LPCAT1 (NM_024830) Human Tagged ORF Clone
CAT#: RC220083
LPCAT1 (Myc-DDK-tagged)-Human lysophosphatidylcholine acyltransferase 1 (LPCAT1)
"NM_024830" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | LPCAT1 |
Synonyms | AGPAT9; AGPAT10; AYTL2; lpcat; LPCAT-1; lysoPAFAT; PFAAP3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220083 representing NM_024830
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTGCGGGGATGCGGACCCCGGGCCGCCCCTGCCTCCAGCGCAGGGGCCAGCGACGCTCGGCTGC TGGCGCCCCCGGGGCGGAACCCCTTCGTGCACGAGCTGCGCCTCAGCGCCCTGCAGAAGGCCCAGGTGGC CCTCATGACACTGACGCTCTTCCCGGTCCGGCTCCTGGTTGCCGCTGCCATGATGCTGCTGGCCTGGCCC CTCGCACTTGTCGCATCCCTGGGCTCTGCGGAGAAGGAACCCGAGCAGCCCCCGGCCCTGTGGAGGAAGG TTGTGGACTTCCTGCTGAAGGCCATCATGCGCACCATGTGGTTCGCCGGCGGCTTCCACCGGGTGGCCGT GAAGGGGCGGCAGGCGCTGCCCACCGAGGCGGCCATCCTCACGCTCGCGCCTCACTCGTCCTACTTCGAC GCCATCCCTGTGACCATGACGATGTCCTCCATCGTGATGAAGGCAGAGAGCAGAGACATCCCGATCTGGG GAACTCTGATCCAGTATATACGGCCTGTGTTCGTGTCCCGGTCAGACCAGGATTCTCGCAGGAAAACAGT AGAAGAAATCAAGAGACGGGCGCAGTCCAACGGAAAGTGGCCACAGATAATGATTTTTCCAGAAGGAACT TGTACAAACAGGACCTGCCTAATTACCTTCAAACCTGGTGCATTCATCCCTGGAGCGCCCGTCCAGCCTG TGGTTTTACGATATCCAAATAAACTGGACACCATCACATGGACGTGGCAAGGACCTGGAGCGCTGGAAAT CCTGTGGCTCACGCTGTGTCAGTTTCACAACCAAGTGGAAATCGAGTTCCTTCCTGTGTACAGCCCTTCT GAGGAGGAGAAGAGGAACCCCGCGCTGTATGCCAGCAACGTGCGGCGAGTCATGGCCGAGGCCTTGGGTG TCTCCGTGACTGACTACACGTTCGAGGACTGCCAGCTGGCCCTGGCGGAAGGACAGCTCCGTCTCCCCGC TGACACTTGCCTTTTAGAATTTGCCAGGCTCGTGCGGGGCCTCGGGCTAAAACCAGAAAAGCTTGAAAAA GATCTGGACAGATACTCAGAAAGAGCCAGGATGAAGGGAGGAGAGAAGATAGGTATTGCGGAGTTTGCCG CCTCCCTGGAAGTCCCCGTTTCTGACTTGCTGGAAGACATGTTTTCACTGTTCGACGAGAGCGGCAGCGG CGAGGTGGACCTGCGAGAGTGTGTGGTTGCCCTGTCTGTCGTCTGCCGGCCGGCCCGGACCCTGGACACC ATCCAGCTGGCTTTCAAGATGTACGGAGCGCAAGAGGACGGCAGCGTCGGCGAAGGTGACCTGTCCTGCA TCCTCAAGACGGCCCTGGGGGTGGCAGAGCTCACCGTGACCGACCTATTCCGAGCCATTGACCAAGAGGA GAAGGGGAAGATCACATTCGCTGACTTCCACAGGTTTGCAGAAATGTACCCTGCCTTCGCAGAGGAATAC CTGTACCCGGATCAGACACATTTCGAAAGCTGTGCAGAGACCTCACCTGCGCCAATCCCAAACGGCTTCT GTGCCGATTTCAGCCCGGAAAACTCAGACGCTGGGCGGAAGCCTGTTCGCAAGAAGCTGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220083 representing NM_024830
Red=Cloning site Green=Tags(s) MRLRGCGPRAAPASSAGASDARLLAPPGRNPFVHELRLSALQKAQVALMTLTLFPVRLLVAAAMMLLAWP LALVASLGSAEKEPEQPPALWRKVVDFLLKAIMRTMWFAGGFHRVAVKGRQALPTEAAILTLAPHSSYFD AIPVTMTMSSIVMKAESRDIPIWGTLIQYIRPVFVSRSDQDSRRKTVEEIKRRAQSNGKWPQIMIFPEGT CTNRTCLITFKPGAFIPGAPVQPVVLRYPNKLDTITWTWQGPGALEILWLTLCQFHNQVEIEFLPVYSPS EEEKRNPALYASNVRRVMAEALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGLKPEKLEK DLDRYSERARMKGGEKIGIAEFAASLEVPVSDLLEDMFSLFDESGSGEVDLRECVVALSVVCRPARTLDT IQLAFKMYGAQEDGSVGEGDLSCILKTALGVAELTVTDLFRAIDQEEKGKITFADFHRFAEMYPAFAEEY LYPDQTHFESCAETSPAPIPNGFCADFSPENSDAGRKPVRKKLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024830 |
ORF Size | 1602 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024830.3, NM_024830.4, NP_079106.3 |
RefSeq Size | 3994 bp |
RefSeq ORF | 1605 bp |
Locus ID | 79888 |
Domains | EFh, Acyltransferase |
Protein Families | Transmembrane |
MW | 59 kDa |
Gene Summary | This gene encodes a member of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family of proteins. The encoded enzyme plays a role in phospholipid metabolism, specifically in the conversion of lysophosphatidylcholine to phosphatidylcholine in the presence of acyl-CoA. This process is important in the synthesis of lung surfactant and platelet-activating factor (PAF). Elevated expression of this gene may contribute to the progression of oral squamous cell, prostate, breast, and other human cancers. [provided by RefSeq, Sep 2016] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC128138 | LPCAT1 (untagged)-Human lysophosphatidylcholine acyltransferase 1 (LPCAT1) |
USD 900.00 |
|
RG220083 | LPCAT1 (GFP-tagged) - Human lysophosphatidylcholine acyltransferase 1 (LPCAT1) |
USD 540.00 |
|
RC220083L1 | Lenti ORF clone of Human lysophosphatidylcholine acyltransferase 1 (LPCAT1), Myc-DDK-tagged |
USD 852.00 |
|
RC220083L2 | Lenti ORF clone of Human lysophosphatidylcholine acyltransferase 1 (LPCAT1), mGFP tagged |
USD 690.00 |
|
RC220083L3 | Lenti ORF clone of Human lysophosphatidylcholine acyltransferase 1 (LPCAT1), Myc-DDK-tagged |
USD 852.00 |
|
RC220083L4 | Lenti ORF clone of Human lysophosphatidylcholine acyltransferase 1 (LPCAT1), mGFP tagged |
USD 690.00 |
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