ZFYVE27 (NM_001002261) Human Tagged ORF Clone

CAT#: RC219897

ZFYVE27 (Myc-DDK-tagged)-Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY424194) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219897 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZFYVE27 protein (Cat# TP319897). The protein was produced from HEK293T cells transfected with ZFYVE27 cDNA clone (Cat# RC219897) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZFYVE27
• Synonyms: PROTRUDIN; SPG33
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219897 representing NM_001002261
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGACATCAGAACGTGAGGGGAGTGGGCCGGAGCTGAGCCCCAGCGTGATGCCCGAGGCTCCCCTGG
  AGTCTCCACCTTTTCCTACCAAGTCCCCAGCGTTTGACCTTTTCAACTTGGTTCTCTCCTACAAGAGGCT
  GGAGATCTACCTGGAACCCTTGAAGGATGCAGGTGATGGTGTTCGATACTTGCTCAGGTGGCAGATGCCT
  TTGTGTTCCTTGCTGACCTGCCTGGGCCTCAACGTCTTGTTCCTCACTTTGAATGAGGGTGCATGGTACT
  CAGTAGGTGCCCTGATGATTTCAGTGCCCGCCCTGCTGGGCTACCTTCAGGAGGTTTGCCGGGCACGGCT
  GCCTGATTCCGAGCTGATGCGGAGGAAGTATCATAGCGTGAGGCAGGAGGACCTGCAGAGAGGTCGCCTG
  TCTCGTCCCGAGGCCGTGGCTGAGGTGAAGAGCTTCTTGATCCAGCTGGAGGCCTTCCTGAGCCGCCTGT
  GCTGCACATGTGAAGCCGCCTACCGCGTGCTGCACTGGGAGAACCCCGTCGTGTCCTCACAGTTCTATGG
  GGCTCTTCTGGGCACAGTCTGCATGCTGTATTTGCTGCCACTCTGCTGGGTTCTCACCCTTTTAAACAGC
  ACGCTCTTTCTGGGGAATGTGGAGTTCTTCCGAGTTGTGTCTGAGTACAGGGCATCTCTGCAGCAGAGGA
  TGAACCCAAAGCAGGAAGAGCATGCCTTTGAGAGTCCTCCACCACCAGATGTTGGGGGGAAGGATGGTCT
  GATGGACAGCACGCCTGCCCTCACACCCACGGAGAGTCTCTCTTCCCAGGACCTCACACCGGGCAGCGTG
  GAGGAGGCTGAGGAGGCTGAGCCAGATGAAGAGTTTAAAGATGCGATTGAGGAGACCCACTTGGTGGTGC
  TGGAGGATGATGAGGGCGCCCCGTGCCCAGCAGAGGATGAGCTGGCCCTGCAGGACAACGGGTTCCTGAG
  CAAGAATGAGGTGCTGCGCAGCAAGGTGTCTCGGCTCACGGAGCGGCTCCGCAAGCGCTACCCCACCAAC
  AACTTCGGGAACTGCACGGGCTGCTCGGCCACCTTCTCAGTGCTGAAGAAGAGGCGGAGCTGCAGTAATT
  GTGGAAACAGCTTCTGCTCTCGATGCTGCTCCTTCAAGGTGCCCAAGTCCTCCATGGGGGCCACAGCCCC
  TGAAGCCCAGAGGGAGACTGTGTTTGTGTGTGCCTCGTGTAACCAGACCTTGAGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219897 representing NM_001002261
  Red=Cloning site Green=Tags(s)
  
  MQTSEREGSGPELSPSVMPEAPLESPPFPTKSPAFDLFNLVLSYKRLEIYLEPLKDAGDGVRYLLRWQMP
  LCSLLTCLGLNVLFLTLNEGAWYSVGALMISVPALLGYLQEVCRARLPDSELMRRKYHSVRQEDLQRGRL
  SRPEAVAEVKSFLIQLEAFLSRLCCTCEAAYRVLHWENPVVSSQFYGALLGTVCMLYLLPLCWVLTLLNS
  TLFLGNVEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTESLSSQDLTPGSV
  EEAEEAEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTN
  NFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002261
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002261.1, NM_001002261.2, NM_001002261.3, NP_001002261.1
• RefSeq Size: 3045 bp
• RefSeq ORF: 1251 bp
• Locus ID: 118813
• Cytogenetics: 10q24.2
• Protein Families: Transmembrane
• MW: 46.2 kDa
• Gene Summary: This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. [provided by RefSeq, Mar 2010]