGGTACCGCGCCATCGACTCC
  CTGAGCATTGAGAAAGGCTACCGGCACTGGCACGCGGACCTGCGGCCAGACGACAGCCCCCTGGAGGCAG
  GCCTGGCCTTCACCTGCAAGCTCAAGTCGCCGGTGCCCTTCCTGGGGAGGGAGGCCCTGGAGCAGCAGCG
  GGCCGCAGGCCTCCGCCGGCGCCTGGTGTGCTTCACCATGGAGGACAAAGTACCCATGTTTGGCCTGGAG
  GCCATCTGGAGGAACGGCCAAGTGGTGGGCCATGTCCGGAGGGCTGACTTTGGGTTCGCCATCGACAAGA
  CCATCGCCTACGGTTACATCCATGACCCCAGCGGTGGGCCGGTCTCGCTGGACTTTGTGAAGAGCGGGGA
  CTATGCCCTGGAGAGAATGGGGGTGACCTATGGTGCCCAGGCTCACCTGAAGTCGCCCTTCGACCCCAAC
  AACAAGAGGGTGAAGGGAATCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219173 representing NM_007101
  Red=Cloning site Green=Tags(s)
  
  MASLSRALRVAAAHPRQSPTRGMGPCNLSSAAGPTAEKSVPYQRTLKEGQGTSVVAQGPSRPLPSTANVV
  VIGGGSLGCQTLYHLAKLGMSGAVLLERERLTSGTTWHTAGLLWQLRPSDVEVELLAHTRRVVSRELEEE
  TGLHTGWIQNGGLFIASNRQRLDEYKRLMSLGKAYGVESHVLSPAETKTLYPLMNVDDLYGTLYVPHDGT
  MDPAGTCTTLARAASARGAQVIENCPVTGIRVWTDDFGVRRVAGVETQHGSIQTPCVVNCAGVWASAVGR
  MAGVKVPLVAMHHAYVVTERIEGIQNMPNVRDHDASVYLRLQGDALSVGGYEANPIFWEEVSDKFAFGLF
  DLDWEVFTQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSAGMMLGGGC
  GQELAHWIIHGRPEKDMHGYDIRRFHHSLTDHPRWIRERSHESYAKNYSVVFPHDEPLAGRNMRRDPLHE
  ELLGQGCVFQERHGWERPGWFHPRGPAPVLEYDYYGAYGSRAHEDYAYRRLLADEYTFAFPPHHDTIKKE
  CLACRGAAAVFDMSYFGKFYLVGLDARKAADWLFSADVSRPPGSTVYTCMLNHRGGTESDLTVSRLAPSH
  QASPLAPAFEGDGYYLAMGGAVAQHNWSHITTVLQDQKSQCQLIDSSEDLGMISIQGPASRAILQEVLDA
  DLSNEAFPFSTHKLLRAAGHLVRAMRLSFVGELGWELHIPKASCVPVYRAVMAAGAKHGLINAGYRAIDS
  LSIEKGYRHWHADLRPDDSPLEAGLAFTCKLKSPVPFLGREALEQQRAAGLRRRLVCFTMEDKVPMFGLE
  AIWRNGQVVGHVRRADFGFAIDKTIAYGYIHDPSGGPVSLDFVKSGDYALERMGVTYGAQAHLKSPFDPN
  NKRVKGIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007101
• ORF Size: 2754 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007101.2, NM_007101.3, NP_009032.2
• RefSeq Size: 3363 bp
• RefSeq ORF: 2757 bp
• Locus ID: 1757
• Cytogenetics: 9q34.2
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways
• MW: 101.04 kDa
• Gene Summary: 'This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2008]'