Kinesin 5A (KIF5A) (NM_004984) Human Untagged Clone

CAT#: SC311122

KIF5A (untagged)-Human kinesin family member 5A (KIF5A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KIF5A
• Synonyms: ALS25; D12S1889; MY050; NEIMY; NKHC; SPG10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004984, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGAGACCAACAACGAATGTAGCATCAAGGTGCTCTGCCGATTCCGGCCCCTGAACCAGGCTGAGA
  TTCTGCGGGGAGACAAGTTCATCCCCATTTTCCAAGGGGACGACAGCGTCGTTATTGGGGGGAAGCCATA
  TGTTTTTGACCGTGTATTCCCCCCAAACACGACTCAAGAGCAAGTTTATCATGCATGTGCCATGCAGATT
  GTCAAAGATGTCCTTGCTGGCTACAATGGCACCATTTTTGCTTATGGACAGACATCCTCAGGGAAAACAC
  ATACCATGGAGGGAAAGCTGCACGACCCTCAGCTGATGGGAATCATTCCTCGAATTGCCCGAGACATCTT
  CAACCACATCTACTCCATGGATGAGAACCTTGAGTTCCACATCAAGGTTTCTTACTTTGAAATTTACCTG
  GACAAAATTCGTGACCTTCTGGATGTGACCAAGACAAATCTGTCCGTGCACGAGGACAAGAACCGGGTGC
  CATTTGTCAAGGGTTGTACTGAACGCTTTGTGTCCAGCCCGGAGGAGATTCTGGATGTGATTGATGAAGG
  GAAATCAAATCGTCATGTGGCTGTCACCAACATGAATGAACACAGCTCTCGGAGCCACAGCATCTTCCTC
  ATCAACATCAAGCAGGAGAACATGGAAACGGAGCAGAAGCTCAGTGGGAAGCTGTATCTGGTGGACCTGG
  CAGGGAGTGAGAAGGTCAGCAAGACTGGAGCAGAGGGAGCCGTGCTGGACGAGGCAAAGAATATCAACAA
  GTCACTGTCAGCTCTGGGCAATGTGATCTCCGCACTGGCTGAGGGCACTAAAAGCTATGTTCCATATCGT
  GACAGCAAAATGACAAGGATTCTCCAGGACTCTCTCGGGGGAAACTGCCGGACGACTATGTTCATCTGTT
  GCTCACCATCCAGTTATAATGATGCAGAGACCAAGTCCACCCTGATGTTTGGGCAGCGGGCAAAGACCAT
  TAAGAACACTGCCTCAGTAAATTTGGAGTTGACTGCTGAGCAGTGGAAGAAGAAATATGAGAAGGAGAAG
  GAGAAGACAAAGGCCCAGAAGGAGACGATTGCGAAGCTGGAGGCTGAGCTGAGCCGGTGGCGCAATGGAG
  AGAATGTGCCTGAGACAGAGCGCCTGGCTGGGGAGGAGGCAGCCCTGGGAGCCGAGCTCTGTGAGGAGAC
  CCCTGTGAATGACAACTCATCCATCGTGGTGCGCATCGCGCCCGAGGAGCGGCAGAAATACGAGGAGGAG
  ATCCGCCGTCTCTATAAGCAGCTTGACGACAAGGATGATGAAATCAACCAACAAAGCCAACTCATAGAGA
  AGCTCAAGCAGCAAATGCTGGACCAGGAAGAGCTGCTGGTGTCCACCCGAGGAGACAACGAGAAGGTCCA
  GCGGGAGCTGAGCCACCTGCAATCAGAGAACGATGCCGCTAAGGATGAGGTGAAGGAAGTGCTGCAGGCC
  CTGGAGGAGCTGGCTGTGAACTATGACCAGAAGTCCCAGGAGGTGGAGGAGAAGAGCCAGCAGAACCAGC
  TTCTGGTGGATGAGCTGTCTCAGAAGGTGGCCACCATGCTGTCCCTGGAGTCTGAGTTGCAGCGGCTACA
  GGAGGTCAGTGGACACCAGCGAAAACGAATTGCTGAGGTGCTGAACGGGCTGATGAAGGATCTGAGCGAG
  TTCAGTGTCATTGTGGGCAACGGGGAGATTAAGCTGCCAGTGGAGATCAGTGGGGCCATCGAGGAGGAGT
  TCACTGTGGCCCGACTCTACATCAGCAAAATCAAATCAGAAGTCAAGTCTGTGGTCAAGCGGTGCCGGCA
  GCTGGAGAACCTCCAGGTGGAGTGTCACCGCAAGATGGAAGTGACCGGGCGGGAGCTCTCATCCTGCCAG
  CTCCTCATCTCTCAGCATGAGGCCAAGATCCGCTCGCTTACGGAATACATGCAGAGCGTGGAGCTAAAGA
  AGCGGCACCTGGAAGAGTCCTATGACTCCTTGAGCGATGAGCTGGCCAAGCTCCAGGCCCAGGAAACTGT
  GCATGAAGTGGCCCTGAAGGACAAGGAGCCTGACACTCAGGATGCAGATGAAGTGAAGAAGGCTCTGGAG
  CTGCAGATGGAGAGTCACCGGGAGGCCCATCACCGGCAGCTGGCCCGGCTCCGGGACGAGATCAACGAGA
  AGCAGAAGACCATTGATGAGCTCAAAGACCTAAATCAGAAGCTCCAGTTAGAGCTAGAGAAGCTTCAGGC
  TGACTACGAGAAGCTGAAGAGCGAAGAACACGAGAAGAGCACCAAGCTGCAGGAGCTGACATTTCTGTAC
  GAGCGACATGAGCAGTCCAAGCAGGACCTCAAGGGTCTGGAGGAGACAGTTGCCCGGGAACTCCAGACCC
  TCCACAACCTTCGCAAGCTGTTCGTTCAAGACGTCACGACTCGAGTCAAGAAAAGTGCAGAAATGGAGCC
  CGAAGACAGTGGGGGGATTCACTCCCAAAAGCAGAAGATTTCCTTTCTTGAGAACAACCTGGAACAGCTT
  ACAAAGGTTCACAAACAGCTGGTACGTGACAATGCAGATCTGCGTTGTGAGCTTCCTAAATTGGAAAAAC
  GACTTAGGGCTACGGCTGAGAGAGTTAAGGCCCTGGAGGGTGCACTGAAGGAGGCCAAGGAGGGCGCCAT
  GAAGGACAAGCGCCGGTACCAGCAGGAGGTGGACCGCATCAAGGAGGCCGTTCGCTACAAGAGCTCGGGC
  AAACGGGGCCATTCTGCCCAGATTGCCAAACCCGTCCGGCCTGGCCACTACCCAGCATCCTCACCCACCA
  ACCCCTATGGCACCCGGAGCCCTGAGTGCATCAGTTACACCAACAGCCTCTTCCAGAACTACCAGAATCT
  CTACCTGCAGGCCACACCCAGCTCCACCTCAGATATGTACTTTGCAAACTCCTGTACCAGCAGTGGAGCC
  ACATCTTCTGGCGGCCCCTTGGCTTCCTACCAGAAGGCCAACATGGACAATGGAAATGCCACAGATATCA
  ATGACAATAGGAGTGACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTTTCCCTCTCCACCA
  AGAGACAGCAGCCAGCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004984
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004984.2, NP_004975.2
• RefSeq Size: 3897 bp
• RefSeq ORF: 3099 bp
• Locus ID: 3798
• Cytogenetics: 12q13.3
• Domains: kinesin
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq, Jul 2008]'