Kinesin 5A (KIF5A) (NM_004984) Human Tagged ORF Clone

CAT#: RC219405

KIF5A (Myc-DDK-tagged)-Human kinesin family member 5A (KIF5A)

Product Images

Western blot validation of overexpression lysate (Cat# LY417613) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219405 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIF5A
• Synonyms: ALS25; D12S1889; MY050; NEIMY; NKHC; SPG10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219405 representing NM_004984
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGACCAACAACGAATGTAGCATCAAGGTGCTCTGCCGATTCCGGCCCCTGAACCAGGCTGAGA
  TTCTGCGGGGAGACAAGTTCATCCCCATTTTCCAAGGGGACGACAGCGTCGTTATTGGGGGGAAGCCATA
  TGTTTTTGACCGTGTATTCCCCCCAAACACGACTCAAGAGCAAGTTTATCATGCATGTGCCATGCAGATT
  GTCAAAGATGTCCTTGCTGGCTACAATGGCACCATTTTTGCTTATGGACAGACATCCTCAGGGAAAACAC
  ATACCATGGAGGGAAAGCTGCACGACCCTCAGCTGATGGGAATCATTCCTCGAATTGCCCGAGACATCTT
  CAACCACATCTACTCCATGGATGAGAACCTTGAGTTCCACATCAAGGTTTCTTACTTTGAAATTTACCTG
  GACAAAATTCGTGACCTTCTGGATGTGACCAAGACAAATCTGTCCGTGCACGAGGACAAGAACCGGGTGC
  CATTTGTCAAGGGTTGTACTGAACGCTTTGTGTCCAGCCCGGAGGAGATTCTGGATGTGATTGATGAAGG
  GAAATCAAATCGTCATGTGGCTGTCACCAACATGAATGAACACAGCTCTCGGAGCCACAGCATCTTCCTC
  ATCAACATCAAGCAGGAGAACATGGAAACGGAGCAGAAGCTCAGTGGGAAGCTGTATCTGGTGGACCTGG
  CAGGGAGTGAGAAGGTCAGCAAGACTGGAGCAGAGGGAGCCGTGCTGGACGAGGCAAAGAATATCAACAA
  GTCACTGTCAGCTCTGGGCAATGTGATCTCCGCACTGGCTGAGGGCACTAAAAGCTATGTTCCATATCGT
  GACAGCAAAATGACAAGGATTCTCCAGGACTCTCTCGGGGGAAACTGCCGGACGACTATGTTCATCTGTT
  GCTCACCATCCAGTTATAATGATGCAGAGACCAAGTCCACCCTGATGTTTGGGCAGCGGGCAAAGACCAT
  TAAGAACACTGCCTCAGTAAATTTGGAGTTGACTGCTGAGCAGTGGAAGAAGAAATATGAGAAGGAGAAG
  GAGAAGACAAAGGCCCAGAAGGAGACGATTGCGAAGCTGGAGGCTGAGCTGAGCCGGTGGCGCAATGGAG
  AGAATGTGCCTGAGACAGAGCGCCTGGCTGGGGAGGAGGCAGCCCTGGGAGCCGAGCTCTGTGAGGAGAC
  CCCTGTGAATGACAACTCATCCATCGTGGTGCGCATCGCGCCCGAGGAGCGGCAGAAATACGAGGAGGAG
  ATCCGCCGTCTCTATAAGCAGCTTGACGACAAGGATGATGAAATCAACCAACAAAGCCAACTCATAGAGA
  AGCTCAAGCAGCAAATGCTGGACCAGGAAGAGCTGCTGGTGTCCACCCGAGGAGACAACGAGAAGGTCCA
  GCGGGAGCTGAGCCACCTGCAATCAGAGAACGATGCCGCTAAGGATGAGGTGAAGGAAGTGCTGCAGGCC
  CTGGAGGAGCTGGCTGTGAACTATGACCAGAAGTCCCAGGAGGTGGAGGAGAAGAGCCAGCAGAACCAGC
  TTCTGGTGGATGAGCTGTCTCAGAAGGTGGCCACCATGCTGTCCCTGGAGTCTGAGTTGCAGCGGCTACA
  GGAGGTCAGTGGACACCAGCGAAAACGAATTGCTGAGGTGCTGAACGGGCTGATGAAGGATCTGAGCGAG
  TTCAGTGTCATTGTGGGCAACGGGGAGATTAAGCTGCCAGTGGAGATCAGTGGGGCCATCGAGGAGGAGT
  TCACTGTGGCCCGACTCTACATCAGCAAAATCAAATCAGAAGTCAAGTCTGTGGTCAAGCGGTGCCGGCA
  GCTGGAGAACCTCCAGGTGGAGTGTCACCGCAAGATGGAAGTGACCGGGCGGGAGCTCTCATCCTGCCAG
  CTCCTCATCTCTCAGCATGAGGCCAAGATCCGCTCGCTTACGGAATACATGCAGAGCGTGGAGCTAAAGA
  AGCGGCACCTGGAAGAGTCCTATGACTCCTTGAGCGATGAGCTGGCCAAGCTCCAGGCCCAGGAAACTGT
  GCATGAAGTGGCCCTGAAGGACAAGGAGCCTGACACTCAGGATGCAGATGAAGTGAAGAAGGCTCTGGAG
  CTGCAGATGGAGAGTCACCGGGAGGCCCATCACCGGCAGCTGGCCCGGCTCCGGGACGAGATCAACGAGA
  AGCAGAAGACCATTGATGAGCTCAAAGACCTAAATCAGAAGCTCCAGTTAGAGCTAGAGAAGCTTCAGGC
  TGACTACGAGAAGCTGAAGAGCGAAGAACACGAGAAGAGCACCAAGCTGCAGGAGCTGACATTTCTGTAC
  GAGCGACATGAGCAGTCCAAGCAGGACCTCAAGGGTCTGGAGGAGACAGTTGCCCGGGAACTCCAGACCC
  TCCACAACCTTCGCAAGCTGTTCGTTCAAGACGTCACGACTCGAGTCAAGAAAAGTGCAGAAATGGAGCC
  CGAAGACAGTGGGGGGATTCACTCCCAAAAGCAGAAGATTTCCTTTCTTGAGAACAACCTGGAACAGCTT
  ACAAAGGTTCACAAACAGCTGGTACGTGACAATGCAGATCTGCGTTGTGAGCTTCCTAAATTGGAAAAAC
  GACTTAGGGCTACGGCTGAGAGAGTTAAGGCCCTGGAGGGTGCACTGAAGGAGGCCAAGGAGGGCGCCAT
  GAAGGACAAGCGCCGGTACCAGCAGGAGGTGGACCGCATCAAGGAGGCCGTTCGCTACAAGAGCTCGGGC
  AAACGGGGCCATTCTGCCCAGATTGCCAAACCCGTCCGGCCTGGCCACTACCCAGCATCCTCACCCACCA
  ACCCCTATGGCACCCGGAGCCCTGAGTGCATCAGTTACACCAACAGCCTCTTCCAGAACTACCAGAATCT
  CTACCTGCAGGCCACACCCAGCTCCACCTCAGATATGTACTTTGCAAACTCCTGTACCAGCAGTGGAGCC
  ACATCTTCTGGCGGCCCCTTGGCTTCCTACCAGAAGGCCAACATGGACAATGGAAATGCCACAGATATCA
  ATGACAATAGGAGTGACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTTTCCCTCTCCACCA
  AGAGACAGCAGCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219405 representing NM_004984
  Red=Cloning site Green=Tags(s)
  
