WDR52 (CFAP44) (NM_018338) Human Tagged ORF Clone

CAT#: RC220092

WDR52 (Myc-DDK-tagged)-Human WD repeat domain 52 (WDR52), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY413131) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220092 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CFAP44 protein (Cat# TP320092). The protein was produced from HEK293T cells transfected with CFAP44 cDNA clone (Cat# RC220092) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CFAP44
• Synonyms: SPGF20; WDR52
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220092 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAACCAGATGATCAGGATACTGATGGGGAGAAATCAGTTACATCAAAGAGTGATGGGAAGAAGT
  CTCTGAGGTCTTCTAAATCAGAATCAAGATCTCCTGTTCAAGAAGATAACACATTTTTAGAAGATGACAC
  AGATGAAACATTTACCAAAGGGGAAGGATCATATTTAGAAGAAGACTCAGATGAGGAACGTTTGGAAGGA
  AGTTTGAGTTCATTTCAGTATGGTGATTTGCAAAGCACTACAGTACCTCAGCAAACCCCTGCTCCAGCTG
  TGGAAGAAGCAGAGGAGGAAGTTAAGAAGAAAATATCAGAGAGCTTCTTCTATGATTATATGGAGCTTGC
  TTCGATGCCTTTTGTGACTCTGGATTCAAACATACCACTGGATCTTCTCACACTTGTACATTCTTTTGGT
  TATGACTGTAGAAAGCGAGCCAACCTACAACTTCTGGACGACAGTATCGCCATATACATAGCTGGGAACC
  AACTGATCTTTCTGAATTTGAAAACCAAGGAACAGATCTACCTGCGAAGTAGCAGTGGTGAAGGAATTGG
  CGTCATTGGGGTTCATCCACATAAAACTTATTTCACAGTAGCTGAAAAAGGGAGTTTTCCAGATATTATC
  ATCTATGAATATCCTTCTCTGAGACCATACAGAGTCCTTCGAGATGGGACTGAGAAGGGATATGCTTATG
  TGGACTTTAACTACAGCGGTAACTTGCTGGCCTCTGTTGGTAGTAACCCTGACTACACACTGACTATCTG
  GAACTGGAAAGAAGAACAACCCATACTAAGGACAAAAGCTTTTTCTCAGGAAGTTTTTAAGGTTACTTTC
  AATCCTGATAAGGAAGAGCAGCTTACTACATCGGGATCAGGCCACATCAAGTTCTGGGAAATGGCTTTTA
  CGTTCACCGGTCTCAAGCTGCAGGGATCACTAGGTCGATTTGGCAAAACAATCACTACTGATATAGAAGG
  CTACATGGAGCTCCCAGATGGGAAGGTGCTCTCAGGGTCAGAATGGGGCAACATGCTGCTTTGGGAAGGT
  GGTCTGATCAAAGTGGAGCTCTGTCGAGGGACAAGCAAGTCATGTCACAATGGTCCCATTAACCAGATAA
  TGCTGTATGAGGGTGAAGTTATCACTGTTGGGTCAGATGGATATGTTAGGATATGGGATTTTGAGACAAT
  AGACACTGCTGATGTAATAGATGAGACTGGATTGTTGGAGATTGAGCCTATTAATGAACTTCAAGTAGAC
  AAGAATGTGAATCTCTTCTCTATGATAAAAATGAATGAAACTGGAAATAACTTTTGGTTGGCTCAGGATG
  CCAATGGAGCCATATGGAAGCTTGACCTTAGTTTTTCAAATATTACCCAGGACCCAGAATGCCTCTTCTC
  CTTCCATTCTGGAGCTATTGAAGCCGTGGCTGTTTCTCCTCTCACTTATCTCATGGCCACAACTGCCTTG
  GACTGCTCTGTTCGAATCTATGATTTTGCTAGCAAAACTCCTTTGGCCCAGATGAAATTCAAACAAGGAG
  GTACTGCCCTTGTTTGGGTACCCCGAATGGTAAACTTCACTGGAGCACAAATTATTGTAGGATTTGAAGA
  TGGAGTTGTTCGAATTCTTGAACTTTATGATCCAAAAGGGCTCACGATTTTTGCGGGACGGAAGAAAATT
  TTGGATGCTGATATTCAGTTGAAACAGGTTTTCAAACCCCATACTGCTTGTGTCACTGCTTTAGCTTATG
  AACGTGATGGGGAAATTCTAGCCACAGGGAGTAAAGATCAAACTGTTTTCTTCTTTGAAGTGGAAAGGGA
