ZFYVE27 (NM_001002261) Human Untagged Clone

CAT#: SC300426

ZFYVE27 (untagged)-Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 1


  "NM_001002261" in other vectors (6)

Reconstitution Protocol

USD 710.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ZFYVE27
Synonyms PROTRUDIN; SPG33
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NCBI ORF sequence for NM_001002261, the custom clone sequence may differ by one or more nucleotides


ATGCAGACATCAGAACGTGAGGGGAGTGGGCCGGAGCTGAGCCCCAGCGTGATGCCCGAGGCTCCCCTGG
AGTCTCCACCTTTTCCTACCAAGTCCCCAGCGTTTGACCTTTTCAACTTGGTTCTCTCCTACAAGAGGCT
GGAGATCTACCTGGAACCCTTGAAGGATGCAGGTGATGGTGTTCGATACTTGCTCAGGTGGCAGATGCCT
TTGTGTTCCTTGCTGACCTGCCTGGGCCTCAACGTCTTGTTCCTCACTTTGAATGAGGGTGCATGGTACT
CAGTAGGTGCCCTGATGATTTCAGTGCCCGCCCTGCTGGGCTACCTTCAGGAGGTTTGCCGGGCACGGCT
GCCTGATTCCGAGCTGATGCGGAGGAAGTATCATAGCGTGAGGCAGGAGGACCTGCAGAGAGGTCGCCTG
TCTCGTCCCGAGGCCGTGGCTGAGGTGAAGAGCTTCTTGATCCAGCTGGAGGCCTTCCTGAGCCGCCTGT
GCTGCACATGTGAAGCCGCCTACCGCGTGCTGCACTGGGAGAACCCCGTCGTGTCCTCACAGTTCTATGG
GGCTCTTCTGGGCACAGTCTGCATGCTGTATTTGCTGCCACTCTGCTGGGTTCTCACCCTTTTAAACAGC
ACGCTCTTTCTGGGGAATGTGGAGTTCTTCCGAGTTGTGTCTGAGTACAGGGCATCTCTGCAGCAGAGGA
TGAACCCAAAGCAGGAAGAGCATGCCTTTGAGAGTCCTCCACCACCAGATGTTGGGGGGAAGGATGGTCT
GATGGACAGCACGCCTGCCCTCACACCCACGGAGAGTCTCTCTTCCCAGGACCTCACACCGGGCAGCGTG
GAGGAGGCTGAGGAGGCTGAGCCAGATGAAGAGTTTAAAGATGCGATTGAGGAGACCCACTTGGTGGTGC
TGGAGGATGATGAGGGCGCCCCGTGCCCAGCAGAGGATGAGCTGGCCCTGCAGGACAACGGGTTCCTGAG
CAAGAATGAGGTGCTGCGCAGCAAGGTGTCTCGGCTCACGGAGCGGCTCCGCAAGCGCTACCCCACCAAC
AACTTCGGGAACTGCACGGGCTGCTCGGCCACCTTCTCAGTGCTGAAGAAGAGGCGGAGCTGCAGTAATT
GTGGAAACAGCTTCTGCTCTCGATGCTGCTCCTTCAAGGTGCCCAAGTCCTCCATGGGGGCCACAGCCCC
TGAAGCCCAGAGGGAGACTGTGTTTGTGTGTGCCTCGTGTAACCAGACCTTGAGCAAGTGA


Restriction Sites SgfI-MluI     
ACCN NM_001002261
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001002261.3, NP_001002261.1
RefSeq Size 2869 bp
RefSeq ORF 1251 bp
Locus ID 118813
Cytogenetics 10q24.2
Protein Families Transmembrane
Gene Summary This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. [provided by RefSeq, Mar 2010]
Transcript Variant: This variant (1) encodes the longest isoform (a).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.