MEST (NM_177525) Human Untagged Clone

CAT#: SC109431

MEST (untagged)-Human mesoderm specific transcript homolog (mouse) (MEST), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MEST
• Synonyms: PEG1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109431 sequence for NM_177525 edited (data generated by NextGen Sequencing)
  ATGAGGGAGTGGTGGGTCCAGGTGGGGCTGCTGGCCGTGCCCCTGCTTGCTGCGTACCTG
  CACATCCCACCCCCTCAGCTCTCCCCTGCCCTTCACTCATGGAAGTCTTCAGGCAAGTTT
  TTCACTTACAAGGGACTGCGTATCTTCTACCAAGACTCTGTGGGTGTGGTTGGAAGTCCA
  GAGATAGTTGTGCTTTTACACGGTTTTCCAACATCCAGCTACGACTGGTACAAGATTTGG
  GAAGGTCTGACCTTGAGGTTTCATCGGGTGATTGCCCTTGATTTCTTAGGCTTTGGCTTC
  AGTGACAAACCGAGACCACATCACTATTCCATATTTGAGCAGGCCAGCATCGTGGAAGCG
  CTTTTGCGGCATCTGGGGCTCCAGAACCGCAGGATCAACCTTCTTTCTCATGACTATGGA
  GATATTGTTGCTCAGGAGCTTCTCTACAGGTACAAGCAGAATCGATCTGGTCGGCTTACC
  ATAAAGAGTCTCTGTCTGTCAAATGGAGGTATCTTTCCTGAGACTCACCGTCCACTCCTT
  CTCCAAAAGCTACTCAAAGATGGAGGTGTGCTGTCACCCATCCTCACACGACTGATGAAC
  TTCTTTGTATTCTCTCGAGGTCTCACCCCAGTCTTTGGGCCGTATACTCGGCCCTCTGAG
  AGTGAGCTGTGGGACATGTGGGCAGGGATCCGCAACAATGACGGGAACTTAGTCATTGAC
  AGTCTCTTACAGTACATCAATCAGAGGAAGAAGTTCAGAAGGCGCTGGGTGGGAGCTCTT
  GCCTCTGTAACTATCCCCATTCATTTTATCTATGGGCCATTGGATCCTGTAAATCCCTAT
  CCAGAGTTTTTGGAGCTGTACAGGAAAACGCTGCCGCGGTCCACAGTGTCGATTCTGGAT
  GACCACATTAGCCACTATCCACAGCTAGAGGATCCCATGGGCTTCTTGAATGCATATATG
  GGCTTCATCAACTCCTTCTGA
  
  Clone variation with respect to NM_177525.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_177525 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGTAGGCAAGGTCTTAC
  CTGAATCAGGATGAGGGAGTGGTGGGTCCAGGTGGGGCTGCTGGCCGTGCCCCTGCTTGC
  TGCGTACCTGCACATCCCACCCCCTCAGCTCTCCCCTGCCCTTCACTCATGGAAGTCTTC
  AGGCAAGTTTTTCACTTACAAGGGACTGCGTATCTTCTACCAAGACTCTGTGGGTGTGGT
  TGGAAGTCCAGAGATAGTTGTGCTTTTACACGGTTTTCCAACATCCAGCTACGACTGGTA
  CAAGATTTGGGAAGGTCTGACCTTGAGGTTTCATCGGGTGATTGCCCTTGATTTCTTAGG
  CTTTGGCTTCAGTGACAAACCGAGACCACATCACTATTCCATATTTGAGCAGGCCAGCAT
  CGTGGAAGCGCTTTTGCGGCATCTGGGGCTCCAGAACCGCAGGATCAACCTTCTTTCTCA
  TGACTATGGAGATATTGTTGCTCAGGAGCTTCTCTACAGGTACAAGCAGAATCGATCTGG
  TCGGCTTACCATAAAGAGTCTCTGTCTGTCAAATGGAGGTATCTTTCCTGAGACTCACCG
  TCCACTCCTTCTCCAAAAGCTACTCAAAGATGGAGGTGTGCTGTCACCCATCCTCACACG
  ACTGATGAACTTCTTTGTATTCTCTCGAGGTCTCACCCCAGTCTTTGGGCCGTATACTCG
  GCCCTCTGAGAGTGAGCTGTGGGACATGGGGGCAGGGATCCGCAACAATGACGGGAACTT
  AGTCATTGACAGTCTCTTACAGTACATCAATCAGAGGAAGAAAGTCAGAAGGCGCTNGGT
  GGGAGCTCTTGCCTCTGNNTACTATCCCATTCATTTTATCTATGGCCATTGGATCCTGTA
  ACTCCCTATNCANAGTTTGGNAGCTGACAGAAAACGCTGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_177525
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177525.1, NP_803491.1
• RefSeq Size: 2419 bp
• RefSeq ORF: 981 bp
• Locus ID: 4232
• Cytogenetics: 7q32.2
• Protein Families: Protease, Transmembrane
• Gene Summary: 'This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011]'
  Transcript Variant: This variant (3) differs in the 5' UTR and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (b), which has a shorter N-terminus compared to isoform a. This isoform (b) is biallelically expressed.