Prickle 2 (PRICKLE2) (NM_198859) Human Tagged ORF Clone

CAT#: RC219763

PRICKLE2 (Myc-DDK-tagged)-Human prickle homolog 2 (Drosophila) (PRICKLE2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRICKLE2
• Synonyms: EPM5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219763 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACAGTGATGCCGCTGGAGATGGAGAAGACCATCAGCAAACTCATGTTTGACTTTCAGAGGAACT
  CGACCTCAGATGATGACTCAGGCTGTGCTTTGGAAGAGTATGCCTGGGTCCCGCCGGGTCTGAAGCCTGA
  ACAGGTACACCAGTACTATAGCTGTCTCCCAGAAGAGAAAGTCCCTTATGTCAACAGTCCTGGAGAGAAA
  CTGCGAATCAAGCAGCTACTACACCAGCTGCCGCCACATGACAATGAGGTTCGATATTGCAACTCCCTGG
  ATGAGGAAGAGAAGAGGGAGCTGAAGCTTTTCAGCAGCCAGAGGAAACGCGAAAACTTGGGCCGCGGGAA
  TGTCAGGCCTTTCCCAGTCACCATGACAGGAGCTATTTGTGAACAGTGCGGAGGCCAGATCAATGGTGGA
  GACATCGCTGTGTTTGCGTCACGCGCTGGCCACGGCGTTTGCTGGCACCCGCCGTGCTTCGTATGCACTG
  TCTGCAATGAGCTCCTGGTGGATCTGATCTACTTTTACCAAGATGGGAAGATATACTGTGGCAGGCACCA
  TGCTGAGTGCCTGAAGCCGCGCTGTGCTGCCTGCGATGAGATCATCTTTGCAGATGAATGCACAGAAGCT
  GAGGGGCGACACTGGCACATGAAACACTTTTGCTGCTTCGAGTGTGAGACAGTGCTGGGCGGCCAGCGCT
  ACATCATGAAGGAGGGAAGACCCTACTGTTGCCACTGCTTCGAGTCCTTGTATGCAGAATATTGTGACAC
  CTGTGCCCAACATATAGGTATCGACCAAGGTCAAATGACCTATGACGGCCAACACTGGCATGCCACTGAG
  ACCTGTTTCTGCTGTGCTCACTGCAAGAAATCCCTCCTGGGGCGGCCATTCCTCCCGAAGCAGGGCCAGA
  TATTCTGCTCACGGGCCTGCAGTGCTGGGGAAGACCCCAATGGTTCTGACTCCTCTGATTCCGCCTTCCA
  GAACGCCAGGGCCAAGGAGTCCCGGCGCAGTGCCAAAATTGGCAAGAACAAGGGCAAGACGGAGGAGCCC
  ATGCTGAACCAGCACAGCCAGCTGCAAGTGAGTTCTAACCGGCTGTCAGCCGACGTAGACCCCCTGTCAC
  TGCAGATGGACATGCTCAGCCTGTCCAGCCAGACACCCAGCCTCAACCGGGACCCCATCTGGAGGAGCCG
  GGAAGAGCCCTACCATTATGGGAACAAGATGGAGCAGAACCAGACCCAGAGCCCTCTGCAGCTCCTCAGC
  CAGTGCAACATCAGAACTTCCTACAGTCCAGGAGGGCAAGGGGCTGGGGCCCAGCCCGAAATGTGGGGCA
  AGCACTTCAGCAACCCCAAAAGGAGCTCGTCACTGGCCATGACAGGACATGCTGGCAGCTTCATCAAGGA
  ATGCCGAGAAGACTATTACCCGGGGAGGCTGAGATCTCAGGAGAGCTACAGTGATATGTCTAGTCAGAGT
  TTCAGTGAGACCCGAGGCAGCATCCAAGTCCCCAAATATGAGGAGGAAGAGGAAGAGGAAGGGGGCTTGT
  CCACTCAGCAGTGTCGGACCCGTCATCCCATCAGTTCCCTGAAATACACAGAGGACATGACGCCCACAGA
  GCAGACCCCTCGGGGCTCCATGGAATCCCTGGCCCTGTCTAATGCAACAGGCCTCTCTGCTGATGGTGGT
  GCCAAGCGCCAGGAGCACCTATCCCGATTTTCCATGCCTGACCTCAGCAAAGACTCTGGAATGAATGTGT
  CTGAGAAGCTGAGCAACATGGGCACTCTTAACTCGTCCATGCAGTTCCGGAGCGCAGAGTCAGTTCGCAG
  CCTGCTCTCTGCCCAGCAGTACCAGGAGATGGAGGGAAACCTCCACCAGCTCAGCAACCCCATTGGCTAC
