SPECC1L (NM_015330) Human Tagged ORF Clone

CAT#: RC219883

SPECC1L (Myc-DDK-tagged)-Human sperm antigen with calponin homology and coiled-coil domains 1-like (SPECC1L), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SPECC1L
• Synonyms: CYTSA; GBBB2; OBLFC1; TBHS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219883 representing NM_015330
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAAGCAAGCAGGAGTGTTGGCTCAGTGCCTAAAGTGTCTGCAATAAGTAAAACGCAAACAGCAG
  AAAAAATTAAACCTGAAAACAGCTCTTCAGCATCTACGGGAGGCAAACTTGTAAAACCTGGAACAGCAGC
  ATCATTGTCAAAGACCAAGAGCAGTGATGACCTTTTAGCTGGAATGGCCGGAGGGGTAACGGTGACTAAT
  GGTGTTAAAGGAAAGAAAAGCACCTGCCCATCTGCAGCACCTTCAGCATCTGCCCCTGCCATGACCACCG
  TGGAGAACAAATCCAAGATTAGCACAGGCACAGCTTCTTCAACCAAGCGGAGCACTTCTACAGGTAATAA
  AGAATCCAGTTCTACTAGAGAAAGATTACGTGAACGTACCCGATTAAACCAGAGCAAAAAACTACCTTCT
  GCAGGTCAGGGAGCTAATGACATGGCATTGGCCAAACGTTCCCGCAGTCGAACTGCTACAGAATGTGACG
  TTCGTATGAGCAAGTCTAAGTCAGACAATCAGATCAGTGACAGAGCTGCTTTGGAGGCCAAAGTGAAGGA
  TCTTCTCACGCTGGCAAAAACCAAAGACGTAGAAATTTTACATTTGAGAAATGAACTGCGAGACATGCGT
  GCCCAGCTGGGCATTAATGAGGATCATTCTGAGGGTGATGAAAAATCTGAGAAGGAAACTATTATGGCTC
  ACCAGCCGACTGATGTGGAGTCCACTTTATTGCAGTTGCAGGAACAGAATACTGCCATCCGTGAAGAACT
  CAACCAGCTGAAAAATGAAAACAGAATGTTAAAGGACAGGTTGAATGCATTGGGCTTTTCCCTAGAGCAG
  AGGTTAGACAATTCTGAAAAACTGTTTGGCTATCAGTCCCTGAGCCCAGAAATCACCCCTGGTAACCAGA
  GCGATGGAGGAGGAACTCTGACTTCTTCAGTGGAAGGCTCTGCCCCTGGCTCAGTGGAGGATCTCTTGAG
  TCAGGATGAAAATACACTAATGGACCATCAGCACAGTAACTCCATGGACAATTTAGACAGTGAGTGCAGT
  GAGGTCTACCAGCCCCTCACATCGAGCGATGATGCGCTGGATGCACCATCCTCCTCAGAGTCGGAAGGCA
  TCCTCAGCATAGAGCGCTCCCGGAAGGGGAGCAGCGGGAATGCCAGTGAAGTGTCCGTGGCTTGCCTGAC
  TGAACGGATACACCAGATGGAAGAGAACCAACACAGTACAAGTGAGGAACTCCAGGCAACCCTGCAAGAG
  CTAGCTGATTTACAGCAGATTACCCAGGAACTGAATAGTGAAAACGAAAGGCTTGGAGAAGAGAAGGTTA
  TTCTGATGGAGTCTTTATGTCAGCAGAGCGATAAGTTGGAACACTTTAGTCGACAGATTGAATACTTCCG
  CTCTCTTCTAGATGAGCATCACATTTCTTATGTCATAGATGAAGATGTAAAAAGTGGGCGCTATATGGAA
  TTAGAGCAACGTTACATGGACCTCGCTGAGAATGCCCGTTTTGAACGGGAGCAGCTTCTTGGTGTCCAGC
  AGCATTTAAGCAATACTTTGAAAATGGCAGAACAAGACAATAAGGAAGCTCAAGAAATGATAGGGGCACT
  CAAAGAACGCAGTCACCATATGGAGCGAATTATTGAGTCTGAGCAGAAAGGAAAAGCAGCCTTGGCAGCC
  ACGTTAGAGGAATACAAAGCCACAGTGGCCAGTGACCAGATAGAGATGAATCGCCTGAAGGCTCAGCTGG
  AGAATGAAAAGCAGAAAGTGGCAGAGCTGTATTCTATCCATAACTCTGGAGACAAATCTGATATTCAGGA
  CCTCCTGGAGAGTGTCAGGCTGGACAAAGAAAAAGCAGAGACTTTGGCTAGTAGCTTGCAGGAAGATCTG
  GCTCATACCCGAAATGATGCCAATCGATTACAGGATGCCATTGCTAAGGTAGAGGATGAATACCGAGCCT
  TCCAAGAAGAAGCTAAGAAACAAATTGAAGATTTGAATATGACGTTAGAAAAATTAAGATCAGACCTGGA
  TGAAAAAGAAACAGAAAGGAGTGACATGAAAGAAACCATCTTTGAACTTGAAGATGAAGTAGAACAACAT
  CGTGCTGTGAAACTTCATGACAACCTCATTATTTCTGATCTAGAGAATACAGTTAAAAAACTCCAGGACC
  AAAAGCACGACATGGAAAGAGAAATAAAGACACTCCACAGAAGACTTCGGGAAGAATCTGCGGAATGGCG
  GCAGTTTCAGGCTGATCTCCAGACTGCAGTAGTCATTGCAAATGACATTAAATCTGAAGCCCAAGAGGAG
  ATTGGTGATCTAAAGCGCCGGTTACATGAGGCTCAAGAAAAAAATGAGAAACTCACAAAAGAATTGGAGG
  AAATAAAGTCACGCAAGCAAGAGGAGGAGCGAGGCCGGGTATACAATTACATGAATGCCGTTGAGAGAGA
  TTTGGCAGCCTTAAGGCAGGGAATGGGACTGAGTAGAAGGTCCTCGACTTCCTCAGAGCCAACTCCTACA
  GTAAAAACCCTCATCAAGTCCTTTGACAGTGCATCTCAAGTACCAAACCCTGCTGCAGCTGCAATTCCTC
  GAACGCCCCTGAGCCCAAGTCCTATGAAAACCCCTCCTGCAGCAGCTGTGTCCCCTATGCAGAGACATTC
  CATAAGTGGACCAATCTCAACATCCAAACCCCTGACAGCCCTGTCAGATAAGAGACCAAACTATGGGGAA
  ATCCCTGTTCAAGAGCATCTGTTAAGAACATCTTCAGCCAGCCGGCCTGCTTCCCTGCCAAGAGTGCCTG
  CGATGGAAAGTGCCAAGACCCTCTCAGTGTCTCGACGAAGTAGTGAAGAAATGAAACGGGACATTTCTGC
  ACAGGAGGGAGCGTCGCCAGCCTCTCTGATGGCTATGGGAACCACGTCTCCACAGCTTTCCCTGTCCTCT
  TCTCCAACGGCATCTGTGACTCCCACCACCCGAAGCCGAATAAGAGAAGAAAGGAAAGACCCTCTCTCAG
  CATTGGCCAGAGAATATGGAGGATCAAAGAGGAACGCCTTGCTGAAGTGGTGTCAGAAGAAAACAGAAGG
  CTATCAGAATATTGACATTACAAACTTCAGCAGCAGCTGGAATGATGGGCTGGCCTTCTGTGCCCTCCTG
  CATACATATCTCCCTGCCCACATTCCATATCAAGAACTGAACAGCCAGGATAAGAGAAGGAACTTCATGC
  TGGCTTTCCAGGCAGCTGAAAGTGTCGGCATCAAATCCACACTGGACATTAATGAAATGGTACGGACTGA
  ACGACCCGACTGGCAGAACGTGATGCTGTATGTGACGGCGATCTACAAGTACTTTGAGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219883 representing NM_015330
  Red=Cloning site Green=Tags(s)
  
