SERCA3 (ATP2A3) (NM_174955) Human Tagged ORF Clone

CAT#: RC220099

ATP2A3 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ATP2A3
• Synonyms: SERCA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220099 representing NM_174955
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGGCGCATCTGCTCCCGGCCGCCGACGTGCTGCGCCACTTCTCGGTGACAGCCGAGGGCGGCC
  TGAGCCCGGCGCAGGTGACCGGCGCGCGGGAGCGCTACGGCCCCAACGAGCTCCCGAGTGAGGAAGGGAA
  GTCCCTGTGGGAGCTGGTGCTGGAACAGTTTGAGGACCTCCTGGTGCGCATCCTGCTGCTGGCTGCCCTT
  GTCTCCTTTGTCCTGGCCTGGTTCGAGGAGGGCGAGGAGACCACGACCGCCTTCGTGGAGCCCCTGGTCA
  TCATGCTGATCCTCGTGGCCAACGCCATTGTGGGCGTGTGGCAGGAACGCAACGCCGAGAGTGCCATCGA
  GGCCCTGAAGGAGTATGAGCCTGAGATGGGCAAGGTGATCCGCTCGGACCGCAAGGGCGTGCAGAGGATC
  CGTGCCCGGGACATCGTCCCAGGGGACATTGTAGAAGTGGCAGTGGGGGACAAAGTGCCTGCTGACCTCC
  GCCTCATCGAGATCAAGTCCACCACGCTGCGAGTGGACCAGTCCATCCTGACGGGTGAATCTGTGTCCGT
  GACCAAGCACACAGAGGCCATCCCAGACCCCAGAGCTGTGAACCAGGACAAGAAGAACATGCTGTTTTCT
  GGCACCAATATCACATCGGGCAAAGCGGTGGGTGTGGCCGTGGCCACCGGCCTGCACACGGAGCTGGGCA
  AGATCCGGAGCCAGATGGCGGCAGTCGAGCCCGAGCGGACGCCGCTGCAGCGCAAGCTGGACGAGTTTGG
  ACGGCAGCTGTCCCACGCCATCTCTGTGATCTGCGTGGCCGTGTGGGTCATCAACATCGGCCACTTCGCC
  GACCCGGCCCACGGTGGCTCCTGGCTGCGTGGCGCTGTCTACTACTTCAAGATCGCCGTGGCCCTGGCGG
  TGGCGGCCATCCCCGAGGGCCTCCCGGCTGTCATCACTACATGCCTGGCACTGGGCACGCGGCGCATGGC
  ACGCAAGAACGCCATCGTGCGAAGCCTGCCGTCCGTGGAGACCCTGGGCTGCACCTCAGTCATCTGCTCC
  GACAAGACGGGCACGCTCACCACCAATCAGATGTCTGTCTGCCGGATGTTCGTGGTAGCCGAGGCCGATG
  CGGGCTCCTGCCTTTTGCACGAGTTCACCATCTCGGGTACCACGTATACCCCCGAGGGCGAAGTGCGGCA
  GGGGGATCAGCCTGTGCGCTGCGGCCAGTTCGACGGGCTGGTGGAGCTGGCGACCATCTGCGCCCTGTGC
  AACGACTCGGCTCTGGACTACAACGAGGCCAAGGGTGTGTATGAGAAGGTGGGAGAGGCCACGGAGACAG
  CTCTGACTTGCCTGGTGGAGAAGATGAACGTGTTCGACACCGACCTGCAGGCTCTGTCCCGGGTGGAGCG
  AGCTGGCGCCTGTAACACGGTCATCAAGCAGCTGATGCGGAAGGAGTTCACCCTGGAGTTCTCCCGAGAC
  CGGAAATCCATGTCCGTGTACTGCACGCCCACCCGCCCTCACCCTACTGGCCAGGGCAGCAAGATGTTTG
  TGAAGGGGGCTCCTGAGAGTGTGATCGAGCGCTGTAGCTCAGTCCGCGTGGGGAGCCGCACAGCACCCCT
  GACCCCCACCTCCAGGGAGCAGATCCTGGCAAAGATCCGGGATTGGGGCTCAGGCTCAGACACGCTGCGC
  TGCCTGGCACTGGCCACCCGGGACGCGCCCCCAAGGAAGGAGGACATGGAGCTGGACGACTGCAGCAAGT
  TTGTGCAGTACGAGACGGACCTGACCTTCGTGGGCTGCGTAGGCATGCTGGACCCGCCGCGACCTGAGGT
  GGCTGCCTGCATCACACGCTGCTACCAGGCGGGCATCCGCGTGGTCATGATCACGGGGGATAACAAAGGC
  ACTGCCGTGGCCATCTGCCGCAGGCTTGGCATCTTTGGGGACACGGAAGACGTGGCGGGCAAGGCCTACA
  CGGGCCGCGAGTTTGATGACCTCAGCCCCGAGCAGCAGCGCCAGGCCTGCCGCACCGCCCGCTGCTTCGC
  CCGCGTGGAGCCCGCACACAAGTCCCGCATCGTGGAGAACCTGCAGTCCTTTAACGAGATCACTGCTATG
  ACTGGCGATGGAGTGAACGACGCACCAGCCCTGAAGAAAGCAGAGATCGGCATCGCCATGGGCTCAGGCA
  CGGCCGTGGCCAAGTCGGCGGCAGAGATGGTGCTGTCAGATGACAACTTTGCCTCCATCGTGGCTGCGGT
  GGAGGAGGGCCGGGCCATCTACAGCAACATGAAGCAATTCATCCGCTACCTCATCTCCTCCAATGTTGGC
  GAGGTCGTCTGCATCTTCCTCACGGCAATTCTGGGCCTGCCCGAAGCCCTGATCCCTGTGCAGCTGCTCT
  GGGTGAACCTGGTGACAGACGGCCTACCTGCCACGGCTCTGGGCTTCAACCCGCCAGACCTGGACATCAT
  GGAGAAGCTGCCCCGGAGCCCCCGAGAAGCCCTCATCAGTGGCTGGCTCTTCTTCCGATACCTGGCTATC
  GGAGTGTACGTAGGCCTGGCCACAGTGGCTGCCGCCACCTGGTGGTTTGTGTATGACGCCGAGGGACCTC
  ACATCAACTTCTACCAGCTGAGGAACTTCCTGAAGTGCTCCGAAGACAACCCGCTCTTTGCCGGCATCGA
  CTGTGAGGTGTTCGAGTCACGCTTCCCCACCACCATGGCCTTGTCCGTGCTCGTGACCATTGAAATGTGC
  AATGCCCTCAACAGCGTCTCGGAGAACCAGTCGCTGCTGCGGATGCCGCCCTGGATGAACCCCTGGCTGC
  TGGTGGCTGTGGCCATGTCCATGGCCCTGCACTTCCTCATCCTGCTCGTGCCGCCCCTGCCTCTCATTTT
  CCAGGTGACCCCACTGAGCGGGCGCCAGTGGGTGGTGGTGCTCCAGATATCTCTGCCTGTCATCCTGCTG
  GATGAGGCCCTCAAGTACCTGTCCCGGAACCACATGCACGCCTGTCTTTATCCAGGCCTTCTCAGGACAG
  TCTCGCAGGCCTGGAGTAGGCAGCCGCTGACCACCTCTTGGACCCCAGACCACACCGGAAGAAATGAGCC
  AGAAGTGAGCGCTGGGAACAGAGTGGAGTCTCCGGTGTGTACCTCAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220099 representing NM_174955
  Red=Cloning site Green=Tags(s)
  
