Kinesin 5A (KIF5A) (NM_004984) Human Tagged ORF Clone
CAT#: RC219405
KIF5A (Myc-DDK-tagged)-Human kinesin family member 5A (KIF5A)
"NM_004984" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | KIF5A |
Synonyms | ALS25; D12S1889; MY050; NEIMY; NKHC; SPG10 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219405 representing NM_004984
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGACCAACAACGAATGTAGCATCAAGGTGCTCTGCCGATTCCGGCCCCTGAACCAGGCTGAGA TTCTGCGGGGAGACAAGTTCATCCCCATTTTCCAAGGGGACGACAGCGTCGTTATTGGGGGGAAGCCATA TGTTTTTGACCGTGTATTCCCCCCAAACACGACTCAAGAGCAAGTTTATCATGCATGTGCCATGCAGATT GTCAAAGATGTCCTTGCTGGCTACAATGGCACCATTTTTGCTTATGGACAGACATCCTCAGGGAAAACAC ATACCATGGAGGGAAAGCTGCACGACCCTCAGCTGATGGGAATCATTCCTCGAATTGCCCGAGACATCTT CAACCACATCTACTCCATGGATGAGAACCTTGAGTTCCACATCAAGGTTTCTTACTTTGAAATTTACCTG GACAAAATTCGTGACCTTCTGGATGTGACCAAGACAAATCTGTCCGTGCACGAGGACAAGAACCGGGTGC CATTTGTCAAGGGTTGTACTGAACGCTTTGTGTCCAGCCCGGAGGAGATTCTGGATGTGATTGATGAAGG GAAATCAAATCGTCATGTGGCTGTCACCAACATGAATGAACACAGCTCTCGGAGCCACAGCATCTTCCTC ATCAACATCAAGCAGGAGAACATGGAAACGGAGCAGAAGCTCAGTGGGAAGCTGTATCTGGTGGACCTGG CAGGGAGTGAGAAGGTCAGCAAGACTGGAGCAGAGGGAGCCGTGCTGGACGAGGCAAAGAATATCAACAA GTCACTGTCAGCTCTGGGCAATGTGATCTCCGCACTGGCTGAGGGCACTAAAAGCTATGTTCCATATCGT GACAGCAAAATGACAAGGATTCTCCAGGACTCTCTCGGGGGAAACTGCCGGACGACTATGTTCATCTGTT GCTCACCATCCAGTTATAATGATGCAGAGACCAAGTCCACCCTGATGTTTGGGCAGCGGGCAAAGACCAT TAAGAACACTGCCTCAGTAAATTTGGAGTTGACTGCTGAGCAGTGGAAGAAGAAATATGAGAAGGAGAAG GAGAAGACAAAGGCCCAGAAGGAGACGATTGCGAAGCTGGAGGCTGAGCTGAGCCGGTGGCGCAATGGAG AGAATGTGCCTGAGACAGAGCGCCTGGCTGGGGAGGAGGCAGCCCTGGGAGCCGAGCTCTGTGAGGAGAC CCCTGTGAATGACAACTCATCCATCGTGGTGCGCATCGCGCCCGAGGAGCGGCAGAAATACGAGGAGGAG ATCCGCCGTCTCTATAAGCAGCTTGACGACAAGGATGATGAAATCAACCAACAAAGCCAACTCATAGAGA AGCTCAAGCAGCAAATGCTGGACCAGGAAGAGCTGCTGGTGTCCACCCGAGGAGACAACGAGAAGGTCCA GCGGGAGCTGAGCCACCTGCAATCAGAGAACGATGCCGCTAAGGATGAGGTGAAGGAAGTGCTGCAGGCC CTGGAGGAGCTGGCTGTGAACTATGACCAGAAGTCCCAGGAGGTGGAGGAGAAGAGCCAGCAGAACCAGC TTCTGGTGGATGAGCTGTCTCAGAAGGTGGCCACCATGCTGTCCCTGGAGTCTGAGTTGCAGCGGCTACA GGAGGTCAGTGGACACCAGCGAAAACGAATTGCTGAGGTGCTGAACGGGCTGATGAAGGATCTGAGCGAG TTCAGTGTCATTGTGGGCAACGGGGAGATTAAGCTGCCAGTGGAGATCAGTGGGGCCATCGAGGAGGAGT TCACTGTGGCCCGACTCTACATCAGCAAAATCAAATCAGAAGTCAAGTCTGTGGTCAAGCGGTGCCGGCA GCTGGAGAACCTCCAGGTGGAGTGTCACCGCAAGATGGAAGTGACCGGGCGGGAGCTCTCATCCTGCCAG CTCCTCATCTCTCAGCATGAGGCCAAGATCCGCTCGCTTACGGAATACATGCAGAGCGTGGAGCTAAAGA AGCGGCACCTGGAAGAGTCCTATGACTCCTTGAGCGATGAGCTGGCCAAGCTCCAGGCCCAGGAAACTGT GCATGAAGTGGCCCTGAAGGACAAGGAGCCTGACACTCAGGATGCAGATGAAGTGAAGAAGGCTCTGGAG CTGCAGATGGAGAGTCACCGGGAGGCCCATCACCGGCAGCTGGCCCGGCTCCGGGACGAGATCAACGAGA AGCAGAAGACCATTGATGAGCTCAAAGACCTAAATCAGAAGCTCCAGTTAGAGCTAGAGAAGCTTCAGGC TGACTACGAGAAGCTGAAGAGCGAAGAACACGAGAAGAGCACCAAGCTGCAGGAGCTGACATTTCTGTAC GAGCGACATGAGCAGTCCAAGCAGGACCTCAAGGGTCTGGAGGAGACAGTTGCCCGGGAACTCCAGACCC