ZFYVE27 (NM_001002261) Human Untagged Clone

CAT#: SC300426

ZFYVE27 (untagged)-Human zinc finger, FYVE domain containing 27 (ZFYVE27), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZFYVE27
• Synonyms: PROTRUDIN; SPG33
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001002261, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGACATCAGAACGTGAGGGGAGTGGGCCGGAGCTGAGCCCCAGCGTGATGCCCGAGGCTCCCCTGG
  AGTCTCCACCTTTTCCTACCAAGTCCCCAGCGTTTGACCTTTTCAACTTGGTTCTCTCCTACAAGAGGCT
  GGAGATCTACCTGGAACCCTTGAAGGATGCAGGTGATGGTGTTCGATACTTGCTCAGGTGGCAGATGCCT
  TTGTGTTCCTTGCTGACCTGCCTGGGCCTCAACGTCTTGTTCCTCACTTTGAATGAGGGTGCATGGTACT
  CAGTAGGTGCCCTGATGATTTCAGTGCCCGCCCTGCTGGGCTACCTTCAGGAGGTTTGCCGGGCACGGCT
  GCCTGATTCCGAGCTGATGCGGAGGAAGTATCATAGCGTGAGGCAGGAGGACCTGCAGAGAGGTCGCCTG
  TCTCGTCCCGAGGCCGTGGCTGAGGTGAAGAGCTTCTTGATCCAGCTGGAGGCCTTCCTGAGCCGCCTGT
  GCTGCACATGTGAAGCCGCCTACCGCGTGCTGCACTGGGAGAACCCCGTCGTGTCCTCACAGTTCTATGG
  GGCTCTTCTGGGCACAGTCTGCATGCTGTATTTGCTGCCACTCTGCTGGGTTCTCACCCTTTTAAACAGC
  ACGCTCTTTCTGGGGAATGTGGAGTTCTTCCGAGTTGTGTCTGAGTACAGGGCATCTCTGCAGCAGAGGA
  TGAACCCAAAGCAGGAAGAGCATGCCTTTGAGAGTCCTCCACCACCAGATGTTGGGGGGAAGGATGGTCT
  GATGGACAGCACGCCTGCCCTCACACCCACGGAGAGTCTCTCTTCCCAGGACCTCACACCGGGCAGCGTG
  GAGGAGGCTGAGGAGGCTGAGCCAGATGAAGAGTTTAAAGATGCGATTGAGGAGACCCACTTGGTGGTGC
  TGGAGGATGATGAGGGCGCCCCGTGCCCAGCAGAGGATGAGCTGGCCCTGCAGGACAACGGGTTCCTGAG
  CAAGAATGAGGTGCTGCGCAGCAAGGTGTCTCGGCTCACGGAGCGGCTCCGCAAGCGCTACCCCACCAAC
  AACTTCGGGAACTGCACGGGCTGCTCGGCCACCTTCTCAGTGCTGAAGAAGAGGCGGAGCTGCAGTAATT
  GTGGAAACAGCTTCTGCTCTCGATGCTGCTCCTTCAAGGTGCCCAAGTCCTCCATGGGGGCCACAGCCCC
  TGAAGCCCAGAGGGAGACTGTGTTTGTGTGTGCCTCGTGTAACCAGACCTTGAGCAAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001002261
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002261.3, NP_001002261.1
• RefSeq Size: 2869 bp
• RefSeq ORF: 1251 bp
• Locus ID: 118813
• Cytogenetics: 10q24.2
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear. [provided by RefSeq, Mar 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (a).