Mannose Receptor (MRC1) (NM_002438) Human Tagged ORF Clone

CAT#: RC219343

MRC1 (Myc-DDK-tagged)-Human mannose receptor, C type 1 (MRC1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MRC1
• Synonyms: bA541I19.1; CD206; CLEC13D; CLEC13DL; hMR; MMR; MRC1L1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219343 representing NM_002438
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTACCCCTGCTCCTGGTTTTTGCCTCTGTCATTCCGGGTGCTGTTCTCCTACTGGACACCAGGC
  AATTTTTAATCTATAATGAAGATCACAAGCGCTGCGTGGATGCAGTGAGTCCCAGTGCCGTCCAAACCGC
  AGCTTGCAACCAGGATGCCGAATCACAGAAATTCCGATGGGTGTCCGAATCTCAGATTATGAGTGTTGCA
  TTTAAATTATGCCTGGGAGTGCCATCAAAAACAGACTGGGTTGCTATCACTCTCTATGCCTGTGACTCAA
  AAAGTGAATTTCAGAAATGGGAGTGCAAAAATGACACACTTTTGGGGATCAAAGGAGAAGATTTATTTTT
  TAACTACGGCAACAGACAAGAAAAGAATATTATGCTCTACAAGGGATCGGGTTTATGGAGCAGGTGGAAG
  ATCTATGGAACCACAGACAATCTGTGCTCCAGAGGTTATGAAGCCATGTATACGCTACTAGGCAATGCCA
  ATGGAGCAACCTGTGCATTCCCGTTCAAGTTTGAAAACAAGTGGTACGCAGATTGCACGAGTGCTGGGCG
  GTCGGATGGATGGCTCTGGTGCGGAACCACTACTGACTATGACACAGACAAGCTATTTGGATATTGTCCA
  TTGAAATTTGAGGGCAGTGAAAGCTTATGGAATAAAGACCCGCTGACCAGCGTTTCCTACCAGATAAACT
  CCAAATCCGCTTTAACGTGGCACCAAGCGAGGAAAAGCTGCCAACAACAGAACGCTGAGCTCCTGAGCAT
  CACAGAGATACATGAGCAAACATACCTGACAGGATTAACCAGTTCCTTGACCTCAGGACTCTGGATTGGA
  CTTAACAGTCTGAGCTTCAACAGCGGTTGGCAGTGGAGTGACCGCAGTCCTTTCCGATATTTGAACTGGT
  TACCAGGAAGTCCATCAGCTGAACCTGGAAAAAGCTGTGTGTCACTAAATCCTGGAAAAAATGCTAAATG
  GGAAAATCTGGAATGTGTTCAGAAACTGGGCTATATTTGCAAAAAGGGCAACACCACTTTAAATTCTTTT
  GTTATTCCCTCAGAAAGTGATGTGCCTACTCACTGTCCTAGTCAGTGGTGGCCGTATGCCGGTCACTGTT
  ACAAGATTCACAGAGATGAGAAAAAAATCCAGAGGGATGCTCTGACCACCTGCAGGAAGGAAGGCGGTGA
  CCTCACAAGTATCCACACCATCGAGGAATTGGACTTTATTATCTCCCAGCTAGGATATGAGCCAAATGAC
  GAATTGTGGATCGGCTTAAATGACATTAAGATTCAAATGTACTTTGAGTGGAGTGATGGGACCCCTGTAA
  CGTTTACCAAATGGCTTCGTGGAGAACCAAGCCATGAAAACAACAGACAGGAGGATTGTGTGGTGATGAA
  AGGCAAGGATGGGTACTGGGCAGATCGGGGCTGTGAGTGGCCTCTTGGCTACATCTGCAAGATGAAATCA
  CGAAGCCAAGGTCCAGAAATAGTGGAAGTCGAAAAAGGCTGCAGGAAAGGCTGGAAAAAACATCACTTTT
  ACTGCTATATGATTGGACATACGCTTTCAACATTTGCAGAAGCAAACCAAACCTGTAATAATGAGAATGC
  TTATTTAACAACTATTGAAGACAGATATGAACAAGCCTTCCTGACTAGTTTCGTTGGCTTAAGGCCTGAA
  AAATATTTCTGGACAGGACTTTCAGATATACAAACCAAAGGGACTTTTCAGTGGACCATCGAGGAAGAGG
  TTCGGTTCACCCACTGGAATTCAGATATGCCAGGGCGAAAGCCAGGGTGTGTTGCCATGAGAACCGGGAT
  TGCAGGGGGCTTATGGGATGTTTTGAAATGTGATGAAAAGGCAAAATTTGTGTGCAAGCACTGGGCAGAA
