TRPV5 (NM_019841) Human Tagged ORF Clone

CAT#: RC219654

TRPV5 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 5 (TRPV5)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPV5
• Synonyms: CAT2; ECAC1; OTRPC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219654 representing NM_019841
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGGTTTTCTACCTAAGGCAGAAGGGCCCGGGAGCCAACTCCAGAAACTTCTGCCCTCCTTTCTGG
  TCAGAGAACAAGACTGGGACCAGCACCTGGACAAGCTTCATATGCTGCAGCAGAAGAGGATTCTAGAGTC
  TCCACTGCTTCGAGCATCCAAGGAAAATGACCTGTCTGTTCTTAGGCAACTTCTACTGGACTGCACCTGT
  GACGTTCGACAAAGAGGAGCCCTGGGGGAGACGGCGCTGCACATAGCAGCCCTCTATGACAACTTGGAGG
  CGGCCTTGGTGCTGATGGAGGCTGCCCCAGAGCTGGTCTTTGAGCCCACCACATGTGAGGCTTTTGCAGG
  TCAGACTGCACTGCACATCGCTGTTGTGAACCAGAATGTGAACCTGGTGCGTGCCCTGCTCACCCGCAGG
  GCCAGTGTCTCTGCCAGAGCCACAGGCACTGCCTTCCGCCGTAGTCCCCGCAACCTCATCTACTTTGGGG
  AGCACCCTTTGTCCTTTGCTGCCTGTGTGAACAGCGAGGAGATCGTGCGGCTGCTCATTGAGCATGGAGC
  TGACATCAGGGCCCAGGACTCCCTGGGAAACACAGTATTACACATCCTCATCCTCCAGCCCAACAAAACC
  TTTGCCTGCCAGATGTACAACCTGCTGCTGTCCTACGATGGACATGGGGACCACCTGCAGCCCCTGGACC
  TTGTGCCCAATCACCAGGGTCTCACCCCCTTCAAGCTGGCTGGAGTGGAGGGTAACACTGTGATGTTCCA
  GCACCTGATGCAGAAGCGGAGGCACATCCAGTGGACGTATGGACCCCTGACCTCCATTCTCTACGACCTC
  ACAGAGATCGACTCCTGGGGAGAGGAGCTGTCCTTCCTGGAGCTTGTGGTCTCCTCTGATAAACGAGAGG
  CTCGCCAAATTCTGGAACAGACCCCAGTGAAGGAGCTGGTGAGCTTCAAGTGGAACAAGTATGGCCGGCC
  GTACTTCTGCATCCTGGCTGCCTTGTACCTGCTCTACATGATCTGCTTTACCACGTGCTGCGTCTACCGC
  CCCCTTAAGTTTCGTGGTGGCAACCGCACTCATTCTCGAGACATCACCATCCTCCAGCAAAAACTACTAC
  AGGAGGCCTATGAGACACGTGAAGATATCATCAGGCTGGTGGGGGAGCTGGTGAGCATCGTTGGGGCTGT
  GATCATCCTGCTCCTAGAGATTCCAGACATCTTCAGGGTTGGTGCCTCTCGCTATTTTGGAAAGACGATT
  CTTGGGGGGCCATTCCATGTCATCATCATCACCTATGCCTCCCTGGTGCTGGTGACCATGGTGATGCGGC
  TCACCAACACCAATGGGGAGGTGGTGCCCATGTCCTTTGCCCTGGTGCTGGGCTGGTGCAGTGTCATGTA
  TTTCACTCGAGGATTCCAGATGCTGGGTCCCTTCACCATCATGATCCAGAAGATGATTTTTGGAGACCTA
  ATGCGTTTCTGCTGGCTGATGGCTGTGGTCATCTTGGGATTTGCCTCCGCGTTCTATATCATTTTCCAGA
  CAGAGGACCCAACCAGTCTGGGGCAATTCTATGACTACCCCATGGCACTGTTCACCACCTTTGAGCTTTT
  TCTCACTGTTATTGATGCACCTGCCAACTACGACGTGGACTTGCCCTTCATGTTCAGCATTGTCAACTTC
  GCCTTCGCCATCATTGCCACACTGCTCATGCTCAACTTGTTCATCGCCATGATGGGCGACACCCACTGGA
  GGGTGGCCCAGGAGAGGGATGAGCTCTGGAGGGCCCAGGTCGTGGCCACCACAGTGATGCTGGAGCGGAA
  GCTGCCTCGCTGCCTGTGGCCTCGCTCCGGGATCTGTGGGTGCGAATTCGGGCTGGGGGACCGCTGGTTC
  CTGCGGGTTGAGAACCACAATGATCAGAATCCTCTGCGAGTGCTTCGCTATGTGGAAGTGTTCAAGAACT
  CAGACAAGGAGGATGACCAGGAGCATCCATCTGAGAAACAGCCCTCTGGGGCTGAGAGTGGGACTCTAGC
  CAGAGCCTCTTTGGCTCTTCCAACTTCCTCCCTGTCCCGGACCGCGTCCCAGAGCAGCAGTCACCGAGGC
  TGGGAGATCCTTCGTCAAAACACCCTGGGGCACTTGAATCTTGGACTGAACCTTAGTGAGGGGGATGGAG
  AGGAGGTCTACCATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219654 representing NM_019841
  Red=Cloning site Green=Tags(s)
  
  MGGFLPKAEGPGSQLQKLLPSFLVREQDWDQHLDKLHMLQQKRILESPLLRASKENDLSVLRQLLLDCTC
  DVRQRGALGETALHIAALYDNLEAALVLMEAAPELVFEPTTCEAFAGQTALHIAVVNQNVNLVRALLTRR
  ASVSARATGTAFRRSPRNLIYFGEHPLSFAACVNSEEIVRLLIEHGADIRAQDSLGNTVLHILILQPNKT
  FACQMYNLLLSYDGHGDHLQPLDLVPNHQGLTPFKLAGVEGNTVMFQHLMQKRRHIQWTYGPLTSILYDL
  TEIDSWGEELSFLELVVSSDKREARQILEQTPVKELVSFKWNKYGRPYFCILAALYLLYMICFTTCCVYR
  PLKFRGGNRTHSRDITILQQKLLQEAYETREDIIRLVGELVSIVGAVIILLLEIPDIFRVGASRYFGKTI
  LGGPFHVIIITYASLVLVTMVMRLTNTNGEVVPMSFALVLGWCSVMYFTRGFQMLGPFTIMIQKMIFGDL
  MRFCWLMAVVILGFASAFYIIFQTEDPTSLGQFYDYPMALFTTFELFLTVIDAPANYDVDLPFMFSIVNF
  AFAIIATLLMLNLFIAMMGDTHWRVAQERDELWRAQVVATTVMLERKLPRCLWPRSGICGCEFGLGDRWF
  LRVENHNDQNPLRVLRYVEVFKNSDKEDDQEHPSEKQPSGAESGTLARASLALPTSSLSRTASQSSSHRG
  WEILRQNTLGHLNLGLNLSEGDGEEVYHF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019841
• ORF Size: 2187 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019841.2, NM_019841.3, NM_019841.4, NM_019841.5, NM_019841.6, NP_062815.2
• RefSeq Size: 2486
• RefSeq ORF: 2190
• Locus ID: 56302
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• MW: 83 kDa
• Gene Summary: This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. [provided by RefSeq, Jul 2008]