TCCACAACCTTCGCAAGCTGTTCGTTCAAGACGTCACGACTCGAGTCAAGAAAAGTGCAGAAATGGAGCC CGAAGACAGTGGGGGGATTCACTCCCAAAAGCAGAAGATTTCCTTTCTTGAGAACAACCTGGAACAGCTT ACAAAGGTTCACAAACAGCTGGTACGTGACAATGCAGATCTGCGTTGTGAGCTTCCTAAATTGGAAAAAC GACTTAGGGCTACGGCTGAGAGAGTTAAGGCCCTGGAGGGTGCACTGAAGGAGGCCAAGGAGGGCGCCAT GAAGGACAAGCGCCGGTACCAGCAGGAGGTGGACCGCATCAAGGAGGCCGTTCGCTACAAGAGCTCGGGC AAACGGGGCCATTCTGCCCAGATTGCCAAACCCGTCCGGCCTGGCCACTACCCAGCATCCTCACCCACCA ACCCCTATGGCACCCGGAGCCCTGAGTGCATCAGTTACACCAACAGCCTCTTCCAGAACTACCAGAATCT CTACCTGCAGGCCACACCCAGCTCCACCTCAGATATGTACTTTGCAAACTCCTGTACCAGCAGTGGAGCC ACATCTTCTGGCGGCCCCTTGGCTTCCTACCAGAAGGCCAACATGGACAATGGAAATGCCACAGATATCA ATGACAATAGGAGTGACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTTTCCCTCTCCACCA AGAGACAGCAGCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219405 representing NM_004984
Red=Cloning site Green=Tags(s) MAETNNECSIKVLCRFRPLNQAEILRGDKFIPIFQGDDSVVIGGKPYVFDRVFPPNTTQEQVYHACAMQI VKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYSMDENLEFHIKVSYFEIYL DKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFL INIKQENMETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYR DSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEK EKTKAQKETIAKLEAELSRWRNGENVPETERLAGEEAALGAELCEETPVNDNSSIVVRIAPEERQKYEEE IRRLYKQLDDKDDEINQQSQLIEKLKQQMLDQEELLVSTRGDNEKVQRELSHLQSENDAAKDEVKEVLQA LEELAVNYDQKSQEVEEKSQQNQLLVDELSQKVATMLSLESELQRLQEVSGHQRKRIAEVLNGLMKDLSE FSVIVGNGEIKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQ LLISQHEAKIRSLTEYMQSVELKKRHLEESYDSLSDELAKLQAQETVHEVALKDKEPDTQDADEVKKALE LQMESHREAHHRQLARLRDEINEKQKTIDELKDLNQKLQLELEKLQADYEKLKSEEHEKSTKLQELTFLY ERHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTTRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQL TKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKSSG KRGHSAQIAKPVRPGHYPASSPTNPYGTRSPECISYTNSLFQNYQNLYLQATPSSTSDMYFANSCTSSGA TSSGGPLASYQKANMDNGNATDINDNRSDLPCGYEAEDQAKLFPLHQETAAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_004984 |
ORF Size | 3096 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004984.1, NM_004984.2, NP_004975.2 |
RefSeq Size | 3897 bp |
RefSeq ORF | 3099 bp |
Locus ID | 3798 |
Cytogenetics | 12q13.3 |
Domains | kinesin |
Protein Families | Druggable Genome |
MW | 117.4 kDa |
Gene Summary | 'This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC311122 | KIF5A (untagged)-Human kinesin family member 5A (KIF5A) |
USD 1,100.00 |
|
RG219405 | KIF5A (GFP-tagged) - Human kinesin family member 5A (KIF5A) |
USD 970.00 |
|
RC219405L3 | Lenti-ORF clone of KIF5A (Myc-DDK-tagged)-Human kinesin family member 5A (KIF5A) |
USD 1,080.00 |
|
RC219405L4 | Lenti-ORF clone of KIF5A (mGFP-tagged)-Human kinesin family member 5A (KIF5A) |
USD 1,080.00 |
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