  GGAGTAACCCACCCACCGAAGCCCACGACGACTCCCGAACCCAAATGTCCGGAGGATTGGGGCGCCAGCA
  GTAGAACAAGCTTGTGTTTCAAGCTGTATGCAAAAGGAAAACATGAGAAGAAAACGTGGTTTGAATCTCG
  AGATTTTTGTCGAGCTCTGGGTGGAGACTTAGCTAGCATCAATAACAAAGAGGAACAGCAAACAATATGG
  CGATTAATAACAGCTAGTGGAAGCTACCACAAACTGTTTTGGTTGGGATTGACATATGGAAGCCCTTCAG
  AAGGTTTTACTTGGAGTGATGGTTCTCCTGTTTCATATGAAAACTGGGCTTATGGAGAACCTAATAATTA
  TCAAAATGTTGAATACTGTGGTGAGCTGAAAGGTGACCCTACTATGTCTTGGAATGATATTAATTGTGAA
  CACCTTAACAACTGGATTTGCCAGATACAAAAAGGACAAACACCAAAACCTGAGCCAACACCAGCTCCTC
  AAGACAATCCACCAGTTACTGAAGATGGGTGGGTTATTTACAAAGACTACCAGTATTATTTCAGCAAAGA
  GAAGGAAACCATGGACAATGCGCGAGCGTTTTGCAAGAGGAATTTTGGTGATCTTGTTTCTATTCAAAGT
  GAAAGTGAAAAGAAGTTTCTATGGAAATATGTAAACAGAAATGATGCACAGTCTGCATATTTTATTGGTT
  TATTGATCAGCTTGGATAAAAAGTTTGCTTGGATGGATGGAAGCAAAGTGGATTACGTGTCTTGGGCCAC
  AGGTGAACCCAATTTTGCAAATGAAGATGAAAACTGTGTGACCATGTATTCAAATTCAGGGTTTTGGAAT
  GACATTAACTGTGGCTATCCAAACGCCTTCATTTGCCAGCGACATAACAGTAGTATCAATGCTACCACAG
  TTATGCCTACCATGCCCTCGGTCCCATCAGGGTGCAAGGAAGGTTGGAATTTCTACAGCAACAAGTGTTT
  CAAAATCTTTGGATTTATGGAAGAAGAAAGAAAAAATTGGCAAGAGGCACGAAAAGCTTGTATAGGCTTT
  GGAGGGAATCTGGTCTCCATACAAAATGAAAAAGAGCAAGCATTTCTTACCTATCACATGAAGGACTCCA
  CTTTCAGTGCCTGGACTGGGCTGAATGATGTCAATTCAGAACACACGTTCCTTTGGACGGATGGACGAGG
  AGTCCATTACACAAACTGGGGGAAAGGTTACCCTGGTGGAAGAAGAAGCAGTCTTTCTTATGAAGATGCT
  GACTGTGTTGTTATTATTGGAGGTGCATCAAATGAAGCAGGAAAATGGATGGATGATACCTGCGACAGTA
  AACGAGGCTACATATGCCAGACACGATCCGACCCTTCCTTGACTAATCCTCCAGCAACGATTCAAACAGA
  TGGCTTTGTTAAATATGGCAAAAGCAGCTATTCACTCATGAGACAAAAATTTCAATGGCATGAAGCGGAG
  ACATACTGCAAGCTTCACAATTCCCTTATAGCCAGCATTCTGGATCCCTACAGTAATGCATTTGCGTGGC
  TGCAGATGGAAACATCTAATGAACGTGTGTGGATCGCCCTGAACAGTAACTTGACTGATAATCAATACAC
  TTGGACTGATAAGTGGAGGGTGAGGTACACTAACTGGGCTGCTGATGAGCCCAAATTGAAATCAGCATGT
  GTTTATCTGGATCTTGATGGCTACTGGAAGACAGCACATTGCAATGAAAGTTTTTACTTTCTCTGTAAAA
  GATCAGATGAAATCCCTGCTACTGAACCCCCACAACTGCCTGGCAGATGCCCGGAGTCAGATCACACAGC
  ATGGATTCCTTTCCATGGTCACTGTTACTATATTGAGTCCTCATATACAAGAAACTGGGGCCAAGCTTCT
  CTGGAATGTCTTCGAATGGGTTCCTCTCTGGTTTCCATTGAAAGTGCTGCAGAATCCAGTTTTCTGTCAT
  ATCGGGTTGAGCCACTTAAAAGTAAAACCAATTTTTGGATAGGATTGTTCAGAAATGTTGAAGGGACGTG
  GCTGTGGATAAATAACAGTCCGGTCTCCTTTGTCAACTGGAACACAGGAGATCCCTCTGGTGAACGGAAT
  GATTGTGTAGCTTTACATGCGTCTTCTGGGTTTTGGAGTAATATTCACTGTTCTTCCTACAAAGGATATA
  TTTGTAAAAGACCAAAAATTATTGATGCTAAACCTACTCATGAATTACTTACAACAAAAGCTGACACAAG
