SARDH (NM_007101) Human Untagged Clone

CAT#: SC303875

SARDH (untagged)-Human sarcosine dehydrogenase (SARDH), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SARDH
• Synonyms: BPR-2; DMGDHL1; SAR; SARD; SDH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007101, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCTCACTGAGCCGAGCCCTACGTGTGGCTGCTGCCCACCCTCGCCAGAGCCCTACCCGGGGCATGG
  GGCCATGCAACCTGTCCAGCGCAGCTGGCCCCACAGCCGAGAAGAGTGTGCCATATCAGCGGACCCTGAA
  GGAGGGACAGGGCACCTCGGTGGTGGCCCAAGGCCCAAGCCGGCCCCTGCCCAGCACGGCCAACGTGGTG
  GTCATTGGTGGAGGCAGCTTGGGCTGCCAGACCCTGTACCACCTGGCCAAGCTGGGCATGAGTGGGGCGG
  TGCTGCTGGAGCGGGAGCGGCTGACCTCCGGGACCACCTGGCACACGGCAGGCCTGCTGTGGCAGCTGCG
  GCCCAGTGACGTGGAGGTGGAGCTTCTGGCCCACACTCGGCGGGTGGTGAGCCGGGAGCTGGAGGAGGAG
  ACGGGACTACACACGGGCTGGATCCAGAATGGGGGCCTCTTCATCGCGTCCAACCGGCAGCGCCTGGACG
  AGTACAAGAGGCTCATGTCGCTGGGCAAGGCGTATGGTGTGGAATCCCATGTGCTGAGCCCGGCAGAGAC
  CAAGACTCTGTACCCGCTGATGAATGTGGACGACCTCTACGGGACCCTGTATGTGCCGCACGACGGTACC
  ATGGACCCCGCTGGCACCTGTACCACCCTCGCCAGGGCAGCTTCTGCCCGAGGAGCACAGGTCATTGAGA
  ACTGCCCAGTGACCGGCATTCGTGTGTGGACGGATGATTTTGGGGTGCGGCGGGTCGCGGGTGTGGAGAC
  TCAGCATGGTTCCATCCAGACACCCTGCGTGGTCAACTGTGCAGGAGTGTGGGCAAGTGCTGTGGGCCGG
  ATGGCTGGAGTCAAGGTCCCGCTGGTGGCCATGCACCATGCCTATGTCGTCACCGAGCGCATCGAGGGGA
  TTCAGAACATGCCCAATGTCCGTGATCATGATGCCTCTGTCTACCTCCGCCTCCAAGGGGATGCCTTGTC
  TGTGGGTGGCTATGAGGCCAACCCCATCTTTTGGGAGGAGGTGTCAGACAAGTTTGCCTTCGGCCTCTTT
  GACCTGGACTGGGAGGTGTTCACCCAGCACATTGAAGGCGCCATCAACAGGGTCCCCGTGCTGGAGAAGA
  CAGGAATCAAGTCCACGGTCTGCGGCCCTGAATCCTTCACGCCCGACCACAAGCCCCTGATGGGGGAGGC
  ACCTGAGCTCCGAGGGTTCTTCCTGGGCTGTGGCTTCAACAGCGCAGGAATGATGCTGGGTGGTGGCTGT
  GGGCAGGAGCTGGCCCACTGGATCATCCATGGGCGCCCGGAGAAGGACATGCATGGCTATGACATCAGGC
  GCTTCCATCACTCGCTCACGGACCACCCCCGCTGGATCCGAGAGCGAAGCCATGAGTCCTACGCCAAGAA
  CTACTCCGTCGTCTTCCCCCACGATGAGCCGCTGGCCGGGCGCAACATGAGGAGAGACCCGCTGCACGAG
  GAACTCCTTGGACAAGGCTGCGTGTTCCAGGAGCGGCATGGCTGGGAGCGACCGGGATGGTTTCATCCCC
  GAGGCCCAGCTCCGGTCCTCGAGTACGACTACTACGGGGCTTACGGGAGCCGCGCGCACGAGGACTACGC
  CTACCGCAGGCTGCTGGCAGACGAGTACACCTTCGCCTTCCCGCCCCACCACGACACGATCAAGAAGGAG
  TGCCTGGCCTGCAGAGGGGCCGCCGCTGTGTTTGACATGTCCTACTTCGGGAAGTTCTACCTGGTGGGGC
  TGGATGCAAGGAAGGCTGCCGACTGGCTCTTCTCCGCAGATGTCAGCCGACCCCCAGGCTCCACCGTGTA
  CACGTGCATGCTCAACCACCGTGGGGGCACCGAGAGTGACCTGACTGTCAGCCGCCTGGCACCCAGCCAC
  CAGGCCTCCCCGCTGGCCCCCGCCTTTGAAGGGGACGGTTACTACCTGGCCATGGGCGGGGCCGTGGCCC
  AGCACAACTGGTCCCACATCACCACCGTGCTGCAGGACCAGAAGTCCCAGTGCCAGCTCATCGACAGCTC
  CGAGGACCTGGGTATGATCAGTATCCAGGGCCCAGCCAGCCGAGCCATTTTGCAGGAGGTGCTGGACGCA
  GACCTGAGCAACGAGGCCTTCCCGTTCTCCACCCACAAGCTACTGAGAGCCGCAGGGCACCTGGTCCGAG
  CCATGCGGCTGTCCTTTGTGGGGGAGCTGGGCTGGGAGCTGCACATTCCAAAGGCGTCCTGCGTGCCTGT
  GTACCGGGCTGTGATGGCCGCGGGTGCCAAGCACGGCCTCATCAACGCAGGGTACCGCGCCATCGACTCC
  CTGAGCATTGAGAAAGGCTACCGGCACTGGCACGCGGACCTGCGGCCAGACGACAGCCCCCTGGAGGCAG
  GCCTGGCCTTCACCTGCAAGCTCAAGTCGCCGGTGCCCTTCCTGGGGAGGGAGGCCCTGGAGCAGCAGCG
  GGCCGCAGGCCTCCGCCGGCGCCTGGTGTGCTTCACCATGGAGGACAAAGTACCCATGTTTGGCCTGGAG
  GCCATCTGGAGGAACGGCCAAGTGGTGGGCCATGTCCGGAGGGCTGACTTTGGGTTCGCCATCGACAAGA
  CCATCGCCTACGGTTACATCCATGACCCCAGCGGTGGGCCGGTCTCGCTGGACTTTGTGAAGAGCGGGGA
  CTATGCCCTGGAGAGAATGGGGGTGACCTATGGTGCCCAGGCTCACCTGAAGTCGCCCTTCGACCCCAAC
  AACAAGAGGGTGAAGGGAATCTACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_007101
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007101.3, NP_009032.2
• RefSeq Size: 3398 bp
• RefSeq ORF: 2757 bp
• Locus ID: 1757
• Cytogenetics: 9q34.2
• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2008]'
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same isoform.