LARP2 (LARP1B) (NM_032239) Human Tagged ORF Clone

CAT#: RC219586

LARP1B (Myc-DDK-tagged)-Human La ribonucleoprotein domain family, member 1B (LARP1B), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY410262) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219586 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LARP1B
• Synonyms: LARP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219586 representing NM_032239
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATTGGCCAACACCAAGTGAATTAGTGAACACTGGATTTCAGAGCGTCCTCAGCCAAGGAAATA
  AAAAGCCACAAAATAGAAAAGAAAAAGAAGAGAAGGTTGAAAAGAGAAGTAACAGTGACAGCAAAGAAAA
  CCGGGAAACAAAATTAAATGGTCCTGGTGAAAACGTCAGTGAGGATGAGGCTCAGTCAAGTAATCAACGT
  AAGAGAGCTAATAAGCACAAGTGGGTACCACTCCACTTAGATGTTGTAAGATCAGAGAGTCAAGAAAGAC
  CTGGATCCCGGAACAGCTCAAGATGTCAACCTGAAGCAAATAAACCAACACATAACAATAGGAGAAATGA
  TACACGAAGTTGGAAGCGAGATAGAGAAAAAAGAGATGATCAAGATGACGTTTCCAGTGTGAGAAGTGAG
  GGTGGTAATATCCGAGGTTCCTTTAGAGGTCGAGGAAGAGGCCGAGGACGGGGAAGAGGACGAGGCAGAG
  GAAATCCTCGATTGAACTTTGATTATTCATATGGTTATCAAGAACATGGTGAAAGGACTGATCAACCATT
  TCAAACAGAACTTAATACCAGTATGATGTATTACTATGATGATGGTACAGGTGTACAGGTGTATCCTGTG
  GAAGAAGCGTTGCTTAAAGAGTATATTAAGCGTCAAATTGAATATTACTTCAGTGTAGAAAATTTGGAAC
  GAGACTTCTTTCTTCGGGGAAAGATGGATGAACAAGGTTTCTTGCCTATTTCCCTGATTGCTGGTTTTCA
  GCGTGTTCAGGCTCTCACTACAAACCTTAATCTCATCTTAGAGGCACTGAAGGATAGCACAGAAGTAGAA
  ATTGTGGATGAGAAAATGAGAAAAAAGATAGAACCAGAAAAATGGCCAATTCCAGGCCCTCCTCCACGCA
  GTGTGCCACCAACAGACTTCTCTCAACTGATTGATTGTCCAGAGTTTGTACCAGGCCAAGCCTTTTGCTC
  ACATACAGGTAACATCTTTTCTTCTAGAGCAAACGATGTAACAGTGGGTTATTTGGTCCAATTTACTTAC
  TTATTTATTTATTTTCAGTTTATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219586 representing NM_032239
  Red=Cloning site Green=Tags(s)
  
  MENWPTPSELVNTGFQSVLSQGNKKPQNRKEKEEKVEKRSNSDSKENRETKLNGPGENVSEDEAQSSNQR
  KRANKHKWVPLHLDVVRSESQERPGSRNSSRCQPEANKPTHNNRRNDTRSWKRDREKRDDQDDVSSVRSE
  GGNIRGSFRGRGRGRGRGRGRGRGNPRLNFDYSYGYQEHGERTDQPFQTELNTSMMYYYDDGTGVQVYPV
  EEALLKEYIKRQIEYYFSVENLERDFFLRGKMDEQGFLPISLIAGFQRVQALTTNLNLILEALKDSTEVE
  IVDEKMRKKIEPEKWPIPGPPPRSVPPTDFSQLIDCPEFVPGQAFCSHTGNIFSSRANDVTVGYLVQFTY
  LFIYFQFI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032239
• ORF Size: 1074 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032239.2, NM_032239.3, NP_115615.2
• RefSeq Size: 2718 bp
• RefSeq ORF: 1077 bp
• Locus ID: 55132
• Domains: DM15
• MW: 41.2 kDa
• Gene Summary: This gene encodes a protein containing domains found in the La related protein of Drosophila melanogaster. La motif-containing proteins are thought to be RNA-binding proteins, where the La motif and adjacent amino acids fold into an RNA recognition motif. The La motif is also found in proteins unrelated to the La protein. Alternative splicing has been observed at this locus and multiple variants, encoding distinct isoforms, are described. Additional splice variation has been identified but the full-length nature of these transcripts has not been determined. [provided by RefSeq, Jun 2013]