  MAETNNECSIKVLCRFRPLNQAEILRGDKFIPIFQGDDSVVIGGKPYVFDRVFPPNTTQEQVYHACAMQI
  VKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYSMDENLEFHIKVSYFEIYL
  DKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFL
  INIKQENMETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYR
  DSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEK
  EKTKAQKETIAKLEAELSRWRNGENVPETERLAGEEAALGAELCEETPVNDNSSIVVRIAPEERQKYEEE
  IRRLYKQLDDKDDEINQQSQLIEKLKQQMLDQEELLVSTRGDNEKVQRELSHLQSENDAAKDEVKEVLQA
  LEELAVNYDQKSQEVEEKSQQNQLLVDELSQKVATMLSLESELQRLQEVSGHQRKRIAEVLNGLMKDLSE
  FSVIVGNGEIKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQ
  LLISQHEAKIRSLTEYMQSVELKKRHLEESYDSLSDELAKLQAQETVHEVALKDKEPDTQDADEVKKALE
  LQMESHREAHHRQLARLRDEINEKQKTIDELKDLNQKLQLELEKLQADYEKLKSEEHEKSTKLQELTFLY
  ERHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTTRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQL
  TKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKSSG
  KRGHSAQIAKPVRPGHYPASSPTNPYGTRSPECISYTNSLFQNYQNLYLQATPSSTSDMYFANSCTSSGA
  TSSGGPLASYQKANMDNGNATDINDNRSDLPCGYEAEDQAKLFPLHQETAAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004984
• ORF Size: 3096 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004984.1, NM_004984.2, NP_004975.2
• RefSeq Size: 3897 bp
• RefSeq ORF: 3099 bp
• Locus ID: 3798
• Cytogenetics: 12q13.3
• Domains: kinesin
• Protein Families: Druggable Genome
• MW: 117.4 kDa
• Gene Summary: 'This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq, Jul 2008]'