CNOT6 (NM_015455) Human Tagged ORF Clone

CAT#: RC219771

CNOT6 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 6 (CNOT6)

Product Images

Western blot validation of overexpression lysate (Cat# LY414536) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219771 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNOT6
• Synonyms: CCR4; Ccr4a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219771 representing NM_015455
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAAAGAAAAATACGAGCCCCCTGACCCTCGGAGGATGTATACAATTATGTCTTCTGAGGAAGCAG
  CAAATGGAAAGAAATCCCACTGGGCAGAGCTTGAAATAAGTGGAAAAGTAAGAAGCTTAAGCGCATCTTT
  GTGGTCACTAACTCACCTGACAGCTTTGCATTTGAGTGACAATTCCCTGTCCCGAATTCCTTCAGACATT
  GCCAAGCTTCACAATCTGGTGTATTTGGACCTGTCATCTAATAAAATTCGTAGCTTACCCGCAGAACTCG
  GAAACATGGTATCACTCAGGGAGCTCCATTTAAATAACAACCTGTTACGAGTTCTACCTTTTGAGCTGGG
  AAAACTGTTTCAGTTGCAGACTTTAGGCCTGAAAGGAAATCCCCTTACCCAGGATATATTGAACCTTTAT
  CAGGAACCAGATGGAACAAGACGGCTGCTGAACTATTTGCTTGATAATTTGTCAGGTACTGCAAAAAGAA
  TTACAACAGAACAACCACCTCCAAGGTCTTGGATTATGTTACAAGAACCAGATAGGACAAGGCCAACTGC
  CTTGTTTTCTGTCATGTGCTATAATGTTCTTTGTGATAAATATGCGACCCGGCAGTTATACGGCTACTGT
  CCATCATGGGCGCTAAACTGGGACTACAGGAAAAAGGCCATTATTCAAGAAATCTTGAGCTGCAATGCTG
  ATATCGTAAGTCTTCAGGAGGTTGAAACGGAACAGTATTACAGTTTTTTTCTGGTAGAGCTGAAAGAACG
  TGGCTATAATGGATTCTTCAGTCCTAAGTCTAGAGCTAGGACAATGTCAGAACAAGAAAGGAAACATGTT
  GATGGCTGTGCAATATTCTTCAAGACAGAAAAATTTACTTTGGTTCAGAAACACACTGTTGAATTTAATC
  AGCTAGCCATGGCAAATTCTGAGGGGTCTGAAGCTATGCTGAACAGAGTCATGACAAAAGATAACATTGG
  GGTTGCAGTACTGCTAGAACTTCGGAAGGAATCGATTGAAATGCCGTCTGGAAAGCCACATCTTGGAACA
  GAAAAACAACTTATTCTTGTGGCTAACGCCCACATGCATTGGGACCCTGAATACTCTGATGTGAAGTTGG
  TACAAACTATGATGTTCCTCTCAGAAGTGAAGAACATTATTGATAAAGCCTCTCGCAACCTCAAATCCAG
  TGTTTTGGGAGAATTTGGAACTATTCCACTTGTGTTATGTGCAGATCTTAATTCTTTGCCAGACTCTGGT
  GTTGTAGAATATTTGAGCACAGGTGGAGTAGAAACAAATCACAAAGACTTTAAGGAGTTGAGGTATAATG
  AAAGTCTCACAAACTTCAGCTGTCATGGGAAGAATGGAACCACCAATGGAAGGATCACTCATGGTTTCAA
  GTTACAGAGTGCCTATGAGAGTGGCCTGATGCCTTACACGAATTACACATTTGATTTCAAGGGTATAATA
  GACTACATTTTCTATTCTAAACCTCAGCTGAACACCTTAGGCATCCTGGGCCCTCTGGACCACCACTGGC
  TGGTTGAGAATAACATCAGTGGCTGCCCGCACCCCCTCATCCCCTCTGACCACTTCTCACTTTTTGCACA
  ACTGGAGCTCTTACTGCCTTTCCTGCCCCAAGTCAACGGCATCCACCTTCCTGGCAGGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219771 representing NM_015455
  Red=Cloning site Green=Tags(s)
  
  MPKEKYEPPDPRRMYTIMSSEEAANGKKSHWAELEISGKVRSLSASLWSLTHLTALHLSDNSLSRIPSDI
  AKLHNLVYLDLSSNKIRSLPAELGNMVSLRELHLNNNLLRVLPFELGKLFQLQTLGLKGNPLTQDILNLY
  QEPDGTRRLLNYLLDNLSGTAKRITTEQPPPRSWIMLQEPDRTRPTALFSVMCYNVLCDKYATRQLYGYC
  PSWALNWDYRKKAIIQEILSCNADIVSLQEVETEQYYSFFLVELKERGYNGFFSPKSRARTMSEQERKHV
  DGCAIFFKTEKFTLVQKHTVEFNQLAMANSEGSEAMLNRVMTKDNIGVAVLLELRKESIEMPSGKPHLGT
  EKQLILVANAHMHWDPEYSDVKLVQTMMFLSEVKNIIDKASRNLKSSVLGEFGTIPLVLCADLNSLPDSG
  VVEYLSTGGVETNHKDFKELRYNESLTNFSCHGKNGTTNGRITHGFKLQSAYESGLMPYTNYTFDFKGII
  DYIFYSKPQLNTLGILGPLDHHWLVENNISGCPHPLIPSDHFSLFAQLELLLPFLPQVNGIHLPGRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015455
• ORF Size: 1671 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015455.2, NM_015455.3, NP_056270.2
• RefSeq Size: 6178
• RefSeq ORF: 1674
• Locus ID: 57472
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• MW: 63.1 kDa
• Gene Summary: This gene encodes the catalytic component of the CCR4-NOT core transcriptional regulation complex. The encoded protein has a 3'-5' RNase activity and prefers polyadenylated substrates. The CCR4-NOT complex plays a role in many cellular processes, including miRNA-mediated repression, mRNA degradation, and transcriptional regulation. [provided by RefSeq, Dec 2014]