  MKKASRSVGSVPKVSAISKTQTAEKIKPENSSSASTGGKLVKPGTAASLSKTKSSDDLLAGMAGGVTVTN
  GVKGKKSTCPSAAPSASAPAMTTVENKSKISTGTASSTKRSTSTGNKESSSTRERLRERTRLNQSKKLPS
  AGQGANDMALAKRSRSRTATECDVRMSKSKSDNQISDRAALEAKVKDLLTLAKTKDVEILHLRNELRDMR
  AQLGINEDHSEGDEKSEKETIMAHQPTDVESTLLQLQEQNTAIREELNQLKNENRMLKDRLNALGFSLEQ
  RLDNSEKLFGYQSLSPEITPGNQSDGGGTLTSSVEGSAPGSVEDLLSQDENTLMDHQHSNSMDNLDSECS
  EVYQPLTSSDDALDAPSSSESEGILSIERSRKGSSGNASEVSVACLTERIHQMEENQHSTSEELQATLQE
  LADLQQITQELNSENERLGEEKVILMESLCQQSDKLEHFSRQIEYFRSLLDEHHISYVIDEDVKSGRYME
  LEQRYMDLAENARFEREQLLGVQQHLSNTLKMAEQDNKEAQEMIGALKERSHHMERIIESEQKGKAALAA
  TLEEYKATVASDQIEMNRLKAQLENEKQKVAELYSIHNSGDKSDIQDLLESVRLDKEKAETLASSLQEDL
  AHTRNDANRLQDAIAKVEDEYRAFQEEAKKQIEDLNMTLEKLRSDLDEKETERSDMKETIFELEDEVEQH
  RAVKLHDNLIISDLENTVKKLQDQKHDMEREIKTLHRRLREESAEWRQFQADLQTAVVIANDIKSEAQEE
  IGDLKRRLHEAQEKNEKLTKELEEIKSRKQEEERGRVYNYMNAVERDLAALRQGMGLSRRSSTSSEPTPT
  VKTLIKSFDSASQVPNPAAAAIPRTPLSPSPMKTPPAAAVSPMQRHSISGPISTSKPLTALSDKRPNYGE
  IPVQEHLLRTSSASRPASLPRVPAMESAKTLSVSRRSSEEMKRDISAQEGASPASLMAMGTTSPQLSLSS
  SPTASVTPTTRSRIREERKDPLSALAREYGGSKRNALLKWCQKKTEGYQNIDITNFSSSWNDGLAFCALL
  HTYLPAHIPYQELNSQDKRRNFMLAFQAAESVGIKSTLDINEMVRTERPDWQNVMLYVTAIYKYFET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015330
• ORF Size: 3351 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015330.1, NM_015330.2, NM_015330.3, NM_015330.4, NP_056145.1, NP_056145.4
• RefSeq Size: 6255
• RefSeq ORF: 3354
• Locus ID: 23384
• MW: 124.4 kDa
• Gene Summary: This gene encodes a coiled-coil domain containing protein. The encoded protein may play a critical role in actin-cytoskeletal reorganization during facial morphogenesis. Mutations in this gene are a cause of oblique facial clefting-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A read-through transcript composed of SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and the downstream ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. [provided by RefSeq, Jun 2013]