  GAAGATGGACCCTTCTAAACCGTCTTCCAACGTGGCCGGAGTAGTCATCATTGTGATCCTCCTGATTTTA
  ACGGGTGCTGGCCTTGCCGCCTATTTCTTTTATAAGAAAAGACGTGTGCACCTACCTCAAGAGGGCGCCT
  TTGAAAACACTCTGTATTTTAACAGTCAGTCAAGCCCAGGAACTAGTGATATGAAAGATCTCGTGGGCAA
  TATTGAACAGAATGAACACTCGGTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219343 representing NM_002438
  Red=Cloning site Green=Tags(s)
  
  MRLPLLLVFASVIPGAVLLLDTRQFLIYNEDHKRCVDAVSPSAVQTAACNQDAESQKFRWVSESQIMSVA
  FKLCLGVPSKTDWVAITLYACDSKSEFQKWECKNDTLLGIKGEDLFFNYGNRQEKNIMLYKGSGLWSRWK
  IYGTTDNLCSRGYEAMYTLLGNANGATCAFPFKFENKWYADCTSAGRSDGWLWCGTTTDYDTDKLFGYCP
  LKFEGSESLWNKDPLTSVSYQINSKSALTWHQARKSCQQQNAELLSITEIHEQTYLTGLTSSLTSGLWIG
  LNSLSFNSGWQWSDRSPFRYLNWLPGSPSAEPGKSCVSLNPGKNAKWENLECVQKLGYICKKGNTTLNSF
  VIPSESDVPTHCPSQWWPYAGHCYKIHRDEKKIQRDALTTCRKEGGDLTSIHTIEELDFIISQLGYEPND
  ELWIGLNDIKIQMYFEWSDGTPVTFTKWLRGEPSHENNRQEDCVVMKGKDGYWADRGCEWPLGYICKMKS
  RSQGPEIVEVEKGCRKGWKKHHFYCYMIGHTLSTFAEANQTCNNENAYLTTIEDRYEQAFLTSFVGLRPE
  KYFWTGLSDIQTKGTFQWTIEEEVRFTHWNSDMPGRKPGCVAMRTGIAGGLWDVLKCDEKAKFVCKHWAE
  GVTHPPKPTTTPEPKCPEDWGASSRTSLCFKLYAKGKHEKKTWFESRDFCRALGGDLASINNKEEQQTIW
  RLITASGSYHKLFWLGLTYGSPSEGFTWSDGSPVSYENWAYGEPNNYQNVEYCGELKGDPTMSWNDINCE
  HLNNWICQIQKGQTPKPEPTPAPQDNPPVTEDGWVIYKDYQYYFSKEKETMDNARAFCKRNFGDLVSIQS
  ESEKKFLWKYVNRNDAQSAYFIGLLISLDKKFAWMDGSKVDYVSWATGEPNFANEDENCVTMYSNSGFWN
  DINCGYPNAFICQRHNSSINATTVMPTMPSVPSGCKEGWNFYSNKCFKIFGFMEEERKNWQEARKACIGF
  GGNLVSIQNEKEQAFLTYHMKDSTFSAWTGLNDVNSEHTFLWTDGRGVHYTNWGKGYPGGRRSSLSYEDA
  DCVVIIGGASNEAGKWMDDTCDSKRGYICQTRSDPSLTNPPATIQTDGFVKYGKSSYSLMRQKFQWHEAE
  TYCKLHNSLIASILDPYSNAFAWLQMETSNERVWIALNSNLTDNQYTWTDKWRVRYTNWAADEPKLKSAC
  VYLDLDGYWKTAHCNESFYFLCKRSDEIPATEPPQLPGRCPESDHTAWIPFHGHCYYIESSYTRNWGQAS
  LECLRMGSSLVSIESAAESSFLSYRVEPLKSKTNFWIGLFRNVEGTWLWINNSPVSFVNWNTGDPSGERN
  DCVALHASSGFWSNIHCSSYKGYICKRPKIIDAKPTHELLTTKADTRKMDPSKPSSNVAGVVIIVILLIL
  TGAGLAAYFFYKKRRVHLPQEGAFENTLYFNSQSSPGTSDMKDLVGNIEQNEHSVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002438
• ORF Size: 4368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002438.1, NM_002438.2, NM_002438.3, NP_002429.1
• RefSeq Size: 5205 bp
• RefSeq ORF: 4371 bp
• Locus ID: 4360
• Cytogenetics: 10p12.33
• Protein Families: Druggable Genome, Transmembrane
• MW: 166 kDa
• Gene Summary: 'The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.[provided by RefSeq, Sep 2015]'