SAP102 (DLG3) (NM_020730) Human Tagged ORF Clone

CAT#: RC219249

DLG3 (Myc-DDK-tagged)-Human discs, large homolog 3 (Drosophila) (DLG3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DLG3
• Synonyms: MRX; MRX90; NEDLG; PPP1R82; SAP102; XLMR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219249 representing NM_020730
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGGCCCGCAAGTTCTCGGGCTCCGGCTTGGCCATGGGCTTGGGCTCCGCCTCCGCTTCGGCCT
  GGAGGAGGGCTTCGCAGAGGTGGGCCTGGCCGCTCCGCTCCCTGCGGCCCGGAGGGGATGCCAGAGAGCC
  TCGCAAGATCATCCTGCACAAAGGCTCCACAGGCCTGGGCTTCAACATCGTAGGAGGAGAGGATGGAGAA
  GGCATTTTTGTCTCCTTCATCCTGGCAGGAGGCCCAGCTGACCTGAGTGGGGAGCTGCGCAGGGGAGACC
  GGATCTTATCGGTGAATGGAGTGAATCTGAGGAATGCAACTCATGAGCAGGCTGCAGCTGCTCTGAAACG
  GGCCGGCCAGTCAGTCACCATTGTGGCCCAGTACAGACCTGAAGAATACAGTCGCTTTGAATCGAAGATA
  CATGACTTACGAGAACAAATGATGAACAGCAGCATGAGCTCTGGGTCTGGGTCCCTCCGAACAAGTGAAA
  AGAGGTCCTTGTATGTCAGGGCCCTGTTTGATTATGATCGGACTCGGGACAGCTGCCTGCCAAGCCAGGG
  GCTCAGCTTCTCTTATGGTGACATTCTGCATGTCATTAATGCCTCTGATGATGAGTGGTGGCAGGCAAGG
  CTGGTGACCCCACACGGAGAAAGTGAGCAGATCGGTGTGATCCCCAGTAAGAAGAGGGTGGAAAAGAAAG
  AAAGAGCTCGATTGAAAACTGTGAAGTTCCATGCCAGGACGGGGATGATTGAGTCTAACAGGTCGATCAA
  AACGAAACGTAAAAAGAGTTTCCGCCTCTCTCGAAAGTTTCCATTTTACAAGAGCAAAGAAAACATGGCC
  CAGGAGAGCAGCATACAGGAACAGGGAGTGACATCCAACACCAGTGACAGCGAAAGCAGTTCCAAAGGAC
  AAGAGGATGCTATTTTGTCATATGAGCCAGTGACACGGCAAGAAATTCACTATGCAAGGCCTGTGATCAT
  CCTGGGCCCAATGAAGGACCGAGTCAATGATGACCTGATCTCCGAATTTCCACATAAATTTGGATCCTGT
  GTGCCACATACTACCCGGCCTCGACGTGATAATGAGGTGGATGGACAAGACTACCACTTTGTGGTGTCCC
  GAGAACAAATGGAGAAAGATATTCAGGACAACAAGTTCATCGAGGCGGGCCAATTTAATGATAACCTCTA
  TGGGACCAGCATCCAGTCAGTGCGGGCAGTTGCAGAGAGGGGCAAGCACTGCATCTTAGATGTTTCCGGC
  AATGCTATCAAGAGACTGCAGCAAGCACAACTTTACCCCATTGCCATTTTCATCAAGCCCAAGTCCATTG
  AAGCCCTTATGGAAATGAACCGAAGGCAGACATATGAACAAGCAAATAAGATCTATGACAAAGCCATGAA
  ACTGGAGCAGGAATTTGGAGAGTACTTTACAGCCATTGTACAGGGTGACTCACTGGAAGAGATTTATAAC
  AAAATCAAACAAATCATTGAGGACCAGTCTGGGCACTACATTTGGGTCCCATCCCCTGAAAAACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219249 representing NM_020730
  Red=Cloning site Green=Tags(s)
  
  MERARKFSGSGLAMGLGSASASAWRRASQRWAWPLRSLRPGGDAREPRKIILHKGSTGLGFNIVGGEDGE
  GIFVSFILAGGPADLSGELRRGDRILSVNGVNLRNATHEQAAAALKRAGQSVTIVAQYRPEEYSRFESKI
  HDLREQMMNSSMSSGSGSLRTSEKRSLYVRALFDYDRTRDSCLPSQGLSFSYGDILHVINASDDEWWQAR
  LVTPHGESEQIGVIPSKKRVEKKERARLKTVKFHARTGMIESNRSIKTKRKKSFRLSRKFPFYKSKENMA
  QESSIQEQGVTSNTSDSESSSKGQEDAILSYEPVTRQEIHYARPVIILGPMKDRVNDDLISEFPHKFGSC
  VPHTTRPRRDNEVDGQDYHFVVSREQMEKDIQDNKFIEAGQFNDNLYGTSIQSVRAVAERGKHCILDVSG
  NAIKRLQQAQLYPIAIFIKPKSIEALMEMNRRQTYEQANKIYDKAMKLEQEFGEYFTAIVQGDSLEEIYN
  KIKQIIEDQSGHYIWVPSPEKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020730
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020730.1, NM_020730.2, NP_065781.1
• RefSeq Size: 5068 bp
• RefSeq ORF: 1539 bp
• Locus ID: 1741
• Cytogenetics: Xq13.1
• MW: 57.8 kDa
• Gene Summary: 'This gene encodes a member of the membrane-associated guanylate kinase protein family. The encoded protein may play a role in clustering of NMDA receptors at excitatory synapses. It may also negatively regulate cell proliferation through interaction with the C-terminal region of the adenomatosis polyposis coli tumor suppressor protein. Mutations in this gene have been associated with X-linked cognitive disability. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]'