TAS2R46 (NM_176887) Human Tagged ORF Clone

CAT#: RC220115

TAS2R46 (Myc-DDK-tagged)-Human taste receptor, type 2, member 46 (TAS2R46)

Product Images

Western blot validation of overexpression lysate (Cat# LY406141) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220115 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAS2R46
• Synonyms: T2R46; T2R54
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220115 representing NM_176887
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAACTTTTCTGCCCATCATTTTTTCCATTCTAATAGTGGTTACATTTGTGATTGGAAATTTTGCTA
  ATGGCTTCATAGCATTGGTAAATTCCATTGAGTGGTTCAAGAGACAAAAGATCTCTTTTGCTGACCAAAT
  TCTCACTGCTCTGGCAGTCTCCAGAGTTGGTTTACTCTGGGTATTAGTATTAAATTGGTATGCAACTGAG
  TTGAATCCAGCTTTTAACAGTATAGAAGTAAGAATTACTGCTTACAATGTCTGGGCAGTAATCAACCATT
  TCAGCAACTGGCTTGCTACTAGCCTCAGCATATTTTATTTGCTCAAGATTGCCAATTTCTCCAACCTTAT
  TTTTCTTCACTTAAAGAGGAGAGTTAAGAGTGTTGTTCTGGTGATACTATTGGGGCCTTTGCTATTTTTG
  GTTTGTCATCTTTTTGTGATAAACATGAATCAGATTATATGGACAAAAGAATATGAAGGAAACATGACTT
  GGAAGATCAAACTGAGGAGTGCAATGTACCTTTCAAATACAACGGTAACCATCCTAGCAAACTTAGTTCC
  CTTCACTCTGACCCTGATATCTTTTCTGCTGTTAATCTGTTCTCTGTGTAAACATCTCAAAAAGATGCAG
  CTCCATGGCAAAGGATCTCAAGATCCCAGCATGAAGGTCCACATAAAAGCTTTGCAAACTGTGACCTCCT
  TCCTCTTGTTATGTGCCATTTACTTTCTGTCCATAATCATGTCAGTTTGGAGTTTTGAGAGTCTGGAAAA
  CAAACCTGTCTTCATGTTCTGCGAAGCTATTGCATTCAGCTATCCTTCAACCCACCCATTCATCCTGATT
  TGGGGAAACAAGAAGCTAAAGCAGACTTTTCTTTCAGTTTTGTGGCATGTGAGGTACTGGGTGAAAGGAG
  AGAAGCCTTCATCTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220115 representing NM_176887
  Red=Cloning site Green=Tags(s)
  
  MITFLPIIFSILIVVTFVIGNFANGFIALVNSIEWFKRQKISFADQILTALAVSRVGLLWVLVLNWYATE
  LNPAFNSIEVRITAYNVWAVINHFSNWLATSLSIFYLLKIANFSNLIFLHLKRRVKSVVLVILLGPLLFL
  VCHLFVINMNQIIWTKEYEGNMTWKIKLRSAMYLSNTTVTILANLVPFTLTLISFLLLICSLCKHLKKMQ
  LHGKGSQDPSMKVHIKALQTVTSFLLLCAIYFLSIIMSVWSFESLENKPVFMFCEAIAFSYPSTHPFILI
  WGNKKLKQTFLSVLWHVRYWVKGEKPSSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176887
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_176887.2, NP_795368.2
• RefSeq Size: 930
• RefSeq ORF: 930
• Locus ID: 259292
• Protein Pathways: Taste transduction
• MW: 35.3 kDa
• Gene Summary: TAS2R46 belongs to the large TAS2R receptor family. TAS2Rs are expressed on the surface of taste receptor cells and mediate the perception of bitterness through a G protein-coupled second messenger pathway (Conte et al., 2002 [PubMed 12584440]). For further information on TAS2Rs, see MIM 604791. [supplied by OMIM, Sep 2009]