  AGAGACCTGCAGTCCCACGGAAGGATGCATCAGAGCTTTGATTTTGATGGAGGGATGGCGGGCAGCAAGC
  TGCCAGGGCAGGAGGGCGTGAGGATCCAGCCCATGAGTGAACGCACCCGGAGAAGAGCTACTTCACGCGA
  CGACAACCGCCGTTTCCGACCTCACAGGTCCAGGCGTTCCCGACGCTCTCGCTCCGACAACGCCCTCCAC
  CTGGCCAGCGAACGCGAGGCCATCTCCCGGTTAAAAGATAGGCCCCCTCTGAGAGCCAGGGAGGACTATG
  ACCAATTTATGCGCCAGCGGAGCTTCCAGGAGAGCATGGGGCATGGGTCCCGGAGGGACCTGTACGGCCA
  GTGCCCTAGGACTGTGTCGGACCTGGCTTTGCAGAATGCCTTTGGGGACCGCTGGGGACCCTACTTCGCC
  GAGTATGATTGGTGTTCCACCTGCTCCTCCTCTTCAGAGTCTGACAACGAGGGCTATTTCCTAGGAGAAC
  CCATCCCCCAGCCAGCGCGCCTGCGATACGTCACAAGCGATGAGCTGCTGCACAAATACAGCTCCTACGG
  CCTCCCCAAATCTTCCACATTAGGTGGCAGAGGACAGTTGCACAGCAGGAAAAGACAGAAGAGCAAAAAC
  TGTATCATTTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219763 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVTVMPLEMEKTISKLMFDFQRNSTSDDDSGCALEEYAWVPPGLKPEQVHQYYSCLPEEKVPYVNSPGEK
  LRIKQLLHQLPPHDNEVRYCNSLDEEEKRELKLFSSQRKRENLGRGNVRPFPVTMTGAICEQCGGQINGG
  DIAVFASRAGHGVCWHPPCFVCTVCNELLVDLIYFYQDGKIYCGRHHAECLKPRCAACDEIIFADECTEA
  EGRHWHMKHFCCFECETVLGGQRYIMKEGRPYCCHCFESLYAEYCDTCAQHIGIDQGQMTYDGQHWHATE
  TCFCCAHCKKSLLGRPFLPKQGQIFCSRACSAGEDPNGSDSSDSAFQNARAKESRRSAKIGKNKGKTEEP
  MLNQHSQLQVSSNRLSADVDPLSLQMDMLSLSSQTPSLNRDPIWRSREEPYHYGNKMEQNQTQSPLQLLS
  QCNIRTSYSPGGQGAGAQPEMWGKHFSNPKRSSSLAMTGHAGSFIKECREDYYPGRLRSQESYSDMSSQS
  FSETRGSIQVPKYEEEEEEEGGLSTQQCRTRHPISSLKYTEDMTPTEQTPRGSMESLALSNATGLSADGG
  AKRQEHLSRFSMPDLSKDSGMNVSEKLSNMGTLNSSMQFRSAESVRSLLSAQQYQEMEGNLHQLSNPIGY
  RDLQSHGRMHQSFDFDGGMAGSKLPGQEGVRIQPMSERTRRRATSRDDNRRFRPHRSRRSRRSRSDNALH
  LASEREAISRLKDRPPLRAREDYDQFMRQRSFQESMGHGSRRDLYGQCPRTVSDLALQNAFGDRWGPYFA
  EYDWCSTCSSSSESDNEGYFLGEPIPQPARLRYVTSDELLHKYSSYGLPKSSTLGGRGQLHSRKRQKSKN
  CIIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198859
• ORF Size: 2532 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198859.1, NM_198859.2, NM_198859.3, NP_942559.1
• RefSeq Size: 8322 bp
• RefSeq ORF: 2535 bp
• Locus ID: 166336
• Protein Pathways: Wnt signaling pathway
• MW: 95.6 kDa
• Gene Summary: This gene encodes a homolog of Drosophila prickle. The exact function of this gene is not known, however, studies in mice suggest that it may be involved in seizure prevention. Mutations in this gene are associated with progressive myoclonic epilepsy type 5. [provided by RefSeq, Dec 2011]