MEST (NM_177525) Human Tagged ORF Clone
CAT#: RC219273L3
- LentiORF®
Lenti-ORF clone of MEST (Myc-DDK-tagged)-Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 3
"NM_177525" in other vectors (4)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MEST |
Synonyms | PEG1 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC219273).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_177525 |
ORF Size | 978 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177525.1, NP_803491.1 |
RefSeq Size | 2419 bp |
RefSeq ORF | 981 bp |
Locus ID | 4232 |
Cytogenetics | 7q32.2 |
Protein Families | Protease, Transmembrane |
MW | 37.4 kDa |
Gene Summary | 'This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC109431 | MEST (untagged)-Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 3 |
USD 660.00 |
|
RC219273 | MEST (Myc-DDK-tagged)-Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 3 |
USD 420.00 |
|
RG219273 | MEST (GFP-tagged) - Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 3 |
USD 460.00 |
|
RC219273L4 | Lenti-ORF clone of MEST (mGFP-tagged)-Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 3 |
USD 620.00 |
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