COCH (NM_004086) Human Tagged ORF Clone
CAT#: RC220182L1
- LentiORF®
Lenti-ORF clone of COCH (Myc-DDK-tagged)-Human coagulation factor C homolog, cochlin (Limulus polyphemus) (COCH), transcript variant 2
"NM_004086" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | COCH |
Synonyms | COCH-5B2; COCH5B2; DFNA9; DFNB110 |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC220182).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_004086 |
ORF Size | 1635 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004086.2 |
RefSeq Size | 2558 bp |
RefSeq ORF | 1653 bp |
Locus ID | 1690 |
UniProt ID | O43405 |
Cytogenetics | 14q12 |
Domains | VWA, LCCL |
MW | 58.8 kDa |
Gene Summary | The protein encoded by this gene is highly conserved in human, mouse, and chicken, showing 94% and 79% amino acid identity of human to mouse and chicken sequences, respectively. Hybridization to this gene was detected in spindle-shaped cells located along nerve fibers between the auditory ganglion and sensory epithelium. These cells accompany neurites at the habenula perforata, the opening through which neurites extend to innervate hair cells. This and the pattern of expression of this gene in chicken inner ear paralleled the histologic findings of acidophilic deposits, consistent with mucopolysaccharide ground substance, in temporal bones from DFNA9 (autosomal dominant nonsyndromic sensorineural deafness 9) patients. Mutations that cause DFNA9 have been reported in this gene. Alternative splicing results in multiple transcript variants encoding the same protein. Additional splice variants encoding distinct isoforms have been described but their biological validities have not been demonstrated. [provided by RefSeq, Oct 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC313960 | COCH (untagged)-Human coagulation factor C homolog, cochlin (Limulus polyphemus) (COCH), transcript variant 2 |
USD 930.00 |
|
RC220182 | COCH (Myc-DDK-tagged)-Human coagulation factor C homolog, cochlin (Limulus polyphemus) (COCH), transcript variant 2 |
USD 480.00 |
|
RG220182 | COCH (GFP-tagged) - Human coagulation factor C homolog, cochlin (Limulus polyphemus) (COCH), transcript variant 2 |
USD 530.00 |
|
RC220182L2 | Lenti-ORF clone of COCH (mGFP-tagged)-Human coagulation factor C homolog, cochlin (Limulus polyphemus) (COCH), transcript variant 2 |
USD 680.00 |
|
RC220182L3 | Lenti-ORF clone of COCH (Myc-DDK-tagged)-Human coagulation factor C homolog, cochlin (Limulus polyphemus) (COCH), transcript variant 2 |
USD 680.00 |
|
RC220182L4 | Lenti-ORF clone of COCH (mGFP-tagged)-Human coagulation factor C homolog, cochlin (Limulus polyphemus) (COCH), transcript variant 2 |
USD 680.00 |
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