BTF3 (NM_001037637) Human Tagged ORF Clone
CAT#: RC220184
BTF3 (Myc-DDK-tagged)-Human basic transcription factor 3 (BTF3), transcript variant 1
"NM_001037637" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | BTF3 |
Synonyms | BETA-NAC; BTF3a; BTF3b; NACB |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220184 representing NM_001037637
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGACGGACAGGCGCACCCGCTCAGGCTGACTCTCGGGGGCGAGGTCGAGCCAGGGGCGGCTGCCCTG GGGGCGAGGCGACGCTGTCTCAACCTCCACCTCGCGGCGGAACCCGAGGACAGGAGCCTCAGATGAAAGA AACAATCATGAACCAGGAAAAACTCGCCAAACTGCAGGCACAAGTGCGCATTGGTGGGAAAGGAACTGCT CGCAGAAAGAAGAAGGTGGTTCATAGAACAGCCACAGCAGATGACAAAAAACTTCAGTTCTCCTTAAAGA AGTTAGGGGTAAACAATATCTCTGGTATTGAAGAGGTGAATATGTTTACAAACCAAGGAACAGTGATCCA CTTTAACAACCCTAAAGTTCAGGCATCTCTGGCAGCGAACACTTTCACCATTACAGGCCATGCTGAGACA AAGCAGCTGACAGAAATGCTACCCAGCATCTTAAACCAGCTTGGTGCGGATAGTCTGACTAGTTTAAGGA GACTGGCCGAAGCTCTGCCCAAACAATCTGTGGATGGAAAAGCACCACTTGCTACTGGAGAGGATGATGA TGATGAAGTTCCAGATCTTGTGGAGAATTTTGATGAGGCTTCCAAGAATGAGGCAAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220184 representing NM_001037637
Red=Cloning site Green=Tags(s) MRRTGAPAQADSRGRGRARGGCPGGEATLSQPPPRGGTRGQEPQMKETIMNQEKLAKLQAQVRIGGKGTA RRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAET KQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNEAN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001037637 |
ORF Size | 618 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001037637.1, NP_001032726.1 |
RefSeq Size | 1262 bp |
RefSeq ORF | 621 bp |
Locus ID | 689 |
Cytogenetics | 5q13.2 |
Protein Families | Transcription Factors |
MW | 22 kDa |
Gene Summary | This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC302918 | BTF3 (untagged)-Human basic transcription factor 3 (BTF3), transcript variant 1 |
USD 420.00 |
|
RG220184 | BTF3 (GFP-tagged) - Human basic transcription factor 3 (BTF3), transcript variant 1 |
USD 460.00 |
|
RC220184L3 | Lenti ORF clone of Human basic transcription factor 3 (BTF3), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC220184L4 | Lenti ORF clone of Human basic transcription factor 3 (BTF3), transcript variant 1, mGFP tagged |
USD 620.00 |
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