  TTATAAGCCGATTGGTTATATTAATACTCCTGGACCTGTGTGTCAGTTAATGTGGTCTCCCATGTCTCAT
  CCTGAAAGTACTTTACTAATTATCTGTGAAAATGGCTATATTCTTGAAGCTCCACTTCCAACCATAAAGC
  AAGAAGAGGATGATCATGATGTAGTCTCCTATGAAATCAAAGACATGTGCATAAAATGTTTCCATTTTTC
  AAGTGTCAAATCTAAGATTCTGAGATTAATAGAAATTGAAAAGAGGGAGAGACAAAGGGAGTTGAAGGAG
  AAAATAAGGGAAGAAAGGAGGAACAAGCTAGCAGCAGAGATGGGAGAAGATGGAGAAAAAGAATTTCAGG
  AGGAGGAAGAGGAGAAAGAGGAGGAGGAGGAGGAAGAAGAGCCATTACCTGAAATATTTATTCCGTCAAC
  CCCCTCTCCCATCCTCTGTGGATTTTACTCAGAGCCAGGGAAGTTCTGGGTTTCTTTGGGTGGCTATGAT
  TCTGGTTTTCTATATCACTGTGAGTTCCCCCCTTGTGATGAAAGCAGTGATTTCAAAGAACAAAAAGATG
  AACCTATTGATGTCCGTTATCTTGCGGATACAGAGGACAATCCCATCCAAACTATCACTTTCAACATTAA
  CAAAGTTATGATGTTTTGTGGAATGAAAAATGGAGCAATTCGAGTCTATGTCCTAAATCAAAATGATCCT
  TCATTGACCAGTTTGGTGGACTACTGGCACTTCAATATGCATGACAATAATTATGGATGTATTAAAAGTA
  TTGCTAATAGCTTTGATGATCGTTTCTTGGTGACTGCTGGAGCAGATGGCAATATCTTTGTTTTCAACAT
  TTTTTCTGAATTTATGCTAAGGAAAGACATGAAGGCCAAAGTTCCATCTCCCAGGTTTGGAATTGAAACA
  GAGCCAATTCCAGAAGACATTGAAGATCCCAAAGCCTACAGTATCGAGAATGCTAGGAGAAAAAGAGAAC
  ATGACAAGTTAATGAAAGAAGTGGGAGAAATAAAGGCACGGAAGAGAGAGCAAATCAAAGCTTTGAGGAG
  TGAATTTTGTAATCTATTAGAAATGAATGAAAAATTACCAAAGCATATGCAGTTTAAACGAACAGTAAGC
  CTAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220092 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKEPDDQDTDGEKSVTSKSDGKKSLRSSKSESRSPVQEDNTFLEDDTDETFTKGEGSYLEEDSDEERLEG
  SLSSFQYGDLQSTTVPQQTPAPAVEEAEEEVKKKISESFFYDYMELASMPFVTLDSNIPLDLLTLVHSFG
  YDCRKRANLQLLDDSIAIYIAGNQLIFLNLKTKEQIYLRSSSGEGIGVIGVHPHKTYFTVAEKGSFPDII
  IYEYPSLRPYRVLRDGTEKGYAYVDFNYSGNLLASVGSNPDYTLTIWNWKEEQPILRTKAFSQEVFKVTF
  NPDKEEQLTTSGSGHIKFWEMAFTFTGLKLQGSLGRFGKTITTDIEGYMELPDGKVLSGSEWGNMLLWEG
  GLIKVELCRGTSKSCHNGPINQIMLYEGEVITVGSDGYVRIWDFETIDTADVIDETGLLEIEPINELQVD
  KNVNLFSMIKMNETGNNFWLAQDANGAIWKLDLSFSNITQDPECLFSFHSGAIEAVAVSPLTYLMATTAL
  DCSVRIYDFASKTPLAQMKFKQGGTALVWVPRMVNFTGAQIIVGFEDGVVRILELYDPKGLTIFAGRKKI
  LDADIQLKQVFKPHTACVTALAYERDGEILATGSKDQTVFFFEVERDYKPIGYINTPGPVCQLMWSPMSH
  PESTLLIICENGYILEAPLPTIKQEEDDHDVVSYEIKDMCIKCFHFSSVKSKILRLIEIEKRERQRELKE
  KIREERRNKLAAEMGEDGEKEFQEEEEEKEEEEEEEEPLPEIFIPSTPSPILCGFYSEPGKFWVSLGGYD
  SGFLYHCEFPPCDESSDFKEQKDEPIDVRYLADTEDNPIQTITFNINKVMMFCGMKNGAIRVYVLNQNDP
  SLTSLVDYWHFNMHDNNYGCIKSIANSFDDRFLVTAGADGNIFVFNIFSEFMLRKDMKAKVPSPRFGIET
  EPIPEDIEDPKAYSIENARRKREHDKLMKEVGEIKARKREQIKALRSEFCNLLEMNEKLPKHMQFKRTVS
  LI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018338
• ORF Size: 2946 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018338.2, NM_018338.3, NP_060808.2
• RefSeq Size: 3281 bp
• RefSeq ORF: 2949 bp
• Locus ID: 55779
• MW: 111.7 kDa