  MEAAHLLPAADVLRHFSVTAEGGLSPAQVTGARERYGPNELPSEEGKSLWELVLEQFEDLLVRILLLAAL
  VSFVLAWFEEGEETTTAFVEPLVIMLILVANAIVGVWQERNAESAIEALKEYEPEMGKVIRSDRKGVQRI
  RARDIVPGDIVEVAVGDKVPADLRLIEIKSTTLRVDQSILTGESVSVTKHTEAIPDPRAVNQDKKNMLFS
  GTNITSGKAVGVAVATGLHTELGKIRSQMAAVEPERTPLQRKLDEFGRQLSHAISVICVAVWVINIGHFA
  DPAHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICS
  DKTGTLTTNQMSVCRMFVVAEADAGSCLLHEFTISGTTYTPEGEVRQGDQPVRCGQFDGLVELATICALC
  NDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLQALSRVERAGACNTVIKQLMRKEFTLEFSRD
  RKSMSVYCTPTRPHPTGQGSKMFVKGAPESVIERCSSVRVGSRTAPLTPTSREQILAKIRDWGSGSDTLR
  CLALATRDAPPRKEDMELDDCSKFVQYETDLTFVGCVGMLDPPRPEVAACITRCYQAGIRVVMITGDNKG
  TAVAICRRLGIFGDTEDVAGKAYTGREFDDLSPEQQRQACRTARCFARVEPAHKSRIVENLQSFNEITAM
  TGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYSNMKQFIRYLISSNVG
  EVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEKLPRSPREALISGWLFFRYLAI
  GVYVGLATVAAATWWFVYDAEGPHINFYQLRNFLKCSEDNPLFAGIDCEVFESRFPTTMALSVLVTIEMC
  NALNSVSENQSLLRMPPWMNPWLLVAVAMSMALHFLILLVPPLPLIFQVTPLSGRQWVVVLQISLPVILL
  DEALKYLSRNHMHACLYPGLLRTVSQAWSRQPLTTSWTPDHTGRNEPEVSAGNRVESPVCTSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_174955
• ORF Size: 3129 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_174955.1, NM_174955.2, NP_777615.1
• RefSeq Size: 4827
• RefSeq ORF: 3132
• Locus ID: 489
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway
• MW: 114 kDa
• Gene Summary: This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]